PTPRF Protein (AA 30-1263) (His tag)

Produktnummer ABIN3094810

Produktdetails

Target: PTPRF (Protein Tyrosine Phosphatase Receptor Type F (PTPRF))

Protein-Typ: Recombinant

Proteineigenschaft: AA 30-1263

Spezies: Human

Quelle: Insektenzellen

Aufreinigungstag / Konjugat: Dieses PTPRF Protein ist gelabelt mit His tag.

Applikation: Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)

Sequenz: DSKPVFIKVP EDQTGLSGGV ASFVCQATGE PKPRITWMKK GKKVSSQRFE VIEFDDGAGS VLRIQPLRVQ RDEAIYECTA TNSLGEINTS AKLSVLEEEQ LPPGFPSIDM GPQLKVVEKA RTATMLCAAG GNPDPEISWF KDFLPVDPAT SNGRIKQLRS GALQIESSEE SDQGKYECVA TNSAGTRYSA PANLYVRVRR VAPRFSIPPS SQEVMPGGSV NLTCVAVGAP MPYVKWMMGA EELTKEDEMP VGRNVLELSN VVRSANYTCV AISSLGMIEA TAQVTVKALP KPPIDLVVTE TTATSVTLTW DSGNSEPVTY YGIQYRAAGT EGPFQEVDGV ATTRYSIGGL SPFSEYAFRV LAVNSIGRGP PSEAVRARTG EQAPSSPPRR VQARMLSAST MLVQWEPPEE PNGLVRGYRV YYTPDSRRPP NAWHKHNTDA GLLTTVGSLL PGITYSLRVL AFTAVGDGPP SPTIQVKTQQ GVPAQPADFQ AEVESDTRIQ LSWLLPPQER IIMYELVYWA AEDEDQQHKV TFDPTSSYTL EDLKPDTLYR FQLAARSDMG VGVFTPTIEA RTAQSTPSAP PQKVMCVSMG STTVRVSWVP PPADSRNGVI TQYSVAYEAV DGEDRGRHVV DGISREHSSW DLVGLEKWTE YRVWVRAHTD VGPGPESSPV LVRTDEDVPS GPPRKVEVEP LNSTAVHVYW KLPVPSKQHG QIRGYQVTYV RLENGEPRGL PIIQDVMLAE AQWRPEESED YETTISGLTP ETTYSVTVAA YTTKGDGARS KPKIVTTTGA VPGRPTMMIS TTAMNTALLQ WHPPKELPGE LLGYRLQYCR ADEARPNTID FGKDDQHFTV TGLHKGTTYI FRLAAKNRAG LGEEFEKEIR TPEDLPSGFP QNLHVTGLTT STTELAWDPP VLAERNGRII SYTVVFRDIN SQQELQNITT DTRFTLTGLK PDTTYDIKVR AWTSKGSGPL SPSIQSRTMP VEQVFAKNFR VAAAMKTSVL LSWEVPDSYK SAVPFKILYN GQSVEVDGHS MRKLIADLQP NTEYSFVLMN RGSSAGGLQH LVSIRTAPDL LPHKPLPASA YIEDGRFDLS MPHVQDPSLV RWFYIVVVPI DRVGGSMLTP RWSTPEELEL DELLEAIEQG GEEQRRRRRQ AERLKPYVAA QLDVLPETFT LGDKKNYRGF YNRPLSPDLS YQCFVLASLK EPMDQKRYAS SPYSDEIVVQ VTPAQQQEEP EMLW
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Produktmerkmale:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human PTPRF Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Protein is endotoxin free.

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Kommentare:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: PTPRF (Protein Tyrosine Phosphatase Receptor Type F (PTPRF))

Andere Bezeichnung: PTPRF (PTPRF Produkte)

Synonyme: LAR Protein, AA591035 Protein, LARS Protein, RPTP-LAR Protein, LARFN5C Protein, zf-LAR Protein, protein tyrosine phosphatase, receptor type F Protein, protein tyrosine phosphatase, receptor type, F Protein, protein tyrosine phosphatase, receptor type, f, a Protein, PTPRF Protein, Ptprf Protein, ptprfa Protein

Hintergrund: Possible cell adhesion receptor. It possesses an intrinsic protein tyrosine phosphatase activity (PTPase) and dephosphorylates EPHA2 regulating its activity., The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one.

Molekulargewicht: 137.4 kDa Including tag.

UniProt: P10586

Pathways: EGFR Signaling Pathway