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PIDD Protein (AA 2-910) (His tag)

Crystallography grade PIDD Spezies: Human Wirt: Insektenzellen Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. Crys, ELISA, SDS, WB
Produktnummer ABIN3094536
  • Target Alle PIDD Proteine anzeigen
    PIDD (P53-Induced Death Domain Protein (PIDD))
    Protein-Typ
    Recombinant
    Proteineigenschaft
    AA 2-910
    Spezies
    Human
    Quelle
    • 1
    Insektenzellen
    Aufreinigungstag / Konjugat
    Dieses PIDD Protein ist gelabelt mit His tag.
    Applikation
    Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB)
    Sequenz
    AATVEGPELE AAAAAGDASE DSDAGSRALP FLGGNRLSLD LYPGGCQQLL HLCVQQPLQL LQVEFLRLST HEDPQLLEAT LAQLPQSLSC LRSLVLKGGQ RRDTLGACLR GALTNLPAGL SGLAHLAHLD LSFNSLETLP ACVLQMRGLG ALLLSHNCLS ELPEALGALP ALTFLTVTHN RLQTLPPALG ALSTLQRLDL SQNLLDTLPP EIGGLGSLLE LNLASNRLQS LPASLAGLRS LRLLVLHSNL LASVPADLAR LPLLTRLDLR DNQLRDLPPE LLDAPFVRLQ GNPLGEASPD APSSPVAALI PEMPRLFLTS DLDSFPVTPQ GCSVTLACGV RLQFPAGATA TPITIRYRLL LPEPGLVPLG PHDALLSHVL ELQPHGVAFQ QDVGLWLLFT PPQARRCREV VVRTRNDNSW GDLETYLEEE APQRLWAHCQ VPHFSWFLVV SRPVSNACLV PPEGTLLCSS GHPGVKVIFP PGATEEPRRV SMQVVRMAGR ELQALLGEPE AAVSPLLCLS QSGPPSFLQP VTVQLPLPSG ITGLSLDRSR LHLLYWAPPA ATWDDITAQV VLELTHLYAR FQVTHFSWYW LWYTTKNCVG GLARKAWERL RLHRVNLIAL QRRRDPEQVL LQCLPRNKVD ATLRRLLERY RGPEPSDTVE MFEGEEFFAA FERGIDVDAD RPDCVEGRIC FVFYSHLKNV KEVYVTTTLD REAQAVRGQV SFYRGAVPVR VPEEAEAARQ RKGADALWMA TLPIKLPRLR GSEGPRRGAG LSLAPLNLGD AETGFLTQSN LLSVAGRLGL DWPAVALHLG VSYREVQRIR HEFRDDLDEQ IRHMLFSWAE RQAGQPGAVG LLVQALEQSD RQDVAEEVRA VLELGRRKYQ DSIRRMGLAP KDPALPGSSA PQPPEPAQA
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Produktmerkmale
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Human PIDD1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Aufreinigung
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Reinheit
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterilität
    0.22 μm filtered
    Endotoxin-Niveau
    Protein is endotoxin free.
    Güteklasse
    Crystallography grade
  • Applikationshinweise
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Kommentare

    In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handhabung
    Avoid repeated freeze-thaw cycles.
    Lagerung
    -80 °C
    Informationen zur Lagerung
    Store at -80°C.
    Haltbarkeit
    Unlimited (if stored properly)
  • Target
    PIDD (P53-Induced Death Domain Protein (PIDD))
    Andere Bezeichnung
    PIDD1 (PIDD Produkte)
    Synonyme
    1200011D09Rik Protein, AU042446 Protein, Pidd Protein, LRDD Protein, Lrdd Protein, p53 induced death domain protein 1 Protein, p53-induced death domain protein 1 Protein, Pidd1 Protein, PIDD1 Protein
    Hintergrund
    Promotes apoptosis downstream of the tumor suppressor as component of the DNA damage/stress response pathway that connects p53/TP53 to apoptosis. Associates with NEMO/IKBKG and RIP1 and enhances sumoylation and ubiquitination of NEMO/IKBKG which is important for activation of the transcription factor NF-kappa-B. Associates with CASP2/caspase-2 and CRADD/RAIDD, and induces activation of CASP2 which an important regulator in apoptotic pathways. {ECO:0000269|PubMed:10973264, ECO:0000269|PubMed:15073321, ECO:0000269|PubMed:16360037}.
    Molekulargewicht
    100.5 kDa Including tag.
    UniProt
    Q9HB75
    Pathways
    p53 Signalweg, Caspase Kaskade in der Apoptose, Positive Regulation of Endopeptidase Activity
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