CD163L1 Protein (AA 41-1453) (rho-1D4 tag)

Produktnummer ABIN3118426

Produktdetails

Target: CD163L1 (CD163 Molecule-Like 1 (CD163L1))

Protein-Typ: Recombinant

Proteineigenschaft: AA 41-1453

Spezies: Human

Quelle: Insektenzellen

Aufreinigungstag / Konjugat: Dieses CD163L1 Protein ist gelabelt mit rho-1D4 tag.

Applikation: Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB)

Sequenz: FNGTDLELRL VNGDGPCSGT VEVKFQGQWG TVCDDGWNTT ASTVVCKQLG CPFSFAMFRF GQAVTRHGKI WLDDVSCYGN ESALWECQHR EWGSHNCYHG EDVGVNCYGE ANLGLRLVDG NNSCSGRVEV KFQERWGTIC DDGWNLNTAA VVCRQLGCPS SFISSGVVNS PAVLRPIWLD DILCQGNELA LWNCRHRGWG NHDCSHNEDV TLTCYDSSDL ELRLVGGTNR CMGRVELKIQ GRWGTVCHHK WNNAAADVVC KQLGCGTALH FAGLPHLQSG SDVVWLDGVS CSGNESFLWD CRHSGTVNFD CLHQNDVSVI CSDGADLELR LADGSNNCSG RVEVRIHEQW WTICDQNWKN EQALVVCKQL GCPFSVFGSR RAKPSNEARD IWINSISCTG NESALWDCTY DGKAKRTCFR RSDAGVICSD KADLDLRLVG AHSPCYGRLE VKYQGEWGTV CHDRWSTRNA AVVCKQLGCG KPLHVFGMTY FKEASGPIWL DDVSCIGNES NIWDCEHSGW GKHNCVHRED VIVTCSGDAT WGLRLVGGSN RCSGRLEVYF QGRWGTVCDD GWNSKAAAVV CSQLDCPSSI IGMGLGNAST GYGKIWLDDV SCDGDESDLW SCRNSGWGNN DCSHSEDVGV ICSDASDMEL RLVGGSSRCA GKVEVNVQGA VGILCANGWG MNIAEVVCRQ LECGSAIRVS REPHFTERTL HILMSNSGCT GGEASLWDCI RWEWKQTACH LNMEASLICS AHRQPRLVGA DMPCSGRVEV KHADTWRSVC DSDFSLHAAN VLCRELNCGD AISLSVGDHF GKGNGLTWAE KFQCEGSETH LALCPIVQHP EDTCIHSREV GVVCSRYTDV RLVNGKSQCD GQVEINVLGH WGSLCDTHWD PEDARVLCRQ LSCGTALSTT GGKYIGERSV RVWGHRFHCL GNESLLDNCQ MTVLGAPPCI HGNTVSVICT GSLTQPLFPC LANVSDPYLS AVPEGSALIC LEDKRLRLVD GDSRCAGRVE IYHDGFWGTI CDDGWDLSDA HVVCQKLGCG VAFNATVSAH FGEGSGPIWL DDLNCTGMES HLWQCPSRGW GQHDCRHKED AGVICSEFTA LRLYSETETE SCAGRLEVFY NGTWGSVGRR NITTAIAGIV CRQLGCGENG VVSLAPLSKT GSGFMWVDDI QCPKTHISIW QCLSAPWERR ISSPAEETWI TCEDRIRVRG GDTECSGRVE IWHAGSWGTV CDDSWDLAEA EVVCQQLGCG SALAALRDAS FGQGTGTIWL DDMRCKGNES FLWDCHAKPW GQSDCGHKED AGVRCSGQSL KSLNASSGHL ALILSSIFGL LLLVLFILFL TWCRVQKQKH LPLRVSTRRR GSLEENLFHE METCLKREDP HGTRTSDDTP NHGCEDASDT SLLGVLPASE ATK
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Produktmerkmale:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human CD163L1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Three step purification of membrane proteins expressed in baculovirus infected SF9 insect cells:

1. Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot.
2. The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot.
3. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Protein is endotoxin-free.

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Kommentare:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: CD163L1 (CD163 Molecule-Like 1 (CD163L1))

Andere Bezeichnung: CD163L1 (CD163L1 Produkte)

Synonyme: CD163B Protein, M160 Protein, B430307C05 Protein, E430002D04Rik Protein, Scart1 Protein, CD163 molecule like 1 Protein, CD163 molecule-like 1 Protein, CD163L1 Protein, Cd163l1 Protein

Molekulargewicht: 155.9 kDa Including tag.

UniProt: Q9NR16