Syntabulin Protein (AA 1-663) (Strep Tag)

Produktnummer ABIN3118591

Produktdetails

Target: Syntabulin (SYBU) (Syntabulin (Syntaxin-Interacting) (SYBU))

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-663

Spezies: Human

Quelle: Tobacco (Nicotiana tabacum)

Aufreinigungstag / Konjugat: Dieses Syntabulin Protein ist gelabelt mit Strep Tag.

Applikation: ELISA, SDS-PAGE (SDS), Western Blotting (WB)

Sequenz: MGPLRESKKE HRVQHHDKEI SRSRIPRLIL RPHMPQQQHK VSPASESPFS EEESREFNPS SSGRSARTVS SNSFCSDDTG CPSSQSVSPV KTPSDAGNSP IGFCPGSDEG FTRKKCTIGM VGEGSIQSSR YKKESKSGLV KPGSEADFSS SSSTGSISAP EVHMSTAGSK RSSSSRNRGP HGRSNGASSH KPGSSPSSPR EKDLLSMLCR NQLSPVNIHP SYAPSSPSSS NSGSYKGSDC SPIMRRSGRY MSCGENHGVR PPNPEQYLTP LQQKEVTVRH LKTKLKESER RLHERESEIV ELKSQLARMR EDWIEEECHR VEAQLALKEA RKEIKQLKQV IETMRSSLAD KDKGIQKYFV DINIQNKKLE SLLQSMEMAH SGSLRDELCL DFPCDSPEKS LTLNPPLDTM ADGLSLEEQV TGEGADRELL VGDSIANSTD LFDEIVTATT TESGDLELVH STPGANVLEL LPIVMGQEEG SVVVERAVQT DVVPYSPAIS ELIQSVLQKL QDPCPSSLAS PDESEPDSME SFPESLSALV VDLTPRNPNS AILLSPVETP YANVDAEVHA NRLMRELDFA ACVEERLDGV IPLARGGVVR QYWSSSFLVD LLAVAAPVVP TVLWAFSTQR GGTDPVYNIG ALLRGCCVVA LHSLRRTAFR IKT
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Produktmerkmale: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin-Niveau: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Kommentare:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: Syntabulin (SYBU) (Syntabulin (Syntaxin-Interacting) (SYBU))

Andere Bezeichnung: SYBU (SYBU Produkte)

Synonyme: GOLSYN Protein, OCSYN Protein, SNPHL Protein, Golsyn Protein, Ocsyn Protein, RGD1564335 Protein, 5730410E15Rik Protein, A830027B17Rik Protein, mKIAA1472 Protein, fi06c06 Protein, syn Protein, wu:fi06c06 Protein, zgc:153017 Protein, golsyn Protein, ocsyn Protein, snphl Protein, sybu Protein, syntabulin Protein, syntabulin (syntaxin-interacting) Protein, syntabulin S homeolog Protein, SYBU Protein, Sybu Protein, sybu Protein, sybu.S Protein

Hintergrund: Syntabulin (Golgi-localized syntaphilin-related protein) (Syntaxin-1-binding protein),FUNCTION: Part of a kinesin motor-adapter complex that is critical for the anterograde axonal transport of active zone components and contributes to activity-dependent presynaptic assembly during neuronal development. {ECO:0000250, ECO:0000269|PubMed:15459722}.

Molekulargewicht: 72.4 kDa

UniProt: Q9NX95