ATP1A1 Protein (AA 6-1023) (rho-1D4 tag)

Produktnummer ABIN3111887

Produktdetails

Target: ATP1A1 (Sodium Potassium ATPase, alpha1 (ATP1A1))

Protein-Typ: Recombinant

Proteineigenschaft: AA 6-1023

Spezies: Human

Quelle: Insektenzellen

Aufreinigungstag / Konjugat: Dieses ATP1A1 Protein ist gelabelt mit rho-1D4 tag.

Applikation: SDS-PAGE (SDS), Western Blotting (WB), Crystallization (Crys), ELISA

Sequenz: GRDKYEPAAV SEQGDKKGKK GKKDRDMDEL KKEVSMDDHK LSLDELHRKY GTDLSRGLTS ARAAEILARD GPNALTPPPT TPEWIKFCRQ LFGGFSMLLW IGAILCFLAY SIQAATEEEP QNDNLYLGVV LSAVVIITGC FSYYQEAKSS KIMESFKNMV PQQALVIRNG EKMSINAEEV VVGDLVEVKG GDRIPADLRI ISANGCKVDN SSLTGESEPQ TRSPDFTNEN PLETRNIAFF STNCVEGTAR GIVVYTGDRT VMGRIATLAS GLEGGQTPIA AEIEHFIHII TGVAVFLGVS FFILSLILEY TWLEAVIFLI GIIVANVPEG LLATVTVCLT LTAKRMARKN CLVKNLEAVE TLGSTSTICS DKTGTLTQNR MTVAHMWFDN QIHEADTTEN QSGVSFDKTS ATWLALSRIA GLCNRAVFQA NQENLPILKR AVAGDASESA LLKCIELCCG SVKEMRERYA KIVEIPFNST NKYQLSIHKN PNTSEPQHLL VMKGAPERIL DRCSSILLHG KEQPLDEELK DAFQNAYLEL GGLGERVLGF CHLFLPDEQF PEGFQFDTDD VNFPIDNLCF VGLISMIDPP RAAVPDAVGK CRSAGIKVIM VTGDHPITAK AIAKGVGIIS EGNETVEDIA ARLNIPVSQV NPRDAKACVV HGSDLKDMTS EQLDDILKYH TEIVFARTSP QQKLIIVEGC QRQGAIVAVT GDGVNDSPAL KKADIGVAMG IAGSDVSKQA ADMILLDDNF ASIVTGVEEG RLIFDNLKKS IAYTLTSNIP EITPFLIFII ANIPLPLGTV TILCIDLGTD MVPAISLAYE QAESDIMKRQ PRNPKTDKLV NERLISMAYG QIGMIQALGG FFTYFVILAE NGFLPIHLLG LRVDWDDRWI NDVEDSYGQQ WTYEQRKIVE FTCHTAFFVS IVVVQWADLV ICKTRRNSVF QQGMKNKILI FGLFEETALA AFLSYCPGMG VALRMYPLKP TWWFCAFPYS LLIFVYDEVR KLIIRRRPGG WVEKETYY
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Produktmerkmale:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human ATP1A1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Three step purification of membrane proteins expressed in baculovirus infected SF9 insect cells:

1. Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot.
2. The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot.
3. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Protein is endotoxin-free.

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Kommentare:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: ATP1A1 (Sodium Potassium ATPase, alpha1 (ATP1A1))

Andere Bezeichnung: ATP1A1 (ATP1A1 Produkte)

Synonyme: Atpa-1 Protein, BC010319 Protein, Nkaa1b Protein, K-ATPase Protein, Na+K+ATPase Protein, atp1a1 Protein, atp1a1a.1 Protein, atp1a1l1 Protein, atp[a]1B1 Protein, cb70 Protein, wu:fa03c03 Protein, wu:fb92h10 Protein, atp1a1l5 Protein, atp[a]1B2 Protein, cb707 Protein, wu:fj34g01 Protein, zgc:92351 Protein, ATPase Na+/K+ transporting subunit alpha 1 Protein, ATPase, Na+/K+ transporting, alpha 1 polypeptide Protein, ATPase Na+/K+ transporting subunit alpha 1 L homeolog Protein, ATPase, Na+/K+ transporting, alpha 1a polypeptide, tandem duplicate 1 Protein, ATPase, Na+/K+ transporting, alpha 1b polypeptide Protein, ATP1A1 Protein, Atp1a1 Protein, atp1a1.L Protein, atp1a1a.1 Protein, atp1a1b Protein

Hintergrund: This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients.

Molekulargewicht: 113.6 kDa Including tag.

UniProt: P05023

Pathways: Thyroid Hormone Synthesis, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Proton Transport, Ribonucleoside Biosynthetic Process