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SREBF chaperone Protein (SCAP) (AA 1-1279) (rho-1D4 tag)

Crystallography grade SCAP Spezies: Human Wirt: Insektenzellen Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. Crys, ELISA, SDS, WB
Produktnummer ABIN3095307
  • Target Alle SREBF chaperone (SCAP) Proteine anzeigen
    SREBF chaperone (SCAP)
    Protein-Typ
    Recombinant
    Proteineigenschaft
    AA 1-1279
    Spezies
    • 4
    • 4
    Human
    Quelle
    • 8
    Insektenzellen
    Aufreinigungstag / Konjugat
    Dieses SREBF chaperone Protein ist gelabelt mit rho-1D4 tag.
    Applikation
    Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB)
    Sequenz
    MTLTERLREK ISRAFYNHGL LCASYPIPII LFTGFCILAC CYPLLKLPLP GTGPVEFTTP VKDYSPPPVD SDRKQGEPTE QPEWYVGAPV AYVQQIFVKS SVFPWHKNLL AVDVFRSPLS RAFQLVEEIR NHVLRDSSGI RSLEELCLQV TDLLPGLRKL RNLLPEHGCL LLSPGNFWQN DWERFHADPD IIGTIHQHEP KTLQTSATLK DLLFGVPGKY SGVSLYTRKR MVSYTITLVF QHYHAKFLGS LRARLMLLHP SPNCSLRAES LVHVHFKEEI GVAELIPLVT TYIILFAYIY FSTRKIDMVK SKWGLALAAV VTVLSSLLMS VGLCTLFGLT PTLNGGEIFP YLVVVIGLEN VLVLTKSVVS TPVDLEVKLR IAQGLSSESW SIMKNMATEL GIILIGYFTL VPAIQEFCLF AVVGLVSDFF LQMLFFTTVL SIDIRRMELA DLNKRLPPEA CLPSAKPVGQ PTRYERQLAV RPSTPHTITL QPSSFRNLRL PKRLRVVYFL ARTRLAQRLI MAGTVVWIGI LVYTDPAGLR NYLAAQVTEQ SPLGEGALAP MPVPSGMLPP SHPDPAFSIF PPDAPKLPEN QTSPGESPER GGPAEVVHDS PVPEVTWGPE DEELWRKLSF RHWPTLFSYY NITLAKRYIS LLPVIPVTLR LNPREALEGR HPQDGRSAWP PPGPIPAGHW EAGPKGPGGV QAHGDVTLYK VAALGLATGI VLVLLLLCLY RVLCPRNYGQ LGGGPGRRRR GELPCDDYGY APPETEIVPL VLRGHLMDIE CLASDGMLLV SCCLAGHVCV WDAQTGDCLT RIPRPGRQRR DSGVGSGLEA QESWERLSDG GKAGPEEPGD SPPLRHRPRG PPPPSLFGDQ PDLTCLIDTN FSAQPRSSQP TQPEPRHRAV CGRSRDSPGY DFSCLVQRVY QEEGLAAVCT PALRPPSPGP VLSQAPEDEG GSPEKGSPSL AWAPSAEGSI WSLELQGNLI VVGRSSGRLE VWDAIEGVLC CSSEEVSSGI TALVFLDKRI VAARLNGSLD FFSLETHTAL SPLQFRGTPG RGSSPASPVY SSSDTVACHL THTVPCAHQK PITALKAAAG RLVTGSQDHT LRVFRLEDSC CLFTLQGHSG AITTVYIDQT MVLASGGQDG AICLWDVLTG SRVSHVFAHR GDVTSLTCTT SCVISSGLDD LISIWDRSTG IKFYSIQQDL GCGASLGVIS DNLLVTGGQG CVSFWDLNYG DLLQTVYLGK NSEAQPARQI LVLDNAAIVC NFGSELSLVY VPSVLEKLD
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Produktmerkmale
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Human SCAP Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Aufreinigung
    Three step purification of membrane proteins expressed in baculovirus infected SF9 insect cells:
    1. Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot.
    2. The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot.
    3. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Reinheit
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterilität
    0.22 μm filtered
    Endotoxin-Niveau
    Protein is endotoxin-free.
    Güteklasse
    Crystallography grade
    Top Product
    Discover our top product SCAP Protein
  • Applikationshinweise
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Kommentare

    In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handhabung
    Avoid repeated freeze-thaw cycles.
    Lagerung
    -80 °C
    Informationen zur Lagerung
    Store at -80°C.
    Haltbarkeit
    Unlimited (if stored properly)
  • Target
    SREBF chaperone (SCAP)
    Andere Bezeichnung
    SCAP (SCAP Produkte)
    Synonyme
    9530044G19 Protein, mKIAA0199 Protein, SREBF chaperone Protein, SREBF chaperone S homeolog Protein, SCAP Protein, Scap Protein, scap.S Protein
    Hintergrund
    Escort protein required for cholesterol as well as lipid homeostasis. Regulates export of the SCAP/SREBF complex from the ER upon low cholesterol. Formation of a ternary complex with INSIG at high sterol concentrations leads to masking of an ER-export signal in SCAP and retention of the complex in the ER. Low sterol concentrations trigger release of INSIG, a conformational change in the SSC domain of SCAP, unmasking of the ER export signal, recruitment into COPII-coated vesicles, transport to the Golgi complex, proteolytic cleavage of SREBF in the Golgi, release of the transcription factor fragment of SREBF from the membrane, its import into the nucleus and up-regulation of LDLR, INSIG1 and the mevalonate pathway (By similarity). {ECO:0000250}.
    Molekulargewicht
    140.9 kDa Including tag.
    UniProt
    Q12770
    Pathways
    SARS-CoV-2 Protein Interaktom
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