PCF11 Protein (AA 2-1555) (His tag)

Produktnummer ABIN3094482

Produktdetails

Target: PCF11 (PCF11, Cleavage and Polyadenylation Factor Subunit (PCF11))

Protein-Typ: Recombinant

Proteineigenschaft: AA 2-1555

Spezies: Human

Quelle: Insektenzellen

Aufreinigungstag / Konjugat: Dieses PCF11 Protein ist gelabelt mit His tag.

Applikation: ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)

Sequenz: SEQTPAEAGA AGAREDACRD YQSSLEDLTF NSKPHINMLT ILAEENLPFA KEIVSLIEAQ TAKAPSSEKL PVMYLMDSIV KNVGREYLTA FTKNLVATFI CVFEKVDENT RKSLFKLRST WDEIFPLKKL YALDVRVNSL DPAWPIKPLP PNVNTSSIHV NPKFLNKSPE EPSTPGTVVS SPSISTPPIV PDIQKNLTQE QLIRQQLLAK QKQLLELQQK KLELELEQAK AQLAVSLSVQ QETSNLGPGS APSKLHVSQI PPMAVKAPHQ VPVQSEKSRP GPSLQIQDLK GTNRDPRLNR ISQHSHGKDQ SHRKEFLMNT LNQSDTKTSK TIPSEKLNSS KQEKSKSGEK ITKKELDQLD SKSKSKSKSP SPLKNKLSHT KDLKNQESES MRLSDMNKRD PRLKKHLQDK TDGKDDDVKE KRKTAEKKDK DEHMKSSEHR LAGSRNKIIN GIVQKQDTIT EESEKQGTKP GRSSTRKRSR SRSPKSRSPI IHSPKRRDRR SPKRRQRSMS PTSTPKAGKI RQSGAKQSHM EEFTPPSRED RNAKRSTKQD IRDPRRMKKT EEERPQETTN QHSTKSGTEP KENVENWQSS KSAKRWKSGW EENKSLQQVD EHSKPPHLRH RESWSSTKGI LSPRAPKQQQ HRLSVDANLQ IPKELTLASK RELLQKTSER LASGEITQDD FLVVVHQIRQ LFQYQEGVRE EQRSPFNDRF PLKRPRYEDS DKPFVDSPAS RFAGLDTNQR LTALAEDRPL FDGPSRPSVA RDGPTKMIFE GPNKLSPRID GPPTPASLRF DGSPGQMGGG GPLRFEGPQG QLGGGCPLRF EGPPGPVGTP LRFEGPIGQA GGGGFRFEGS PGLRFEGSPG GLRFEGPGGQ PVGGLRFEGH RGQPVGGLRF EGPHGQPVGG LRFDNPRGQP VGGLRFEGGH GPSGAAIRFD GPHGQPGGGI RFEGPLLQQG VGMRFEGPHG QSVAGLRFEG QHNQLGGNLR FEGPHGQPGV GIRFEGPLVQ QGGGMRFEGP SVPGGGLRIE GPLGQGGPRF EGCHALRFDG QPGQPSLLPR FDGLHGQPGP RFERTPGQPG PQRFDGPPGQ QVQPRFDGVP QRFDGPQHQQ ASRFDIPLGL QGTRFDNHPS QRLESVSFNQ TGPYNDPPGN AFNAPSQGLQ FQRHEQIFDS PQGPNFNGPH GPGNQSFSNP LNRASGHYFD EKNLQSSQFG NFGNIPAPMT VGNIQASQQV LSGVAQPVAF GQGQQFLPVH PQNPGFVQNP SGALPKAYPD NHLSQVDVNE LFSKLLKTGI LKLSQTDSAT TQVSEVTAQP PPEEEEDQNE DQDVPDLTNF TVEELKQRYD SVINRLYTGI QCYSCGMRFT TSQTDVYADH LDWHYRQNRT EKDVSRKVTH RRWYYSLTDW IEFEEIADLE ERAKSQFFEK VHEEVVLKTQ EAAKEKEFQS VPAGPAGAVE SCEICQEQFE QYWDEEEEEW HLKNAIRVDG KIYHPSCYED YQNTSSFDCT PSPSKTPVEN PLNIMLNIVK NELQEPCDSP KVKEERIDTP PACTEESIAT PSEIKTENDT VESV
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Produktmerkmale:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human PCF11 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Protein is endotoxin free.

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Kommentare:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: PCF11 (PCF11, Cleavage and Polyadenylation Factor Subunit (PCF11))

Andere Bezeichnung: PCF11 (PCF11 Produkte)

Synonyme: 2500001H09Rik Protein, 5730417B17Rik Protein, C77803 Protein, pcf11 Protein, im:6912257 Protein, im:7138745 Protein, wu:fc47a05 Protein, zgc:175013 Protein, zgc:92226 Protein, PCF11 cleavage and polyadenylation factor subunit Protein, PCF11 cleavage and polyadenylation factor subunit S homeolog Protein, PCF11 Protein, Pcf11 Protein, pcf11.S Protein, pcf11 Protein

Hintergrund: Component of pre-mRNA cleavage complex II.

Molekulargewicht: 173.9 kDa Including tag.

UniProt: O94913