THBS4 Protein (AA 27-961) (His tag)

Produktnummer ABIN3096142

Produktdetails

Target: THBS4 (Thrombospondin 4 (THBS4))

Protein-Typ: Recombinant

Proteineigenschaft: AA 27-961

Spezies: Human

Quelle: Insektenzellen

Aufreinigungstag / Konjugat: Dieses THBS4 Protein ist gelabelt mit His tag.

Applikation: Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)

Sequenz: TPQVFDLLPS SSQRLNPGAL LPVLTDPALN DLYVISTFKL QTKSSATIFG LYSSTDNSKY FEFTVMGRLN KAILRYLKND GKVHLVVFNN LQLADGRRHR ILLRLSNLQR GAGSLELYLD CIQVDSVHNL PRAFAGPSQK PETIELRTFQ RKPQDFLEEL KLVVRGSLFQ VASLQDCFLQ QSEPLAATGT GDFNRQFLGQ MTQLNQLLGE VKDLLRQQVK ETSFLRNTIA ECQACGPLKF QSPTPSTVVP PAPPAPPTRP PRRCDSNPCF RGVQCTDSRD GFQCGPCPEG YTGNGITCID VDECKYHPCY PGVHCINLSP GFRCDACPVG FTGPMVQGVG ISFAKSNKQV CTDIDECRNG ACVPNSICVN TLGSYRCGPC KPGYTGDQIR GCKAERNCRN PELNPCSVNA QCIEERQGDV TCVCGVGWAG DGYICGKDVD IDSYPDEELP CSARNCKKDN CKYVPNSGQE DADRDGIGDA CDEDADGDGI LNEQDNCVLI HNVDQRNSDK DIFGDACDNC LSVLNNDQKD TDGDGRGDAC DDDMDGDGIK NILDNCPKFP NRDQRDKDGD GVGDACDSCP DVSNPNQSDV DNDLVGDSCD TNQDSDGDGH QDSTDNCPTV INSAQLDTDK DGIGDECDDD DDNDGIPDLV PPGPDNCRLV PNPAQEDSNS DGVGDICESD FDQDQVIDRI DVCPENAEVT LTDFRAYQTV VLDPEGDAQI DPNWVVLNQG MEIVQTMNSD PGLAVGYTAF NGVDFEGTFH VNTQTDDDYA GFIFGYQDSS SFYVVMWKQT EQTYWQATPF RAVAEPGIQL KAVKSKTGPG EHLRNSLWHT GDTSDQVRLL WKDSRNVGWK DKVSYRWFLQ HRPQVGYIRV RFYEGSELVA DSGVTIDTTM RGGRLGVFCF SQENIIWSNL KYRCNDTIPE DFQEFQTQNF DRFDN
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Produktmerkmale:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human THBS4 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Protein is endotoxin free.

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Kommentare:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: THBS4 (Thrombospondin 4 (THBS4))

Andere Bezeichnung: THBS4 (THBS4 Produkte)

Synonyme: TSP4 Protein, tsp4 Protein, thbs4 Protein, zgc:111910 Protein, MGC79977 Protein, TSP-4 Protein, zgc:175276 Protein, thrombospondin 4 Protein, thrombospondin 4b Protein, thrombospondin 4 S homeolog Protein, thrombospondin-4 Protein, thrombospondin 4a Protein, THBS4 Protein, Thbs4 Protein, thbs4b Protein, thbs4.S Protein, thbs4 Protein, thbs4a Protein

Hintergrund: Adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions and is involved in various processes including cellular proliferation, migration, adhesion and attachment, inflammatory response to CNS injury, regulation of vascular inflammation and adaptive responses of the heart to pressure overload and in myocardial function and remodeling. Binds to structural extracellular matrix (ECM) proteins and modulates the ECM in response to tissue damage, contributing to cardioprotective and adaptive ECM remodeling. Plays a role in ER stress response, via its interaction with the activating transcription factor 6 alpha (ATF6) which produces adaptive ER stress response factors and protects myocardium from pressure overload. May contribute to spinal presynaptic hypersensitivity and neuropathic pain states after peripheral nerve injury. May play a role in regulating protective astrogenesis from the subventricular zone (SVZ) niche after injury in a NOTCH1-dependent manner (By similarity). {ECO:0000250, ECO:0000269|PubMed:19441079}.

Molekulargewicht: 104.1 kDa Including tag.

UniProt: P35443