SBNO1 Protein (AA 1-1393) (Strep Tag)

Produktnummer ABIN3095197

Produktdetails

Target: SBNO1 (Strawberry Notch Homolog 1 (SBNO1))

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-1393

Spezies: Human

Quelle: Tobacco (Nicotiana tabacum)

Aufreinigungstag / Konjugat: Dieses SBNO1 Protein ist gelabelt mit Strep Tag.

Applikation: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Sequenz: MVEPGQDLLL AALSESGISP NDLFDIDGGD AGLATPMPTP SVQQSVPLSA LELGLETEAA VPVKQEPETV PTPALLNVRQ QPPSTTTFVL NQINHLPPLG STIVMTKTPP VTTNRQTITL TKFIQTTAST RPSVSAPTVR NAMTSAPSKD QVQLKDLLKN NSLNELMKLK PPANIAQPVA TAATDVSNGT VKKESSNKEG ARMWINDMKM RSFSPTMKVP VVKEDDEPEE EDEEEMGHAE TYAEYMPIKL KIGLRHPDAV VETSSLSSVT PPDVWYKTSI SEETIDNGWL SALQLEAITY AAQQHETFLP NGDRAGFLIG DGAGVGKGRT IAGIIYENYL LSRKRALWFS VSNDLKYDAE RDLRDIGAKN ILVHSLNKFK YGKISSKHNG SVKKGVIFAT YSSLIGESQS GGKYKTRLKQ LLHWCGDDFD GVIVFDECHK AKNLCPVGSS KPTKTGLAVL ELQNKLPKAR VVYASATGAS EPRNMAYMNR LGIWGEGTPF REFSDFIQAV ERRGVGAMEI VAMDMKLRGM YIARQLSFTG VTFKIEEVLL SQSYVKMYNK AVKLWVIARE RFQQAADLID AEQRMKKSMW GQFWSAHQRF FKYLCIASKV KRVVQLAREE IKNGKCVVIG LQSTGEARTL EALEEGGGEL NDFVSTAKGV LQSLIEKHFP APDRKKLYSL LGIDLTAPSN NSSPRDSPCK ENKIKKRKGE EITREAKKAR KVGGLTGSSS DDSGSESDAS DNEESDYESS KNMSSGDDDD FNPFLDESNE DDENDPWLIR KDHKKNKEKK KKKSIDPDSI QSALLASGLG SKRPSFSSTP VISPAPNSTP ANSNTNSNSS LITSQDAVER AQQMKKDLLD KLEKLAEDLP PNTLDELIDE LGGPENVAEM TGRKGRVVSN DDGSISYESR SELDVPVEIL NITEKQRFMD GDKNIAIISE AASSGISLQA DRRAKNQRRR VHMTLELPWS ADRAIQQFGR THRSNQVTAP EYVFLISELA GEQRFASIVA KRLESLGALT HGDRRATESR DLSRFNFDNK YGRNALEIVM KSIVNLDSPM VSPPPDYPGE FFKDVRQGLI GVGLINVEDR SGILTLDKDY NNIGKFLNRI LGMEVHQQNA LFQYFADTLT AVVQNAKKNG RYDMGILDLG SGDEKVRKSD VKKFLTPGYS TSGHVELYTI SVERGMSWEE ATKIWAELTG PDDGFYLSLQ IRNNKKTAIL VKEVNPKKKL FLVYRPNTGK QLKLEIYADL KKKYKKVVSD DALMHWLDQY NSSADTCTHA YWRGNCKKAS LGLVCEIGLR CRTYYVLCGS VLSVWTKVEG VLASVSGTNV KMQIVRLRTE DGQRIVGLII PANCVSPLVN LLSTSDQSQQ LAVQQKQLWQ QHHPQSITNL SNA
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Produktmerkmale: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin-Niveau: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Kommentare:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: SBNO1 (Strawberry Notch Homolog 1 (SBNO1))

Andere Bezeichnung: SBNO1 (SBNO1 Produkte)

Synonyme: 9330180L10Rik Protein, AI849128 Protein, AW557836 Protein, BC021875 Protein, Sbno Protein, mSno1 Protein, sno Protein, MOP3 Protein, Sno Protein, strawberry notch homolog 1 (Drosophila) Protein, strawberry notch homolog 1 Protein, microRNA 8072 Protein, Sbno1 Protein, SBNO1 Protein, MIR8072 Protein

Hintergrund: Protein strawberry notch homolog 1 (Monocyte protein 3) (MOP-3)

Molekulargewicht: 154.3 kDa

UniProt: A3KN83

Pathways: SARS-CoV-2 Protein Interaktom