SRRT Protein (AA 2-876) (His tag)

Produktnummer ABIN3095672

Produktdetails

Target: SRRT (Serrate RNA Effector Molecule Homolog (SRRT))

Protein-Typ: Recombinant

Proteineigenschaft: AA 2-876

Spezies: Human

Quelle: Insektenzellen

Aufreinigungstag / Konjugat: Dieses SRRT Protein ist gelabelt mit His tag.

Applikation: ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)

Sequenz: GDSDDEYDRR RRDKFRRERS DYDRSRERDE RRRGDDWNDR EWDRGRERRS RGEYRDYDRN RRERFSPPRH ELSPPQKRMR RDWDEHSSDP YHSGYEMPYA GGGGGPTYGP PQPWGHPDVH IMQHHVLPIQ ARLGSIAEID LGVPPPVMKT FKEFLLSLDD SVDETEAVKR YNDYKLDFRR QQMQDFFLAH KDEEWFRSKY HPDEVGKRRQ EARGALQNRL RVFLSLMETG WFDNLLLDID KADAIVKMLD AAVIKMEGGT ENDLRILEQE EEEEQAGKPG EPSKKEEGRA GAGLGDGERK TNDKDEKKED GKQAENDSSN DDKTKKSEGD GDKEEKKEDS EKEAKKSSKK RNRKHSGDDS FDEGSVSESE SESESGQAEE EKEEAEEALK EKEKPKEEEW EKPKDAAGLE CKPRPLHKTC SLFMRNIAPN ISRAEIISLC KRYPGFMRVA LSEPQPERRF FRRGWVTFDR SVNIKEICWN LQNIRLRECE LSPGVNRDLT RRVRNINGIT QHKQIVRNDI KLAAKLIHTL DDRTQLWASE PGTPPLPTSL PSQNPILKNI TDYLIEEVSA EEEELLGSSG GAPPEEPPKE GNPAEINVER DEKLIKVLDK LLLYLRIVHS LDYYNTCEYP NEDEMPNRCG IIHVRGPMPP NRISHGEVLE WQKTFEEKLT PLLSVRESLS EEEAQKMGRK DPEQEVEKFV TSNTQELGKD KWLCPLSGKK FKGPEFVRKH IFNKHAEKIE EVKKEVAFFN NFLTDAKRPA LPEIKPAQPP GPAQILPPGL TPGLPYPHQT PQGLMPYGQP RPPILGYGAG AVRPAVPTGG PPYPHAPYGA GRGNYDAFRG QGGYPGKPRN RMVRGDPRAI VEYRDLDAPD DVDFF
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Produktmerkmale:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human SRRT Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Protein is endotoxin free.

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Kommentare:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: SRRT (Serrate RNA Effector Molecule Homolog (SRRT))

Andere Bezeichnung: SRRT (SRRT Produkte)

Synonyme: CG7843 Protein, Dmel\\CG7843 Protein, anon-WO0118547.166 Protein, ars2 Protein, ars2-A Protein, serrate Protein, ARS2 Protein, ASR2 Protein, 2810019G02Rik Protein, ASR2A Protein, ASR2B Protein, ASR2C Protein, ASR2D Protein, Ars2 Protein, Asr2 Protein, MGC92060 Protein, CHUNP6881 Protein, zgc:92060 Protein, Arsenic resistance protein 2 Protein, serrate, RNA effector molecule S homeolog Protein, serrate, RNA effector molecule Protein, serrate RNA effector molecule homolog (Arabidopsis) Protein, Ars2 Protein, srrt.S Protein, SRRT Protein, Srrt Protein, srrt Protein

Hintergrund: Acts as a mediator between the cap-binding complex (CBC) and the primary microRNAs (miRNAs) processing machinery during cell proliferation. Contributes to the stability and delivery of capped primary miRNA transcripts to the primary miRNA processing complex containing DGCR8 and DROSHA, thereby playing a role in RNA-mediated gene silencing (RNAi) by miRNAs. Binds capped RNAs (m7GpppG-capped RNA), however interaction is probably mediated via its interaction with NCBP1/CBP80 component of the CBC complex. Involved in cell cycle progression at S phase. Does not directly confer arsenite resistance but rather modulates arsenic sensitivity. Independently of its activity on miRNAs, necessary and sufficient to promote neural stem cell self-renewal. Does so by directly binding SOX2 promoter and positively regulating its transcription (By similarity). {ECO:0000250, ECO:0000269|PubMed:19632182}.

Molekulargewicht: 101.5 kDa Including tag.

UniProt: Q9BXP5

Pathways: Notch Signalweg, Stem Cell Maintenance