Telefon:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@antikoerper-online.de

RALGAPB Protein (AA 1-1494) (His tag)

Crystallography grade RALGAPB Spezies: Human Wirt: Insektenzellen Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. Crys, ELISA, SDS, WB
Produktnummer ABIN3095052
  • Target Alle RALGAPB Produkte
    RALGAPB (Ral GTPase Activating Protein, beta Subunit (Non-Catalytic) (RALGAPB))
    Protein-Typ
    Recombinant
    Proteineigenschaft
    AA 1-1494
    Spezies
    Human
    Quelle
    • 1
    Insektenzellen
    Aufreinigungstag / Konjugat
    Dieses RALGAPB Protein ist gelabelt mit His tag.
    Applikation
    Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB)
    Sequenz
    MYSEWRSLHL VIQNDQGHTS VLHSYPESVG REVANAVVRP LGQVLGTPSV AGSENLLKTD KEVKWTMEVI CYGLTLPLDG ETVKYCVDVY TDWIMALVLP KDSIPLPVIK EPNQYVQTIL KHLQNLFVPR QEQGSSQIRL CLQVLRAIQK LARESSLMAR ETWEVLLLFL LQINDILLAP PTVQGGIAEN LAEKLIGVLF EVWLLACTRC FPTPPYWKTA KEMVANWRHH PAVVEQWSKV ICALTSRLLR FTYGPSFPAF KVPDEDASLI PPEMDNECVA QTWFRFLHML SNPVDLSNPA IISSTPKFQE QFLNVSGMPQ ELNQYPCLKH LPQIFFRAMR GISCLVDAFL GISRPRSDSA PPTPVNRLSM PQSAAVSTTP PHNRRHRAVT VNKATMKTST VSTAHASKVQ HQTSSTSPLS SPNQTSSEPR PLPAPRRPKV NSILNLFGSW LFDAAFVHCK LHNGINRDSS MTAITTQASM EFRRKGSQMS TDTMVSNPMF DASEFPDNYE AGRAEACGTL CRIFCSKKTG EEILPAYLSR FYMLLIQGLQ INDYVCHPVL ASVILNSPPL FCCDLKGIDV VVPYFISALE TILPDRELSK FKSYVNPTEL RRSSINILLS LLPLPHHFGT VKSEVVLEGK FSNDDSSSYD KPITFLSLKL RLVNILIGAL QTETDPNNTQ MILGAMLNIV QDSALLEAIG CQMEMGGGEN NLKSHSRTNS GISSASGGST EPTTPDSERP AQALLRDYAL NTDSAAGLLI RSIHLVTQRL NSQWRQDMSI SLAALELLSG LAKVKVMVDS GDRKRAISSV CTYIVYQCSR PAPLHSRDLH SMIVAAFQCL CVWLTEHPDM LDEKDCLKEV LEIVELGISG SKSKNNEQEV KYKGDKEPNP ASMRVKDAAE ATLTCIMQLL GAFPSPSGPA SPCSLVNETT LIKYSRLPTI NKHSFRYFVL DNSVILAMLE QPLGNEQNDF FPSVTVLVRG MSGRLAWAQQ LCLLPRGAKA NQKLFVPEPR PVPKNDVGFK YSVKHRPFPE EVDKIPFVKA DLSIPDLHEI VTEELEERHE KLRSGMAQQI AYEIHLEQQS EEELQKRSFP DPVTDCKPPP PAQEFQTARL FLSHFGFLSL EALKEPANSR LPPHLIALDS TIPGFFDDIG YLDLLPCRPF DTVFIFYMKP GQKTNQEILK NVESSRTVQP HFLEFLLSLG WSVDVGRHPG WTGHVSTSWS INCCDDGEGS QQEVISSEDI GASIFNGQKK VLYYADALTE IAFVVPSPVE SLTDSLESNI SDQDSDSNMD LMPGILKQPS LTLELFPNHT DNLNSSQRLS PSSRMRKLPQ GRPVPPLGPE TRVSVVWVER YDDIENFPLS ELMTEISTGV ETTANSSTSL RSTTLEKEVP VIFIHPLNTG LFRIKIQGAT GKFNMVIPLV DGMIVSRRAL GFLVRQTVIN ICRRKRLESD SYSPPHVRRK QKITDIVNKY RNKQLEPEFY TSLFQEVGLK NCSS
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Produktmerkmale
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Human RALGAPB Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Aufreinigung
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Reinheit
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterilität
    0.22 μm filtered
    Endotoxin-Niveau
    Protein is endotoxin free.
    Güteklasse
    Crystallography grade
  • Applikationshinweise
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Kommentare

    In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handhabung
    Avoid repeated freeze-thaw cycles.
    Lagerung
    -80 °C
    Informationen zur Lagerung
    Store at -80°C.
    Haltbarkeit
    Unlimited (if stored properly)
  • Target
    RALGAPB (Ral GTPase Activating Protein, beta Subunit (Non-Catalytic) (RALGAPB))
    Andere Bezeichnung
    RALGAPB (RALGAPB Produkte)
    Synonyme
    KIAA1219 Protein, RalGAPbeta Protein, dJ1100H13.1 Protein, 9330195P16 Protein, AI507211 Protein, B230339M05Rik Protein, mKIAA1219 Protein, RGD1306861 Protein, p170 Protein, Ral GTPase activating protein non-catalytic beta subunit Protein, Ral GTPase activating protein, beta subunit (non-catalytic) Protein, RALGAPB Protein, Ralgapb Protein
    Hintergrund
    Non-catalytic subunit of the heterodimeric RalGAP1 and RalGAP2 complexes which act as GTPase activators for the Ras-like small GTPases RALA and RALB. {ECO:0000250}.
    Molekulargewicht
    167.8 kDa Including tag.
    UniProt
    Q86X10
Sie sind hier:
Kundenservice