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ATP2C1 Protein (AA 313-699) (His tag)

Crystallography grade ATP2C1 Spezies: Human Wirt: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Produktnummer ABIN3099226
  • Target Alle ATP2C1 Proteine anzeigen
    ATP2C1 (ATPase, Ca++ Transporting, Type 2C, Member 1 (ATP2C1))
    Protein-Typ
    Recombinant
    Proteineigenschaft
    AA 313-699
    Spezies
    • 5
    • 3
    Human
    Quelle
    • 5
    • 2
    • 1
    Escherichia coli (E. coli)
    Aufreinigungstag / Konjugat
    Dieses ATP2C1 Protein ist gelabelt mit His tag.
    Applikation
    Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)
    Sequenz
    MVVTVTLALG VMRMVKKRAI VKKLPIVETL GCCNVICSDK TGTLTKNEMT VTHIFTSDGL H AEVTGVGYNQ FGEVIVDGDV VHGFYNPAVS RIVEAGCVCN DAVIRNNTLM GKPTEGALIA LAMKMGLDGL QQDYIRKAEY PFSSEQKWMA VKCVHRTQQD RPEICFMKGA YEQVIKYCTT YQSKGQTLTL TQQQRDVYQQ EKARMGSAGL RVLALASGPE LGQLTFLGLV GIIDPPRTGV KEAVTTLIAS GVSIKMITGD SQETAVAIAS RLGLYSKTSQ SVSGEEIDAM DVQQLSQIVP KVAVFYRASP RHKMKIIKSL QKNGSVVAMT GDGVN
    Sequence includes C-terminal His-tag
    Produktmerkmale
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Human PLP1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Aufreinigung
    The protein is purified from the cleared cell lysate using His-tag capture materials. Eluate fractions are analyzed by SDS-PAGE. Protein containing fractions are subjected to a second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Reinheit
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterilität
    0.22 μm filtered
    Endotoxin-Niveau
    Protein is endotoxin free.
    Güteklasse
    Crystallography grade
  • Applikationshinweise
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
    Kommentare

    In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Buffer
    In solution (30 mM Hepes, pH 8.0, 100 mM NaCl, Laurylsarcosine)
    Handhabung
    Avoid repeated freeze-thaw cycles.
    Lagerung
    -80 °C
    Informationen zur Lagerung
    Store at -80°C.
    Haltbarkeit
    Unlimited (if stored properly)
  • Chernyavsky, Amber, Agnoletti, Wang, Grando: "Synergy among non-desmoglein antibodies contributes to the immunopathology of desmoglein antibody-negative pemphigus vulgaris." in: The Journal of biological chemistry, Vol. 294, Issue 12, pp. 4520-4528, (2019) (PubMed).

  • Target
    ATP2C1 (ATPase, Ca++ Transporting, Type 2C, Member 1 (ATP2C1))
    Andere Bezeichnung
    ATP2C1 (ATP2C1 Produkte)
    Synonyme
    ATP2C1 Protein, ATP2C1A Protein, BCPM Protein, HHD Protein, PMR1 Protein, SPCA1 Protein, hSPCA1 Protein, Spca1 Protein, si:dkey-11p23.6 Protein, SPCA Protein, 1700121J11Rik Protein, AW061228 Protein, D930003G21Rik Protein, pmr1 Protein, ATPase secretory pathway Ca2+ transporting 1 Protein, ATPase, Ca++ transporting, type 2C, member 1 Protein, ATPase, Ca++-sequestering Protein, ATP2C1 Protein, atp2c1 Protein, Atp2c1 Protein
    Hintergrund
    This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of the calcium.
    Molekulargewicht
    42.7 kDa including tag.
    UniProt
    P98194
    Pathways
    Transition Metal Ion Homeostasis, Ribonucleoside Biosynthetic Process
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