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RNF2 Protein (AA 2-336) (His tag)

Crystallography grade RNF2 Spezies: Human Wirt: Insektenzellen Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, Crys, SDS
Produktnummer ABIN3094999
  • Target Alle RNF2 Proteine anzeigen
    RNF2 (Ring Finger Protein 2 (RNF2))
    Protein-Typ
    Recombinant
    Proteineigenschaft
    AA 2-336
    Spezies
    • 3
    • 1
    • 1
    • 1
    Human
    Quelle
    • 2
    • 2
    • 1
    • 1
    Insektenzellen
    Aufreinigungstag / Konjugat
    Dieses RNF2 Protein ist gelabelt mit His tag.
    Applikation
    ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)
    Sequenz
    SQAVQTNGTQ PLSKTWELSL YELQRTPQEA ITDGLEIVVS PRSLHSELMC PICLDMLKNT MTTKECLHRF CADCIITALR SGNKECPTCR KKLVSKRSLR PDPNFDALIS KIYPSRDEYE AHQERVLARI NKHNNQQALS HSIEEGLKIQ AMNRLQRGKK QQIENGSGAE DNGDSSHCSN ASTHSNQEAG PSNKRTKTSD DSGLELDNNN AAMAIDPVMD GASEIELVFR PHPTLMEKDD SAQTRYIKTS GNATVDHLSK YLAVRLALEE LRSKGESNQM NLDTASEKQY TIYIATASGQ FTVLNGSFSL ELVSEKYWKV NKPMELYYAP TKEHK
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Produktmerkmale
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Human RNF2 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Aufreinigung
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Reinheit
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterilität
    0.22 μm filtered
    Endotoxin-Niveau
    Protein is endotoxin free.
    Güteklasse
    Crystallography grade
    Top Product
    Discover our top product RNF2 Protein
  • Applikationshinweise
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Kommentare

    In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handhabung
    Avoid repeated freeze-thaw cycles.
    Lagerung
    -80 °C
    Informationen zur Lagerung
    Store at -80°C.
    Haltbarkeit
    Unlimited (if stored properly)
  • Target
    RNF2 (Ring Finger Protein 2 (RNF2))
    Andere Bezeichnung
    RNF2 (RNF2 Produkte)
    Synonyme
    BAP-1 Protein, BAP1 Protein, DING Protein, HIPI3 Protein, RING1B Protein, RING2 Protein, AI326319 Protein, AI450156 Protein, AU019207 Protein, Ring1B Protein, dinG Protein, fa19h05 Protein, ring1b Protein, wu:fa19h05 Protein, wz4556 Protein, bap-1 Protein, bap1 Protein, ding Protein, hipi3 Protein, ring2 Protein, ring finger protein 2 Protein, ring finger protein 2 L homeolog Protein, RNF2 Protein, Rnf2 Protein, rnf2 Protein, rnf2.L Protein
    Hintergrund
    E3 ubiquitin-protein ligase that mediates monoubiquitination of 'Lys-119' of histone H2A (H2AK119Ub), thereby playing a central role in histone code and gene regulation. H2AK119Ub gives a specific tag for epigenetic transcriptional repression and participates in X chromosome inactivation of female mammals. May be involved in the initiation of both imprinted and random X inactivation. Essential component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones, rendering chromatin heritably changed in its expressibility. E3 ubiquitin-protein ligase activity is enhanced by BMI1/PCGF4. Acts as the main E3 ubiquitin ligase on histone H2A of the PRC1 complex, while RING1 may rather act as a modulator of RNF2/RING2 activity. In resting B- and T-lymphocytes, interaction with AURKB leads to block its activity, thereby maintaining transcription in resting lymphocytes. {ECO:0000269|PubMed:11513855, ECO:0000269|PubMed:15386022, ECO:0000269|PubMed:16359901, ECO:0000269|PubMed:16714294, ECO:0000269|PubMed:20696397}.
    Molekulargewicht
    38.5 kDa Including tag.
    UniProt
    Q99496
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