VARS Protein (AA 2-1264) (His tag)

Produktnummer ABIN3095796

Produktdetails

Target: VARS (Valyl-tRNA Synthetase (VARS))

Protein-Typ: Recombinant

Proteineigenschaft: AA 2-1264

Spezies: Human

Quelle: Insektenzellen

Aufreinigungstag / Konjugat: Dieses VARS Protein ist gelabelt mit His tag.

Applikation: Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)

Sequenz: STLYVSPHPD AFPSLRALIA ARYGEAGEGP GWGGAHPRIC LQPPPTSRTP FPPPRLPALE QGPGGLWVWG ATAVAQLLWP AGLGGPGGSR AAVLVQQWVS YADTELIPAA CGATLPALGL RSSAQDPQAV LGALGRALSP LEEWLRLHTY LAGEAPTLAD LAAVTALLLP FRYVLDPPAR RIWNNVTRWF VTCVRQPEFR AVLGEVVLYS GARPLSHQPG PEAPALPKTA AQLKKEAKKR EKLEKFQQKQ KIQQQQPPPG EKKPKPEKRE KRDPGVITYD LPTPPGEKKD VSGPMPDSYS PRYVEAAWYP WWEQQGFFKP EYGRPNVSAA NPRGVFMMCI PPPNVTGSLH LGHALTNAIQ DSLTRWHRMR GETTLWNPGC DHAGIATQVV VEKKLWREQG LSRHQLGREA FLQEVWKWKE EKGDRIYHQL KKLGSSLDWD RACFTMDPKL SAAVTEAFVR LHEEGIIYRS TRLVNWSCTL NSAISDIEVD KKELTGRTLL SVPGYKEKVE FGVLVSFAYK VQGSDSDEEV VVATTRIETM LGDVAVAVHP KDTRYQHLKG KNVIHPFLSR SLPIVFDEFV DMDFGTGAVK ITPAHDQNDY EVGQRHGLEA ISIMDSRGAL INVPPPFLGL PRFEARKAVL VALKERGLFR GIEDNPMVVP LCNRSKDVVE PLLRPQWYVR CGEMAQAASA AVTRGDLRIL PEAHQRTWHA WMDNIREWCI SRQLWWGHRI PAYFVTVSDP AVPPGEDPDG RYWVSGRNEA EAREKAAKEF GVSPDKISLQ QDEDVLDTWF SSGLFPLSIL GWPNQSEDLS VFYPGTLLET GHDILFFWVA RMVMLGLKLT GRLPFREVYL HAIVRDAHGR KMSKSLGNVI DPLDVIYGIS LQGLHNQLLN SNLDPSEVEK AKEGQKADFP AGIPECGTDA LRFGLCAYMS QGRDINLDVN RILGYRHFCN KLWNATKFAL RGLGKGFVPS PTSQPGGHES LVDRWIRSRL TEAVRLSNQG FQAYDFPAVT TAQYSFWLYE LCDVYLECLK PVLNGVDQVA AECARQTLYT CLDVGLRLLS PFMPFVTEEL FQRLPRRMPQ APPSLCVTPY PEPSECSWKD PEAEAALELA LSITRAVRSL RADYNLTRIR PDCFLEVADE ATGALASAVS GYVQALASAG VVAVLALGAP APQGCAVALA SDRCSIHLQL QGLVDPAREL GKLQAKRVEA QRQAQRLRER RAASGYPVKV PLEVQEADEA KLQQTEAELR KVDEAIALFQ KML
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Produktmerkmale:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human VARS Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Protein is endotoxin free.

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Kommentare:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: VARS (Valyl-tRNA Synthetase (VARS))

Andere Bezeichnung: VARS (VARS Produkte)

Synonyme: G7A Protein, VARS1 Protein, VARS2 Protein, VARS Protein, Bat6 Protein, D17H6S56E Protein, G7a Protein, Vars2 Protein, vars1 Protein, vars2 Protein, CHUNP6879 Protein, im:7137378 Protein, wu:fb07a11 Protein, wu:fb07d10 Protein, TrsVal Protein, BEST:LD45671 Protein, CG4062 Protein, Dmel\\CG4062 Protein, VRS Protein, ValRS Protein, l(2)03531 Protein, l(2)rI255 Protein, ld45671 Protein, ECK4251 Protein, JW4215 Protein, val-act Protein, T5E21.11 Protein, T5E21_11 Protein, TWIN 2 Protein, VALRS Protein, VALYL TRNA SYNTHETASE Protein, valyl-tRNA synthetase Protein, Valyl-tRNA synthetase Protein, valyl-tRNA synthetase S homeolog Protein, hypothetical protein Protein, valyl-tRNA synthetase 2, mitochondrial Protein, valyl-tRNA synthetase / valine-tRNA ligase (VALRS) Protein, VARS Protein, Vars Protein, vars Protein, ValRS Protein, vars.S Protein, MW0078 Protein, Pnap_3169 Protein, valS Protein, Fnod_0343 Protein, Cyan7425_1070 Protein, Tola_0553 Protein, Slip_0680 Protein, Astex_3136 Protein, ML5_3395 Protein, VARS2 Protein, TWN2 Protein

Molekulargewicht: 141.3 kDa Including tag.

UniProt: P26640