SLC30A1 Protein (AA 1-507) (Strep Tag)

Produktnummer ABIN3098169

Produktdetails

Target: SLC30A1 (Solute Carrier Family 30 (Zinc Transporter), Member 1 (SLC30A1))

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-507

Spezies: Human

Quelle: Tobacco (Nicotiana tabacum)

Aufreinigungstag / Konjugat: Dieses SLC30A1 Protein ist gelabelt mit Strep Tag.

Applikation: SDS-PAGE (SDS), Western Blotting (WB), ELISA

Sequenz: MGCWGRNRGR LLCMLALTFM FMVLEVVVSR VTSSLAMLSD SFHMLSDVLA LVVALVAERF ARRTHATQKN TFGWIRAEVM GALVNAIFLT GLCFAILLEA IERFIEPHEM QQPLVVLGVG VAGLLVNVLG LCLFHHHSGF SQDSGHGHSH GGHGHGHGLP KGPRVKSTRP GSSDINVAPG EQGPDQEETN TLVANTSNSN GLKLDPADPE NPRSGDTVEV QVNGNLVREP DHMELEEDRA GQLNMRGVFL HVLGDALGSV IVVVNALVFY FSWKGCSEGD FCVNPCFPDP CKAFVEIINS THASVYEAGP CWVLYLDPTL CVVMVCILLY TTYPLLKESA LILLQTVPKQ IDIRNLIKEL RNVEGVEEVH ELHVWQLAGS RIIATAHIKC EDPTSYMEVA KTIKDVFHNH GIHATTIQPE FASVGSKSSV VPCELACRTQ CALKQCCGTL PQAPSGKDAE KTPAVSISCL ELSNNLEKKP RRTKAENIPA VVIEIKNMPN KQPESSL
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Produktmerkmale: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin-Niveau: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Kommentare:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: SLC30A1 (Solute Carrier Family 30 (Zinc Transporter), Member 1 (SLC30A1))

Andere Bezeichnung: SLC30A1 (SLC30A1 Produkte)

Synonyme: ZNT1 Protein, ZRC1 Protein, AI839647 Protein, C130040I11Rik Protein, Znt1 Protein, ZnT1 Protein, slc30 Protein, MGC53047 Protein, XZnSLC30 Protein, slc30a1 Protein, zgc:77589 Protein, zrc1 Protein, ZnT-1 Protein, ZNT8 Protein, ZnT-8 Protein, zinc transporter 1 precursor Protein, RGD1305098 Protein, zgc:162909 Protein, znt1 Protein, solute carrier family 30 member 1 Protein, solute carrier family 30 (zinc transporter), member 1 Protein, solute carrier family 30 member 1 L homeolog Protein, solute carrier family 30 (zinc transporter), member 1a Protein, solute carrier family 30 member 8 Protein, zinc transporter 1 precursor Protein, solute carrier family 30, member 10 Protein, solute carrier family 30 (zinc transporter), member 1b Protein, SLC30A1 Protein, Slc30a1 Protein, slc30a1.L Protein, slc30a1a Protein, slc30a1 Protein, SLC30A8 Protein, ZIP1 Protein, Slc30a10 Protein, slc30a1b Protein

Hintergrund: Proton-coupled zinc antiporter SLC30A1 (Solute carrier family 30 member 1) (Zinc transporter 1),FUNCTION: Zinc ion:proton antiporter that could function at the plasma membrane mediating zinc efflux from cells against its electrochemical gradient protecting them from intracellular zinc accumulation and toxicity (PubMed:31471319). Alternatively, could prevent the transport to the plasma membrane of CACNB2, the L-type calcium channels regulatory subunit, through a yet to be defined mechanism. By modulating the expression of these channels at the plasma membrane, could prevent calcium and zinc influx into cells. By the same mechanism, could also prevent L-type calcium channels-mediated heavy metal influx into cells (By similarity). In some cells, could also function as a zinc ion:proton antiporter mediating zinc entry into the lumen of cytoplasmic vesicles. In macrophages, can increase zinc ions concentration into the lumen of cytoplasmic vesicles containing engulfed bacteria and could help inactivate them (PubMed:32441444). {ECO:0000250|UniProtKB:Q62720, ECO:0000269|PubMed:31471319, ECO:0000269|PubMed:32441444}.

Molekulargewicht: 55.3 kDa

UniProt: Q9Y6M5

Pathways: Transition Metal Ion Homeostasis