THO Complex 3 Protein (THOC3) (AA 2-351) (His tag)

Produktnummer ABIN3095899

Produktdetails

Target: THO Complex 3 (THOC3)

Protein-Typ: Recombinant

Proteineigenschaft: AA 2-351

Spezies: Human

Quelle: Insektenzellen

Aufreinigungstag / Konjugat: Dieses THO Complex 3 Protein ist gelabelt mit His tag.

Applikation: ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)

Sequenz: AVPAAAMGPS ALGQSGPGSM APWCSVSSGP SRYVLGMQEL FRGHSKTREF LAHSAKVHSV AWSCDGRRLA SGSFDKTASV FLLEKDRLVK ENNYRGHGDS VDQLCWHPSN PDLFVTASGD KTIRIWDVRT TKCIATVNTK GENINICWSP DGQTIAVGNK DDVVTFIDAK THRSKAEEQF KFEVNEISWN NDNNMFFLTN GNGCINILSY PELKPVQSIN AHPSNCICIK FDPMGKYFAT GSADALVSLW DVDELVCVRC FSRLDWPVRT LSFSHDGKML ASASEDHFID IAEVETGDKL WEVQCESPTF TVAWHPKRPL LAFACDDKDG KYDSSREAGT VKLFGLPNDS
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Produktmerkmale:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human THOC3 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Protein is endotoxin free.

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Kommentare:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: THO Complex 3 (THOC3)

Andere Bezeichnung: THOC3 (THOC3 Produkte)

Synonyme: THOC3 Protein, zgc:100815 Protein, THO3 Protein, hTREX45 Protein, 2410044K02Rik Protein, AL033344 Protein, THO complex 3 Protein, THO complex subunit 3 Protein, THOC3 Protein, thoc3 Protein, LOC100400596 Protein, Thoc3 Protein

Hintergrund: Required for efficient export of polyadenylated RNA and spliced mRNA. Acts as component of the THO subcomplex of the TREX complex which is thought to couple mRNA transcription, processing and nuclear export, and which specifically associates with spliced mRNA and not with unspliced pre-mRNA. TREX is recruited to spliced mRNAs by a transcription-independent mechanism, binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export to the cytoplasm via the TAP/NFX1 pathway. The TREX complex is essential for the export of Kaposi's sarcoma-associated herpesvirus (KSHV) intronless mRNAs and infectious virus production. {ECO:0000269|PubMed:11979277, ECO:0000269|PubMed:15833825, ECO:0000269|PubMed:15998806, ECO:0000269|PubMed:17190602, ECO:0000269|PubMed:18974867}.

Molekulargewicht: 39.6 kDa Including tag.

UniProt: Q96J01