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PER3 Protein (AA 1-1201) (His tag)

Crystallography grade PER3 Spezies: Human Wirt: Insektenzellen Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, Crys, SDS
Produktnummer ABIN3094556
  • Target Alle PER3 Proteine anzeigen
    PER3 (Period Homolog 3 (PER3))
    Protein-Typ
    Recombinant
    Proteineigenschaft
    AA 1-1201
    Spezies
    • 2
    • 1
    Human
    Quelle
    • 2
    • 1
    Insektenzellen
    Aufreinigungstag / Konjugat
    Dieses PER3 Protein ist gelabelt mit His tag.
    Applikation
    ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)
    Sequenz
    MPRGEAPGPG RRGAKDEALG EESGERWSPE FHLQRKLADS SHSEQQDRNR VSEELIMVVQ EMKKYFPSER RNKPSTLDAL NYALRCVHSV QANSEFFQIL SQNGAPQADV SMYSLEELAT IASEHTSKNT DTFVAVFSFL SGRLVHISEQ AALILNRKKD VLASSHFVDL LAPQDMRVFY AHTARAQLPF WNNWTQRAAR YECAPVKPFF CRIRGGEDRK QEKCHSPFRI IPYLIHVHHP AQPELESEPC CLTVVEKIHS GYEAPRIPVN KRIFTTTHTP GCVFLEVDEK AVPLLGYLPQ DLIGTSILSY LHPEDRSLMV AIHQKVLKYA GHPPFEHSPI RFCTQNGDYI ILDSSWSSFV NPWSRKISFI IGRHKVRTSP LNEDVFATKI KKMNDNDKDI TELQEQIYKL LLQPVHVSVS SGYGSLGSSG SQEQLVSIAS SSEASGHRVE ETKAEQMTLQ QVYASVNKIK NLGQQLYIES MTKSSFKPVT GTRTEPNGGG ECKTFTSFHQ TLKNNSVYTE PCEDLRNDEH SPSYQQINCI DSVIRYLKSY NIPALKRKCI SCTNTTSSSS EEDKQNHKAD DVQALQAGLQ IPAIPKSEMP TNGRSIDTGG GAPQILSTAM LSLGSGISQC GYSSTIVHVP PPETARDATL FCEPWTLNMQ PAPLTSEEFK HVGLTAAVLS AHTQKEEQNY VDKFREKILS SPYSSYLQQE SRSKAKYSYF QGDSTSKQTR SAGCRKGKHK RKKLPEPPDS SSSNTGSGPR RGAHQNAQPC CPSAASSPHT SSPTFPPAAM VPSQAPYLVP AFPLPAATSP GREYAAPGTA PEGLHGLPLS EGLQPYPAFP FPYLDTFMTV FLPDPPVCPL LSPSFLPCPF LGATASSAIS PSMSSAMSPT LDPPPSVTSQ RREEEKWEAQ SEGHPFITSR SSSPLQLNLL QEEMPRPSES PDQMRRNTCP QTEYCVTGNN GSESSPATTG ALSTGSPPRE NPSHPTASAL STGSPPMKNP SHPTASALST GSPPMKNPSH PTASTLSMGL PPSRTPSHPT ATVLSTGSPP SESPSRTGSA ASGSSDSSIY LTSSVYSSKI SQNGQQSQDV QKKETFPNVA EEPIWRMIRQ TPERILMTYQ VPERVKEVVL KEDLEKLESM RQQQPQFSHG QKEELAKVYN WIQSQTVTQE IDIQACVTCE NEDSADGAAT SCGQVLVEDS C
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Produktmerkmale
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Human PER3 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Aufreinigung
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Reinheit
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterilität
    0.22 μm filtered
    Endotoxin-Niveau
    Protein is endotoxin free.
    Güteklasse
    Crystallography grade
    Top Product
    Discover our top product PER3 Protein
  • Applikationshinweise
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Kommentare

    In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handhabung
    Avoid repeated freeze-thaw cycles.
    Lagerung
    -80 °C
    Informationen zur Lagerung
    Store at -80°C.
    Haltbarkeit
    Unlimited (if stored properly)
  • Target
    PER3 (Period Homolog 3 (PER3))
    Andere Bezeichnung
    PER3 (PER3 Produkte)
    Synonyme
    period3 Protein, rper3 Protein, GIG13 Protein, 2810049O06Rik Protein, mPer3 Protein, period circadian clock 3 Protein, period circadian regulator 3 Protein, Per3 Protein, PER3 Protein
    Hintergrund
    Originally described as a core component of the circadian clock. The circadian clock, an internal time-keeping system, regulates various physiological processes through the generation of approximately 24 hour circadian rhythms in gene expression, which are translated into rhythms in metabolism and behavior. It is derived from the Latin roots 'circa' (about) and 'diem' (day) and acts as an important regulator of a wide array of physiological functions including metabolism, sleep, body temperature, blood pressure, endocrine, immune, cardiovascular, and renal function. Consists of two major components: the central clock, residing in the suprachiasmatic nucleus (SCN) of the brain, and the peripheral clocks that are present in nearly every tissue and organ system. Both the central and peripheral clocks can be reset by environmental cues, also known as Zeitgebers (German for 'timegivers'). The predominant Zeitgeber for the central clock is light, which is sensed by retina and signals directly to the SCN. The central clock entrains the peripheral clocks through neuronal and hormonal signals, body temperature and feeding-related cues, aligning all clocks with the external light/dark cycle. Circadian rhythms allow an organism to achieve temporal homeostasis with its environment at the molecular level by regulating gene expression to create a peak of protein expression once every 24 hours to control when a particular physiological process is most active with respect to the solar day. Transcription and translation of core clock components (CLOCK, NPAS2, ARNTL/BMAL1, ARNTL2/BMAL2, PER1, PER2, PER3, CRY1 and CRY2) plays a critical role in rhythm generation, whereas delays imposed by post-translational modifications (PTMs) are important for determining the period (tau) of the rhythms (tau refers to the period of a rhythm and is the length, in time, of one complete cycle). A diurnal rhythm is synchronized with the day/night cycle, while the ultradian and infradian rhythms have a period shorter and longer than 24 hours, respectively. Disruptions in the circadian rhythms contribute to the pathology of cardiovascular diseases, cancer, metabolic syndromes and aging. A transcription/translation feedback loop (TTFL) forms the core of the molecular circadian clock mechanism. Transcription factors, CLOCK or NPAS2 and ARNTL/BMAL1 or ARNTL2/BMAL2, form the positive limb of the feedback loop, act in the form of a heterodimer and activate the transcription of core clock genes and clock-controlled genes (involved in key metabolic processes), harboring E-box elements (5'-CACGTG-3') within their promoters. The core clock genes: PER1/2/3 and CRY1/2 which are transcriptional repressors form the negative limb of the feedback loop and interact with the CLOCK|NPAS2-ARNTL/BMAL1|ARNTL2/BMAL2 heterodimer inhibiting its activity and thereby negatively regulating their own expression. This heterodimer also activates nuclear receptors NR1D1, NR1D2, RORA, RORB and RORG, which form a second feedback loop and which activate and repress ARNTL/BMAL1 transcription, respectively. Has a redundant role with the other PER proteins PER1 and PER2 and is not essential for the circadian rhythms maintenance. In contrast, plays an important role in sleep-wake timing and sleep homeostasis probably through the transcriptional regulation of sleep homeostasis-related genes, without influencing circadian parameters. Can bind heme. {ECO:0000269|PubMed:17346965, ECO:0000269|PubMed:19716732, ECO:0000269|PubMed:24439663, ECO:0000269|PubMed:24577121}.
    Molekulargewicht
    132.8 kDa Including tag.
    UniProt
    P56645
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