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RAB3GAP1 Protein (AA 2-981) (His tag)

Crystallography grade RAB3GAP1 Spezies: Human Wirt: Insektenzellen Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, SDS, WB, Crys
Produktnummer ABIN3094899
  • Target Alle RAB3GAP1 Proteine anzeigen
    RAB3GAP1 (RAB3 GTPase Activating Protein Subunit 1 (Catalytic) (RAB3GAP1))
    Protein-Typ
    Recombinant
    Proteineigenschaft
    AA 2-981
    Spezies
    • 1
    • 1
    • 1
    Human
    Quelle
    • 1
    • 1
    • 1
    Insektenzellen
    Aufreinigungstag / Konjugat
    Dieses RAB3GAP1 Protein ist gelabelt mit His tag.
    Applikation
    ELISA, SDS-PAGE (SDS), Western Blotting (WB), Crystallization (Crys)
    Sequenz
    AADSEPESEV FEITDFTTAS EWERFISKVE EVLNDWKLIG NSLGKPLEKG IFTSGTWEEK SDEISFADFK FSVTHHYLVQ ESTDKEGKDE LLEDVVPQSM QDLLGMNNDF PPRAHCLVRW YGLREFVVIA PAAHSDAVLS ESKCNLLLSS VSIALGNTGC QVPLFVQIHH KWRRMYVGEC QGPGVRTDFE MVHLRKVPNQ YTHLSGLLDI FKSKIGCPLT PLPPVSIAIR FTYVLQDWQQ YFWPQQPPDI DALVGGEVGG LEFGKLPFGA CEDPISELHL ATTWPHLTEG IIVDNDVYSD LDPIQAPHWS VRVRKAENPQ CLLGDFVTEF FKICRRKEST DEILGRSAFE EEGKETADIT HALSKLTEPA SVPIHKLSVS NMVHTAKKKI RKHRGVEESP LNNDVLNTIL LFLFPDAVSE KPLDGTTSTD NNNPPSESED YNLYNQFKSA PSDSLTYKLA LCLCMINFYH GGLKGVAHLW QEFVLEMRFR WENNFLIPGL ASGPPDLRCC LLHQKLQMLN CCIERKKARD EGKKTSASDV TNIYPGDAGK AGDQLVPDNL KETDKEKGEV GKSWDSWSDS EEEFFECLSD TEELKGNGQE SGKKGGPKEM ANLRPEGRLY QHGKLTLLHN GEPLYIPVTQ EPAPMTEDLL EEQSEVLAKL GTSAEGAHLR ARMQSACLLS DMESFKAANP GCSLEDFVRW YSPRDYIEEE VIDEKGNVVL KGELSARMKI PSNMWVEAWE TAKPIPARRQ RRLFDDTREA EKVLHYLAIQ KPADLARHLL PCVIHAAVLK VKEEESLENI SSVKKIIKQI ISHSSKVLHF PNPEDKKLEE IIHQITNVEA LIARARSLKA KFGTEKCEQE EEKEDLERFV SCLLEQPEVL VTGAGRGHAG RIIHKLFVNA QRAAAMTPPE EELKRMGSPE ERRQNSVSDF PPPAGREFIL RTTVPRPAPY SKALPQRMYS VLTKEDFRLA GAFSSDTSFF
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Produktmerkmale
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Human RAB3GAP1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Aufreinigung
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Reinheit
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterilität
    0.22 μm filtered
    Endotoxin-Niveau
    Protein is endotoxin free.
    Güteklasse
    Crystallography grade
  • Applikationshinweise
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Kommentare

    In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handhabung
    Avoid repeated freeze-thaw cycles.
    Lagerung
    -80 °C
    Informationen zur Lagerung
    Store at -80°C.
    Haltbarkeit
    Unlimited (if stored properly)
  • Target
    RAB3GAP1 (RAB3 GTPase Activating Protein Subunit 1 (Catalytic) (RAB3GAP1))
    Andere Bezeichnung
    RAB3GAP1 (RAB3GAP1 Produkte)
    Synonyme
    1700003B17Rik Protein, 4732493F09Rik Protein, AL117896 Protein, Rab3gap Protein, p130 Protein, P130 Protein, RAB3GAP Protein, RAB3GAP130 Protein, WARBM1 Protein, RAB3 GTPase activating protein subunit 1 Protein, RAB3 GTPase activating protein catalytic subunit 1 Protein, Rab3gap1 Protein, RAB3GAP1 Protein
    Hintergrund
    Probable catalytic subunit of a GTPase activating protein that has specificity for Rab3 subfamily (RAB3A, RAB3B, RAB3C and RAB3D). Rab3 proteins are involved in regulated exocytosis of neurotransmitters and hormones. Specifically converts active Rab3-GTP to the inactive form Rab3-GDP. Required for normal eye and brain development. May participate in neurodevelopmental processes such as proliferation, migration and differentiation before synapse formation, and non-synaptic vesicular release of neurotransmitters. {ECO:0000269|PubMed:10859313, ECO:0000269|PubMed:9030515}.
    Molekulargewicht
    111.3 kDa Including tag.
    UniProt
    Q15042
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