SENP6 Protein (AA 1-1112) (Strep Tag)

Produktnummer ABIN3095426

Produktdetails

Target: SENP6 (SUMO1/sentrin Specific Protease 6 (SENP6))

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-1112

Spezies: Human

Quelle: Tobacco (Nicotiana tabacum)

Aufreinigungstag / Konjugat: Dieses SENP6 Protein ist gelabelt mit Strep Tag.

Applikation: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Sequenz: MAAGKSGGSA GEITFLEALA RSESKRDGGF KNNWSFDHEE ESEGDTDKDG TNLLSVDEDE DSETSKGKKL NRRSEIVANS SGEFILKTYV RRNKSESFKT LKGNPIGLNM LSNNKKLSEN TQNTSLCSGT VVHGRRFHHA HAQIPVVKTA AQSSLDRKER KEYPPHVQKV EINPVRLSRL QGVERIMKKT EESESQVEPE IKRKVQQKRH CSTYQPTPPL SPASKKCLTH LEDLQRNCRQ AITLNESTGP LLRTSIHQNS GGQKSQNTGL TTKKFYGNNV EKVPIDIIVN CDDSKHTYLQ TNGKVILPGA KIPKITNLKE RKTSLSDLND PIILSSDDDD DNDRTNRRES ISPQPADSAC SSPAPSTGKV EAALNENTCR AERELRSIPE DSELNTVTLP RKARMKDQFG NSIINTPLKR RKVFSQEPPD ALALSCQSSF DSVILNCRSI RVGTLFRLLI EPVIFCLDFI KIQLDEPDHD PVEIILNTSD LTKCEWCNVR KLPVVFLQAI PAVYQKLSIQ LQMNKEDKVW NDCKGVNKLT NLEEQYIILI FQNGLDPPAN MVFESIINEI GIKNNISNFF AKIPFEEANG RLVACTRTYE ESIKGSCGQK ENKIKTVSFE SKIQLRSKQE FQFFDEEEET GENHTIFIGP VEKLIVYPPP PAKGGISVTN EDLHCLNEGE FLNDVIIDFY LKYLVLEKLK KEDADRIHIF SSFFYKRLNQ RERRNHETTN LSIQQKRHGR VKTWTRHVDI FEKDFIFVPL NEAAHWFLAV VCFPGLEKPK YEPNPHYHEN AVIQKCSTVE DSCISSSASE MESCSQNSSA KPVIKKMLNK KHCIAVIDSN PGQEESDPRY KRNICSVKYS VKKINHTASE NEEFNKGEST SQKVADRTKS ENGLQNESLS STHHTDGLSK IRLNYSDESP EAGKMLEDEL VDFSEDQDNQ DDSSDDGFLA DDNCSSEIGQ WHLKPTICKQ PCILLMDSLR GPSRSNVVKI LREYLEVEWE VKKGSKRSFS KDVMKGSNPK VPQQNNFSDC GVYVLQYVES FFENPILSFE LPMNLANWFP PPRMRTKREE IRNIILKLQE DQSKEKRKHK DTYSTEAPLG EGTEQYVNSI SD
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Produktmerkmale: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin-Niveau: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Kommentare:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: SENP6 (SUMO1/sentrin Specific Protease 6 (SENP6))

Andere Bezeichnung: SENP6 (SENP6 Produkte)

Synonyme: SSP1 Protein, SUSP1 Protein, 2810017C20Rik Protein, E130319N12Rik Protein, Susp1 Protein, mKIAA0797 Protein, fc83h02 Protein, im:7148342 Protein, wu:fc83h02 Protein, zgc:171230 Protein, SUMO1/sentrin specific peptidase 6 Protein, SUMO/sentrin specific peptidase 6 Protein, SUMO1/sentrin specific peptidase 6a Protein, SENP6 Protein, Senp6 Protein, senp6a Protein

Hintergrund: Sentrin-specific protease 6 (EC 3.4.22.-) (SUMO-1-specific protease 1) (Sentrin/SUMO-specific protease SENP6),FUNCTION: Protease that deconjugates SUMO1, SUMO2 and SUMO3 from targeted proteins. Processes preferentially poly-SUMO2 and poly-SUMO3 chains, but does not efficiently process SUMO1, SUMO2 and SUMO3 precursors. Deconjugates SUMO1 from RXRA, leading to transcriptional activation. Involved in chromosome alignment and spindle assembly, by regulating the kinetochore CENPH-CENPI-CENPK complex. Desumoylates PML and CENPI, protecting them from degradation by the ubiquitin ligase RNF4, which targets polysumoylated proteins for proteasomal degradation. Desumoylates also RPA1, thus preventing recruitment of RAD51 to the DNA damage foci to initiate DNA repair through homologous recombination. {ECO:0000269|PubMed:16912044, ECO:0000269|PubMed:17000875, ECO:0000269|PubMed:18799455, ECO:0000269|PubMed:20212317, ECO:0000269|PubMed:20705237, ECO:0000269|PubMed:21148299}.

Molekulargewicht: 126.1 kDa

UniProt: Q9GZR1