ZNF518A Protein (AA 1-1483) (Strep Tag)

Produktnummer ABIN3096495

Produktdetails

Target: ZNF518A (Zinc Finger Protein 518A (ZNF518A))

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-1483

Spezies: Human

Quelle: Tobacco (Nicotiana tabacum)

Aufreinigungstag / Konjugat: Dieses ZNF518A Protein ist gelabelt mit Strep Tag.

Applikation: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Sequenz: MPSEQKQLFC DEKQTTLKKD YDVKNEIVDR SAPKPKISGS IHYALKNVKI DLPKINIPNE VLLKHEVDKY RKLFQSKQQT ARKSISIKTV SCVEECTLLH KSERAEEEGV KMSAKILNFS CLKCRDNTRY SPNDLQKHFQ MWHHGELPSY PCEMCNFSAN DFQVFKQHRR THRSTLVKCD ICNNESVYTL LNLTKHFTST HCVNGNFQCE KCKFSTQDVG TFVQHIHRHN EIHYKCGKCH HVCFTKGELQ KHLHIHSGTF PFTCQYCSYG ATRREHLVRH VITLHKEHLY AKEKLEKDKY EKRMAKTSAG LKLILKRYKI GASRKTFWKR KKINSGSDRS IEKNTQVLKK MNKTQTKSED QSHVVQEHLS EEKDERLHCE NNDKAPESES EKPTPLSTGQ GNRAEEGPNA SSGFMKTAVL GPTLKNVMMK NNKLAVSPNY NATFMGFKMM DGKQHIVLKL VPIKQNVCSP GSQSGAAKDG TANLQPQTLD TNGFLTGVTT ELNDTVYMKA ATPFSCSSSI LSGKASSEKE MTLISQRNNM LQTMDYEKSV SSLSATSELV TASVNLTTKF ETRDNVDFWG NHLTQSHPEV LGTTIKSPDK VNCVAKPNAY NSGDMHNYCI NYGNCELPVE SSNQGSLPFH NYSKVNNSNK RRRFSGTAVY ENPQRESSSS KTVVQQPISE SFLSLVRQES SKPDSLLASI SLLNDKDGTL KAKSEIEEQY VLEKGQNIDG QNLYSNENQN LECATEKSKW EDFSNVDSPM MPRITSVFSL QSQQASEFLP PEVNQLLQDV LKIKPDVKQD SSNTPNKGLP LHCDQSFQKH EREGKIVESS KDFKVQGIFP VPPGSVGINV PTNDLNLKFG KEKQVSSIPQ DVRDSEKMPR ISGFGTLLKT QSDAIITQQL VKDKLRATTQ NLGSFYMQSP LLNSEQKKTI IVQTSKGFLI PLNITNKPGL PVIPGNALPL VNSQGIPASL FVNKKPGMVL TLNNGKLEGV SAVKTEGAPA RGTVTKEPCK TPILKVEPNN NCLTPGLCSS IGSCLSMKSS SENTLPLKGP YILKPTSSVK AVLIPNMLSE QQSTKLNISD SVKQQNEIFP KPPLYTFLPD GKQAVFLKCV MPNKTELLKP KLVQNSTYQN IQPKKPEGTP QRILLKIFNP VLNVTAANNL SVSNSASSLQ KDNVPSNQII GGEQKEPESR DALPFLLDDL MPANEIVITS TATCPESSEE PICVSDCSES RVLRCKTNCR IERNFNRKKT SKKIFSKTKT HGSKDSETAF VSRNRNCKRK CRDSYQEPPR RKATLHRKCK EKAKPEDVRE TFGFSRPRLS KDSIRTLRLF PFSSKQLVKC PRRNQPVVVL NHPDADAPEV VSVMKTIAKF NGHVLKVSLS KRTINALLKP VCYNPPKTTY DDFSKRHKTF KPVSSVKERF VLKLTLKKTS KNNYQIVKTT SENILKAKFN CWFCGRVFDN QDTWAGHGQR HLMEATRDWN MLE
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Produktmerkmale: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin-Niveau: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Kommentare:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: ZNF518A (Zinc Finger Protein 518A (ZNF518A))

Andere Bezeichnung: ZNF518A (ZNF518A Produkte)

Synonyme: ZNF518 Protein, 2810401C22Rik Protein, 6330417C12Rik Protein, AI426493 Protein, Zfp518 Protein, Znf518 Protein, Znf518a Protein, mKIAA0335 Protein, RGD1305314 Protein, zinc finger protein 518A Protein, ZNF518A Protein, Zfp518a Protein

Hintergrund: Zinc finger protein 518A,FUNCTION: Through its association with the EHMT1-EHMT2/G9A and PRC2/EED-EZH2 histone methyltransferase complexes may function in gene silencing, regulating repressive post-translational methylation of histone tails at promoters of target genes. {ECO:0000250|UniProtKB:B2RRF6}.

Molekulargewicht: 166.8 kDa

UniProt: Q6AHZ1