ROBO2 Protein (AA 22-1378) (rho-1D4 tag)

Produktnummer ABIN3095116

Produktdetails

Target: ROBO2 (Roundabout, Axon Guidance Receptor, Homolog 2 (ROBO2))

Protein-Typ: Recombinant

Proteineigenschaft: AA 22-1378

Spezies: Human

Quelle: Insektenzellen

Aufreinigungstag / Konjugat: Dieses ROBO2 Protein ist gelabelt mit rho-1D4 tag.

Applikation: ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)

Sequenz: SRLRQEDFPP RIVEHPSDVI VSKGEPTTLN CKAEGRPTPT IEWYKDGERV ETDKDDPRSH RMLLPSGSLF FLRIVHGRRS KPDEGSYVCV ARNYLGEAVS RNASLEVALL RDDFRQNPTD VVVAAGEPAI LECQPPRGHP EPTIYWKKDK VRIDDKEERI SIRGGKLMIS NTRKSDAGMY TCVGTNMVGE RDSDPAELTV FERPTFLRRP INQVVLEEEA VEFRCQVQGD PQPTVRWKKD DADLPRGRYD IKDDYTLRIK KTMSTDEGTY MCIAENRVGK MEASATLTVR APPQFVVRPR DQIVAQGRTV TFPCETKGNP QPAVFWQKEG SQNLLFPNQP QQPNSRCSVS PTGDLTITNI QRSDAGYYIC QALTVAGSIL AKAQLEVTDV LTDRPPPIIL QGPANQTLAV DGTALLKCKA TGDPLPVISW LKEGFTFPGR DPRATIQEQG TLQIKNLRIS DTGTYTCVAT SSSGETSWSA VLDVTESGAT ISKNYDLSDL PGPPSKPQVT DVTKNSVTLS WQPGTPGTLP ASAYIIEAFS QSVSNSWQTV ANHVKTTLYT VRGLRPNTIY LFMVRAINPQ GLSDPSPMSD PVRTQDISPP AQGVDHRQVQ KELGDVLVRL HNPVVLTPTT VQVTWTVDRQ PQFIQGYRVM YRQTSGLQAT SSWQNLDAKV PTERSAVLVN LKKGVTYEIK VRPYFNEFQG MDSESKTVRT TEEAPSAPPQ SVTVLTVGSY NSTSISVSWD PPPPDHQNGI IQEYKIWCLG NETRFHINKT VDAAIRSVII GGLFPGIQYR VEVAASTSAG VGVKSEPQPI IIGRRNEVVI TENNNSITEQ ITDVVKQPAF IAGIGGACWV ILMGFSIWLY WRRKKRKGLS NYAVTFQRGD GGLMSNGSRP GLLNAGDPSY PWLADSWPAT SLPVNNSNSG PNEIGNFGRG DVLPPVPGQG DKTATMLSDG AIYSSIDFTT KTSYNSSSQI TQATPYATTQ ILHSNSIHEL AVDLPDPQWK SSIQQKTDLM GFGYSLPDQN KGNNGGKGGK KKKNKNSSKP QKNNGSTWAN VPLPPPPVQP LPGTELEHYA VEQQENGYDS DSWCPPLPVQ TYLHQGLEDE LEEDDDRVPT PPVRGVASSP AISFGQQSTA TLTPSPREEM QPMLQAHLDE LTRAYQFDIA KQTWHIQSNN QPPQPPVPPL GYVSGALISD LETDVADDDA DDEEEALEIP RPLRALDQTP GSSMDNLDSS VTGKAFTSSQ RPRPTSPFST DSNTSAALSQ SQRPRPTKKH KGGRMDQQPA LPHRREGMTD EEALVPYSKP SFPSPGGHSS SGTASSKGST GPRKTEVLRA GHQRNASDLL DIGYMGSNSQ GQFTGEL
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Produktmerkmale:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human ROBO2 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Three step purification of membrane proteins expressed in baculovirus infected SF9 insect cells:

1. Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot.
2. The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot.
3. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Protein is endotoxin-free.

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Kommentare:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: ROBO2 (Roundabout, Axon Guidance Receptor, Homolog 2 (ROBO2))

Andere Bezeichnung: ROBO2 (ROBO2 Produkte)

Synonyme: SAX3 Protein, ROBO2 Protein, CG14347 Protein, CG14348 Protein, CG5481 Protein, CG5574 Protein, CT17326 Protein, D-Robo2 Protein, D-robo2 Protein, Dmel\\CG5481 Protein, Robo 2 Protein, Robo-2 Protein, Robo2 Protein, anon-EST:Liang-1.75 Protein, clone 1.75 Protein, dRobo-2 Protein, fus4 Protein, robo-2 Protein, robo2 Protein, unp1881 Protein, 2600013A04Rik Protein, 9430089E08Rik Protein, BB097918 Protein, D230004I22Rik Protein, mKIAA1568 Protein, sax3 Protein, roundabout guidance receptor 2 Protein, roundabout 2 Protein, roundabout, axon guidance receptor, homolog 2 (Drosophila) Protein, roundabout guidance receptor 2 S homeolog Protein, ROBO2 Protein, Robo2 Protein, robo2 Protein, robo2.S Protein

Hintergrund: Receptor for SLIT2, and probably SLIT1, which are thought to act as molecular guidance cue in cellular migration, including axonal navigation at the ventral midline of the neural tube and projection of axons to different regions during neuronal development.

Molekulargewicht: 150.0 kDa Including tag.

UniProt: Q9HCK4

Pathways: Regulation of Cell Size