STIL Protein (AA 1-1287) (Strep Tag)

Produktnummer ABIN3095690

Produktdetails

Target: STIL (SCL/TAL1 Interrupting Locus (STIL))

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-1287

Spezies: Human

Quelle: Tobacco (Nicotiana tabacum)

Aufreinigungstag / Konjugat: Dieses STIL Protein ist gelabelt mit Strep Tag.

Applikation: ELISA, SDS-PAGE (SDS), Western Blotting (WB)

Sequenz: MEPIYPFARP QMNTRFPSSR MVPFHFPPSK CALWNPTPTG DFIYLHLSYY RNPKLVVTEK TIRLAYRHAK QNKKNSSCFL LGSLTADEDE EGVTLTVDRF DPGREVPECL EITPTASLPG DFLIPCKVHT QELCSREMIV HSVDDFSSAL KALQCHICSK DSLDCGKLLS LRVHITSRES LDSVEFDLHW AAVTLANNFK CTPVKPIPII PTALARNLSS NLNISQVQGT YKYGYLTMDE TRKLLLLLES DPKVYSLPLV GIWLSGITHI YSPQVWACCL RYIFNSSVQE RVFSESGNFI IVLYSMTHKE PEFYECFPCD GKIPDFRFQL LTSKETLHLF KNVEPPDKNP IRCELSAESQ NAETEFFSKA SKNFSIKRSS QKLSSGKMPI HDHDSGVEDE DFSPRPIPSP HPVSQKISKI QPSVPELSLV LDGNFIESNP LPTPLEMVNN ENPPLINHLE HLKPLQPQLY DEKHSPEVEA GEPSLRGIPN QLNQDKPALL RHCKVRQPPA YKKGNPHTRN SIKPSSHNGP SHDIFEKLQT VSAGNVQNEE YPIRPSTLNS RQSSLAPQSQ PHDFVFSPHN SGRPMELQIP TPPLPSYCST NVCRCCQHHS HIQYSPLNSW QGANTVGSIQ DVQSEALQKH SLFHPSGCPA LYCNAFCSSS SPIALRPQGD MGSCSPHSNI EPSPVARPPS HMDLCNPQPC TVCMHTPKTE SDNGMMGLSP DAYRFLTEQD RQLRLLQAQI QRLLEAQSLM PCSPKTTAVE DTVQAGRQME LVSVEAQSSP GLHMRKGVSI AVSTGASLFW NAAGEDQEPD SQMKQDDTKI SSEDMNFSVD INNEVTSLPG SASSLKAVDI PSFEESNIAV EEEFNQPLSV SNSSLVVRKE PDVPVFFPSG QLAESVSMCL QTGPTGGASN NSETSEEPKI EHVMQPLLHQ PSDNQKIYQD LLGQVNHLLN SSSKETEQPS TKAVIISHEC TRTQNVYHTK KKTHHSRLVD KDCVLNATLK QLRSLGVKID SPTKVKKNAH NVDHASVLAC ISPEAVISGL NCMSFANVGM SGLSPNGVDL SMEANAIALK YLNENQLSQL SVTRSNQNNC DPFSLLHINT DRSTVGLSLI SPNNMSFATK KYMKRYGLLQ SSDNSEDEEE PPDNADSKSE YLLNQNLRSI PEQLGGQKEP SKNDHEIINC SNCESVGTNA DTPVLRNITN EVLQTKAKQQ LTEKPAFLVK NLKPSPAVNL RTGKAEFTQH PEKENEGDIT IFPESLQPSE TLKQMNSMNS VGTFLDVKRL RQLPKLF
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Produktmerkmale: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin-Niveau: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Kommentare:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: STIL (SCL/TAL1 Interrupting Locus (STIL))

Andere Bezeichnung: STIL (STIL Produkte)

Synonyme: Sil Protein, MCPH7 Protein, SIL Protein, chunp6907 Protein, sill Protein, zgc:110502 Protein, STIL, centriolar assembly protein Protein, Scl/Tal1 interrupting locus Protein, STIL, centriolar assembly protein L homeolog Protein, scl/tal1 interrupting locus Protein, Stil Protein, STIL Protein, stil.L Protein, stil Protein

Hintergrund: SCL-interrupting locus protein (TAL-1-interrupting locus protein),FUNCTION: Immediate-early gene. Plays an important role in embryonic development as well as in cellular growth and proliferation, its long-term silencing affects cell survival and cell cycle distribution as well as decreases CDK1 activity correlated with reduced phosphorylation of CDK1. Plays a role as a positive regulator of the sonic hedgehog pathway, acting downstream of PTCH1 (PubMed:16024801, PubMed:9372240). Plays an important role in the regulation of centriole duplication. Required for the onset of procentriole formation and proper mitotic progression. During procentriole formation, is essential for the correct loading of SASS6 and CENPJ to the base of the procentriole to initiate procentriole assembly (PubMed:22020124). In complex with STIL acts as a modulator of PLK4-driven cytoskeletal rearrangements and directional cell motility (PubMed:32107292, PubMed:29712910). {ECO:0000269|PubMed:16024801, ECO:0000269|PubMed:22020124, ECO:0000269|PubMed:29712910, ECO:0000269|PubMed:32107292, ECO:0000269|PubMed:9372240}.

Molekulargewicht: 143.0 kDa

UniProt: Q15468

Pathways: Tube Formation