PZP Protein (AA 26-1482) (His tag)

Produktnummer ABIN3094904

Produktdetails

Target: PZP (Pregnancy Zone Protein (PZP))

Protein-Typ: Recombinant

Proteineigenschaft: AA 26-1482

Spezies: Human

Quelle: Insektenzellen

Aufreinigungstag / Konjugat: Dieses PZP Protein ist gelabelt mit His tag.

Applikation: SDS-PAGE (SDS), Western Blotting (WB), ELISA, Crystallization (Crys)

Sequenz: TEPQYMVLVP SLLHTEAPKK GCVLLSHLNE TVTVSASLES GRENRSLFTD LVAEKDLFHC VSFTLPRISA SSEVAFLSIQ IKGPTQDFRK RNTVLVLNTQ SLVFVQTDKP MYKPGQTVRF RVVSVDENFR PRNELIPLIY LENPRRNRIA QWQSLKLEAG INQLSFPLSS EPIQGSYRVV VQTESGGRIQ HPFTVEEFVL PKFEVKVQVP KIISIMDEKV NITVCGEYTY GKPVPGLATV SLCRKLSRVL NCDKQEVCEE FSQQLNSNGC ITQQVHTKML QITNTGFEMK LRVEARIREE GTDLEVTANR ISEITNIVSK LKFVKVDSHF RQGIPFFAQV LLVDGKGVPI PNKLFFISVN DANYYSNATT NEQGLAQFSI NTTSISVNKL FVRVFTVHPN LCFHYSWVAE DHQGAQHTAN RVFSLSGSYI HLEPVAGTLP CGHTETITAH YTLNRQAMGE LSELSFHYLI MAKGVIVRSG THTLPVESGD MKGSFALSFP VESDVAPIAR MFIFAILPDG EVVGDSEKFE IENCLANKVD LSFSPAQSPP ASHAHLQVAA APQSLCALRA VDQSVLLMKP EAELSVSSVY NLLTVKDLTN FPDNVDQQEE EQGHCPRPFF IHNGAIYVPL SSNEADIYSF LKGMGLKVFT NSKIRKPKSC SVIPSVSAGA VGQGYYGAGL GVVERPYVPQ LGTYNVIPLN NEQSSGPVPE TVRSYFPETW IWELVAVNSS GVAEVGVTVP DTITEWKAGA FCLSEDAGLG ISSTASLRAF QPFFVELTMP YSVIRGEVFT LKATVLNYLP KCIRVSVQLK ASPAFLASQN TKGEESYCIC GNERQTLSWT VTPKTLGNVN FSVSAEAMQS LELCGNEVVE VPEIKRKDTV IKTLLVEAEG IEQEKTFSSM TCASGANVSE QLSLKLPSNV VKESARASFS VLGDILGSAM QNIQNLLQMP YGCGEQNMVL FAPNIYVLNY LNETQQLTQE IKAKAVGYLI TGYQRQLNYK HQDGSYSTFG ERYGRNQGNT WLTAFVLKTF AQARSYIFID EAHITQSLTW LSQMQKDNGC FRSSGSLLNN AIKGGVEDEA TLSAYVTIAL LEIPLPVTNP IVRNALFCLE SAWNVAKEGT HGSHVYTKAL LAYAFSLLGK QNQNREILNS LDKEAVKEDN LVHWERPQRP KAPVGHLYQT QAPSAEVEMT SYVLLAYLTA QPAPTSGDLT SATNIVKWIM KQQNAQGGFS STQDTVVALH ALSRYGAATF TRTEKTAQVT VQDSQTFSTN FQVDNNNLLL LQQISLPELP GEYVITVTGE RCVYLQTSMK YNILPEKEDS PFALKVQTVP QTCDGHKAHT SFQISLTISY TGNRPASNMV IVDVKMVSGF IPLKPTVKML ERSSSVSRTE VSNNHVLIYV EQVTNQTLSF SFMVLQDIPV GDLKPAIVKV YDYYETDESV VAEYIAPCST DTEHGNV
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Produktmerkmale:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human PZP Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Protein is endotoxin free.

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Kommentare:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: PZP (Pregnancy Zone Protein (PZP))

Andere Bezeichnung: PZP (PZP Produkte)

Synonyme: A1m Protein, pregnancy zone protein Protein, pregnancy-zone protein Protein, CpipJ_CPIJ000348 Protein, Pzp Protein, PZP Protein

Hintergrund: Is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase.

Molekulargewicht: 162.0 kDa Including tag.

UniProt: P20742