SMO Protein (AA 28-787) (rho-1D4 tag)

Produktnummer ABIN3095608

Produktdetails

Target: SMO (Smoothened Homolog (Drosophila) (SMO))

Protein-Typ: Recombinant

Proteineigenschaft: AA 28-787

Spezies: Human

Quelle: Insektenzellen

Aufreinigungstag / Konjugat: Dieses SMO Protein ist gelabelt mit rho-1D4 tag.

Applikation: Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)

Sequenz: RGAASSGNAT GPGPRSAGGS ARRSAAVTGP PPPLSHCGRA APCEPLRYNV CLGSVLPYGA TSTLLAGDSD SQEEAHGKLV LWSGLRNAPR CWAVIQPLLC AVYMPKCEND RVELPSRTLC QATRGPCAIV ERERGWPDFL RCTPDRFPEG CTNEVQNIKF NSSGQCEVPL VRTDNPKSWY EDVEGCGIQC QNPLFTEAEH QDMHSYIAAF GAVTGLCTLF TLATFVADWR NSNRYPAVIL FYVNACFFVG SIGWLAQFMD GARREIVCRA DGTMRLGEPT SNETLSCVII FVIVYYALMA GVVWFVVLTY AWHTSFKALG TTYQPLSGKT SYFHLLTWSL PFVLTVAILA VAQVDGDSVS GICFVGYKNY RYRAGFVLAP IGLVLIVGGY FLIRGVMTLF SIKSNHPGLL SEKAASKINE TMLRLGIFGF LAFGFVLITF SCHFYDFFNQ AEWERSFRDY VLCQANVTIG LPTKQPIPDC EIKNRPSLLV EKINLFAMFG TGIAMSTWVW TKATLLIWRR TWCRLTGQSD DEPKRIKKSK MIAKAFSKRH ELLQNPGQEL SFSMHTVSHD GPVAGLAFDL NEPSADVSSA WAQHVTKMVA RRGAILPQDI SVTPVATPVP PEEQANLWLV EAEISPELQK RLGRKKKRRK RKKEVCPLAP PPELHPPAPA PSTIPRLPQL PRQKCLVAAG AWGAGDSCRQ GAWTLVSNPF CPEPSPPQDP FLPSAPAPVA WAHGRRQGLG PIHSRTNLMD TELMDADSDF
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Produktmerkmale:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human SMO Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Three step purification of membrane proteins expressed in baculovirus infected SF9 insect cells:

1. Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot.
2. The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot.
3. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Protein is endotoxin-free.

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Kommentare:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: SMO (Smoothened Homolog (Drosophila) (SMO))

Andere Bezeichnung: SMO (SMO Produkte)

Synonyme: FZD11 Protein, Gx Protein, SMOH Protein, Smoh Protein, E130215L21Rik Protein, bnb Protein, smoothened Protein, XSmo Protein, smoothened, frizzled class receptor Protein, smoothened, frizzled class receptor L homeolog Protein, SMO Protein, Smo Protein, smo.L Protein, smo Protein

Hintergrund: G protein-coupled receptor that probably associates with the patched protein (PTCH) to transduce the hedgehog's proteins signal. Binding of sonic hedgehog (SHH) to its receptor patched is thought to prevent normal inhibition by patched of smoothened (SMO). Required for the accumulation of KIF7 and GLI3 in the cilia. {ECO:0000269|PubMed:19592253}.

Molekulargewicht: 84.8 kDa Including tag.

UniProt: Q99835

Pathways: Hedgehog Signalweg