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Transmembrane Protein 167A (TMEM167A) (AA 27-72) protein (rho-1D4 tag)

Crystallography grade TMEM167A Spezies: Human Wirt: Insektenzellen Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. Crys, ELISA, SDS, WB
Produktnummer ABIN3104194
  • Target Alle Transmembrane Protein 167A (TMEM167A) Produkte
    Transmembrane Protein 167A (TMEM167A)
    Protein-Typ
    Recombinant
    Proteineigenschaft
    AA 27-72
    Spezies
    Human
    Quelle
    • 1
    Insektenzellen
    Aufreinigungstag / Konjugat
    rho-1D4 tag
    Applikation
    Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB)
    Sequenz
    LAPSLLDRNK TGLLGIFWKC ARIGERKSPY VAVCCIVMAF SILFIQ
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Produktmerkmale
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Human TMEM167A Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Aufreinigung
    Three step purification of membrane proteins expressed in baculovirus infected SF9 insect cells:
    1. Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot.
    2. The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot.
    3. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Reinheit
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterilität
    0.22 μm filtered
    Endotoxin-Niveau
    Protein is endotoxin-free.
    Güteklasse
    Crystallography grade
  • Applikationshinweise
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
    Kommentare

    In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handhabung
    Avoid repeated freeze-thaw cycles.
    Lagerung
    -80 °C
    Informationen zur Lagerung
    Store at -80°C.
    Haltbarkeit
    Unlimited (if stored properly)
  • Target
    Transmembrane Protein 167A (TMEM167A)
    Andere Bezeichnung
    TMEM167A (TMEM167A Produkte)
    Synonyme
    0610041E09Rik Protein, 5730424F14Rik Protein, AU041184 Protein, AW537806 Protein, ENSMUSG00000060669 Protein, Gm10085 Protein, Tmem167a Protein, TMEM167 Protein, kish-a Protein, tmem167 Protein, fa97d03 Protein, wu:fa97d03 Protein, zgc:112458 Protein, transmembrane protein 167 Protein, transmembrane protein 167A Protein, transmembrane protein 167A L homeolog Protein, Tmem167 Protein, TMEM167A Protein, tmem167a.L Protein, tmem167a Protein
    Hintergrund
    Involved in the early part of the secretory pathway. {ECO:0000269|PubMed:19942856}.
    Molekulargewicht
    6.3 kDa Including tag.
    UniProt
    Q8TBQ9
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