SYNM Protein (AA 1-1565) (Strep Tag)

Produktnummer ABIN3095786

Produktdetails

Target: SYNM (Synemin, Intermediate Filament Protein (SYNM))

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-1565

Spezies: Human

Quelle: Tobacco (Nicotiana tabacum)

Aufreinigungstag / Konjugat: Dieses SYNM Protein ist gelabelt mit Strep Tag.

Applikation: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Sequenz: MLSWRLQTGP EKAELQELNA RLYDYVCRVR ELERENLLLE EELRGRRGRE GLWAEGQARC AEEARSLRQQ LDELSWATAL AEGERDALRR ELRELQRLDA EERAARGRLD AELGAQQREL QEALGARAAL EALLGRLQAE RRGLDAAHER DVRELRARAA SLTMHFRARA TGPAAPPPRL REVHDSYALL VAESWRETVQ LYEDEVRELE EALRRGQESR LQAEEETRLC AQEAEALRRE ALGLEQLRAR LEDALLRMRE EYGIQAEERQ RVIDCLEDEK ATLTLAMADW LRDYQDLLQV KTGLSLEVAT YRALLEGESN PEIVIWAEHV ENMPSEFRNK SYHYTDSLLQ RENERNLFSR QKAPLASFNH SSALYSNLSG HRGSQTGTSI GGDARRGFLG SGYSSSATTQ QENSYGKAVS SQTNVRTFSP TYGLLRNTEA QVKTFPDRPK AGDTREVPVY IGEDSTIARE SYRDRRDKVA AGASESTRSN ERTVILGKKT EVKATREQER NRPETIRTKP EEKMFDSKEK ASEERNLRWE ELTKLDKEAR QRESQQMKEK AKEKDSPKEK SVREREVPIS LEVSQDRRAE VSPKGLQTPV KDAGGGTGRE AEARELRFRL GTSDATGSLQ GDSMTETVAE NIVTSILKQF TQSPETEASA DSFPDTKVTY VDRKELPGER KTKTEIVVES KLTEDVDVSD EAGLDYLLSK DIKEVGLKGK SAEQMIGDII NLGLKGREGR AKVVNVEIVE EPVSYVSGEK PEEFSVPFKV EEVEDVSPGP WGLVKEEEGY GESDVTFSVN QHRRTKQPQE NTTHVEEVTE AGDSEGEQSY FVSTPDEHPG GHDRDDGSVY GQIHIEEEST IRYSWQDEIV QGTRRRTQKD GAVGEKVVKP LDVPAPSLEG DLGSTHWKEQ ARSGEFHAEP TVIEKEIKIP HEFHTSMKGI SSKEPRQQLV EVIGQLEETL PERMREELSA LTREGQGGPG SVSVDVKKVQ GAGGSSVTLV AEVNVSQTVD ADRLDLEELS KDEASEMEKA VESVVRESLS RQRSPAPGSP DEEGGAEAPA AGIRFRRWAT RELYIPSGES EVAGGASHSS GQRTPQGPVS ATVEVSSPTG FAQSQVLEDV SQAARHIKLG PSEVWRTERM SYEGPTAEVV EVSAGGDLSQ AASPTGASRS VRHVTLGPGQ SPLSREVIFL GPAPACPEAW GSPEPGPAES SADMDGSGRH STFGCRQFHA EKEIIFQGPI SAAGKVGDYF ATEESVGTQT SVRQLQLGPK EGFSGQIQFT APLSDKVELG VIGDSVHMEG LPGSSTSIRH ISIGPQRHQT TQQIVYHGLV PQLGESGDSE STVHGEGSAD VHQATHSHTS GRQTVMTEKS TFQSVVSESP QEDSAEDTSG AEMTSGVSRS FRHIRLGPTE TETSEHIAIR GPVSRTFVLA GSADSPELGK LADSSRTLRH IAPGPKETSF TFQMDVSNVE AIRSRTQEAG ALGVSDRGSW RDADSRNDQA VGVSFKASAG EGDQAHREQG KEQAMFDKKV QLQRMVDQRS VISDEKKVAL LYLDNEEEEN DGHWF
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Produktmerkmale: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin-Niveau: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Kommentare:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: SYNM (Synemin, Intermediate Filament Protein (SYNM))

Andere Bezeichnung: SYNM (SYNM Produkte)

Synonyme: DMN Protein, dmn Protein, si:dkey-25f3.3 Protein, SYN Protein, 4930412K21Rik Protein, AI852401 Protein, Dmn Protein, E130104F11 Protein, Syn Protein, Synemin Protein, synemin Protein, synemin, intermediate filament protein Protein, SYNM Protein, synm Protein, Synm Protein

Hintergrund: Synemin (Desmuslin),FUNCTION: Type-VI intermediate filament (IF) which plays an important cytoskeletal role within the muscle cell cytoskeleton. It forms heteromeric IFs with desmin and/or vimentin, and via its interaction with cytoskeletal proteins alpha-dystrobrevin, dystrophin, talin-1, utrophin and vinculin, is able to link these heteromeric IFs to adherens-type junctions, such as to the costameres, neuromuscular junctions, and myotendinous junctions within striated muscle cells. {ECO:0000269|PubMed:11353857, ECO:0000269|PubMed:16777071, ECO:0000269|PubMed:18028034}.

Molekulargewicht: 172.9 kDa

UniProt: O15061