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SHANK2 Protein (AA 1-1470) (His tag)

Crystallography grade SHANK2 Spezies: Human Wirt: Insektenzellen Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, Crys, SDS
Produktnummer ABIN3095383
  • Target Alle SHANK2 Proteine anzeigen
    SHANK2 (SH3 and Multiple Ankyrin Repeat Domains 2 (SHANK2))
    Protein-Typ
    Recombinant
    Proteineigenschaft
    AA 1-1470
    Spezies
    • 1
    • 1
    Human
    Quelle
    • 1
    • 1
    Insektenzellen
    Aufreinigungstag / Konjugat
    Dieses SHANK2 Protein ist gelabelt mit His tag.
    Applikation
    ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)
    Sequenz
    MKSLLNAFTK KEVPFREAPA YSNRRRRPPN TLAAPRVLLR SNSDNNLNAS APDWAVCSTA TSHRSLSPQL LQQMPSKPEG AAKTIGSYVP GPRSRSPSLN RLGGAGEDGK RPQPLWHVGS PFALGANKDS LSAFEYPGPK RKLYSAVPGR LFVAVKPYQP QVDGEIPLHR GDRVKVLSIG EGGFWEGSAR GHIGWFPAEC VEEVQCKPRD SQAETRADRS KKLFRHYTVG SYDSFDTSSD CIIEEKTVVL QKKDNEGFGF VLRGAKADTP IEEFTPTPAF PALQYLESVD EGGVAWQAGL RTGDFLIEVN NENVVKVGHR QVVNMIRQGG NHLVLKVVTV TRNLDPDDTA RKKAPPPPKR APTTALTLRS KSMTSELEEL VDKASVRKKK DKPEEIVPAS KPSRAAENMA VEPRVATIKQ RPSSRCFPAG SDMNSVYERQ GIAVMTPTVP GSPKAPFLGI PRGTMRRQKS IDSRIFLSGI TEEERQFLAP PMLKFTRSLS MPDTSEDIPP PPQSVPPSPP PPSPTTYNCP KSPTPRVYGT IKPAFNQNSA AKVSPATRSD TVATMMREKG MYFRRELDRY SLDSEDLYSR NAGPQANFRN KRGQMPENPY SEVGKIASKA VYVPAKPARR KGMLVKQSNV EDSPEKTCSI PIPTIIVKEP STSSSGKSSQ GSSMEIDPQA PEPPSQLRPD ESLTVSSPFA AAIAGAVRDR EKRLEARRNS PAFLSTDLGD EDVGLGPPAP RTRPSMFPEE GDFADEDSAE QLSSPMPSAT PREPENHFVG GAEASAPGEA GRPLNSTSKA QGPESSPAVP SASSGTAGPG NYVHPLTGRL LDPSSPLALA LSARDRAMKE SQQGPKGEAP KADLNKPLYI DTKMRPSLDA GFPTVTRQNT RGPLRRQETE NKYETDLGRD RKGDDKKNML IDIMDTSQQK SAGLLMVHTV DATKLDNALQ EEDEKAEVEM KPDSSPSEVP EGVSETEGAL QISAAPEPTT VPGRTIVAVG SMEEAVILPF RIPPPPLASV DLDEDFIFTE PLPPPLEFAN SFDIPDDRAA SVPALSDLVK QKKSDTPQSP SLNSSQPTNS ADSKKPASLS NCLPASFLPP PESFDAVADS GIEEVDSRSS SDHHLETTST ISTVSSISTL SSEGGENVDT CTVYADGQAF MVDKPPVPPK PKMKPIIHKS NALYQDALVE EDVDSFVIPP PAPPPPPGSA QPGMAKVLQP RTSKLWGDVT EIKSPILSGP KANVISELNS ILQQMNREKL AKPGEGLDSP MGAKSASLAP RSPEIMSTIS GTRSTTVTFT VRPGTSQPIT LQSRPPDYES RTSGTRRAPS PVVSPTEMNK ETLPAPLSAA TASPSPALSD VFSLPSQPPS GDLFGLNPAG RSRSPSPSIL QQPISNKPFT TKPVHLWTKP DVADWLESLN LGEHKEAFMD NEIDGSHLPN LQKEDLIDLG VTRVGHRMNI ERALKQLLDR
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Produktmerkmale
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Human SHANK2 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Aufreinigung
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Reinheit
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterilität
    0.22 μm filtered
    Endotoxin-Niveau
    Protein is endotoxin free.
    Güteklasse
    Crystallography grade
    Top Product
    Discover our top product SHANK2 Protein
  • Applikationshinweise
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Kommentare

    In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handhabung
    Avoid repeated freeze-thaw cycles.
    Lagerung
    -80 °C
    Informationen zur Lagerung
    Store at -80°C.
    Haltbarkeit
    Unlimited (if stored properly)
  • Target
    SHANK2 (SH3 and Multiple Ankyrin Repeat Domains 2 (SHANK2))
    Andere Bezeichnung
    SHANK2 (SHANK2 Produkte)
    Synonyme
    AUTS17 Protein, CORTBP1 Protein, CTTNBP1 Protein, ProSAP1 Protein, SHANK Protein, SPANK-3 Protein, mKIAA1022 Protein, auts17 Protein, cortbp1 Protein, cttnbp1 Protein, prosap1 Protein, spank-3 Protein, shank2 Protein, MGC122482 Protein, CortBP1 Protein, Spank-3 Protein, SHANK2 Protein, SH3 and multiple ankyrin repeat domains 2 Protein, SH3 and multiple ankyrin repeat domains 2 L homeolog Protein, SH3 and multiple ankyrin repeat domains protein 2 Protein, SHANK2 Protein, Shank2 Protein, shank2.L Protein, shank2 Protein, LOC100438523 Protein
    Hintergrund
    Seems to be an adapter protein in the postsynaptic density (PSD) of excitatory synapses that interconnects receptors of the postsynaptic membrane including NMDA-type and metabotropic glutamate receptors, and the actin-based cytoskeleton. May play a role in the structural and functional organization of the dendritic spine and synaptic junction.
    Molekulargewicht
    159.8 kDa Including tag.
    UniProt
    Q9UPX8
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