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AARS2 Protein (AA 24-985) (His tag)

Crystallography grade AARS2 Spezies: Human Wirt: Insektenzellen Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, Crys, ELISA
Produktnummer ABIN3095729
  • Target Alle AARS2 Proteine anzeigen
    AARS2 (Alanyl-tRNA Synthetase 2, Mitochondrial (AARS2))
    Protein-Typ
    Recombinant
    Proteineigenschaft
    AA 24-985
    Spezies
    • 1
    • 1
    Human
    Quelle
    • 1
    • 1
    Insektenzellen
    Aufreinigungstag / Konjugat
    Dieses AARS2 Protein ist gelabelt mit His tag.
    Applikation
    SDS-PAGE (SDS), Western Blotting (WB), Crystallization (Crys), ELISA
    Sequenz
    GLSHRPLSSE PPAAKASAVR AAFLNFFRDR HGHRLVPSAS VRPRGDPSLL FVNAGMNQFK PIFLGTVDPR SEMAGFRRVA NSQKCVRAGG HHNDLEDVGR DLSHHTFFEM LGNWAFGGEY FKEEACNMAW ELLTQVYGIP EERLWISYFD GDPKAGLDPD LETRDIWLSL GVPASRVLSF GPQENFWEMG DTGPCGPCTE IHYDLAGGVG APQLVELWNL VFMQHNREAD GSLQPLPQRH VDTGMGLERL VAVLQGKHST YDTDLFSPLL NAIQQGCRAP PYLGRVGVAD EGRTDTAYRV VADHIRTLSV CISDGIFPGM SGPPLVLRRI LRRAVRFSME ILKAPPGFLG SLVPVVVETL GDAYPELQRN SAQIANLVSE DEAAFLASLE RGRRIIDRTL RTLGPSDMFP AEVAWSLSLC GDLGLPLDMV ELMLEEKGVQ LDSAGLERLA QEEAQHRARQ AEPVQKQGLW LDVHALGELQ RQGVPPTDDS PKYNYSLRPS GSYEFGTCEA QVLQLYTEDG TAVASVGKGQ RCGLLLDRTN FYAEQGGQAS DRGYLVRAGQ EDVLFPVARA QVCGGFILHE AVAPECLRLG DQVQLHVDEA WRLGCMAKHT ATHLLNWALR QTLGPGTEQQ GSHLNPEQLR LDVTTQTPLT PEQLRAVENT VQEAVGQDEA VYMEEVPLAL TAQVPGLRSL DEVYPDPVRV VSVGVPVAHA LDPASQAALQ TSVELCCGTH LLRTGAVGDL VIIGDRQLSK GTTRLLAVTG EQAQQARELG QSLAQEVKAA TERLSLGSRD VAEALRLSKD IGRLIEAVET AVMPQWQRRE LLATVKMLQR RANTAIRKLQ MGQAAKKTQE LLERHSKGPL IVDTVSAESL SVLVKVVRQL CEQAPSTSVL LLSPQPMGKV LCACQVAQGA MPTFTAEAWA LAVCSHMGGK AWGSRVVAQG TGSTTDLEAA LSIAQTYALS QL
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Produktmerkmale
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Human AARS2 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Aufreinigung
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Reinheit
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterilität
    0.22 μm filtered
    Endotoxin-Niveau
    Protein is endotoxin free.
    Güteklasse
    Crystallography grade
  • Applikationshinweise
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Kommentare

    In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handhabung
    Avoid repeated freeze-thaw cycles.
    Lagerung
    -80 °C
    Informationen zur Lagerung
    Store at -80°C.
    Haltbarkeit
    Unlimited (if stored properly)
  • Target
    AARS2 (Alanyl-tRNA Synthetase 2, Mitochondrial (AARS2))
    Andere Bezeichnung
    AARS2 (AARS2 Produkte)
    Synonyme
    AARSL Protein, COXPD8 Protein, MT-ALARS Protein, MTALARS Protein, Aarsl Protein, AlaRS Protein, Gm89 Protein, alanyl-tRNA synthetase 2, mitochondrial Protein, AARS2 Protein, Aars2 Protein
    Hintergrund
    Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged tRNA(Ala) via its editing domain. {ECO:0000255|HAMAP-Rule:MF_03133}.
    Molekulargewicht
    105.7 kDa Including tag.
    UniProt
    Q5JTZ9
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