PCDHGA12 Protein (AA 30-932) (rho-1D4 tag)

Produktnummer ABIN3094472

Produktdetails

Target: PCDHGA12 (Protocadherin gamma 12 (PCDHGA12))

Protein-Typ: Recombinant

Proteineigenschaft: AA 30-932

Spezies: Human

Quelle: Insektenzellen

Aufreinigungstag / Konjugat: Dieses PCDHGA12 Protein ist gelabelt mit rho-1D4 tag.

Applikation: ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)

Sequenz: QIRYSVPEEL EKGSRVGDIS RDLGLEPREL AERGVRIIPR GRTQLFALNP RSGSLVTAGR IDREELCMGA IKCQLNLDIL MEDKVKIYGV EVEVRDINDN APYFRESELE IKISENAATE MRFPLPHAWD PDIGKNSLQS YELSPNTHFS LIVQNGADGS KYPELVLKRA LDREEKAAHH LVLTASDGGD PVRTGTARIR VMVLDANDNA PAFAQPEYRA SVPENLALGT QLLVVNATDP DEGVNAEVRY SFRYVDDKAA QVFKLDCNSG TISTIGELDH EESGFYQMEV QAMDNAGYSA RAKVLITVLD VNDNAPEVVL TSLASSVPEN SPRGTLIALL NVNDQDSEEN GQVICFIQGN LPFKLEKSYG NYYSLVTDIV LDREQVPSYN ITVTATDRGT PPLSTETHIS LNVADTNDNP PVFPQASYSA YIPENNPRGV SLVSVTAHDP DCEENAQITY SLAENTIQGA SLSSYVSINS DTGVLYALSS FDYEQFRDLQ VKVMARDNGH PPLSSNVSLS LFVLDQNDNA PEILYPALPT DGSTGVELAP RSAEPGYLVT KVVAVDRDSG QNAWLSYRLL KASEPGLFSV GLHTGEVRTA RALLDRDALK QSLVVAVQDH GQPPLSATVT LTVAVADSIP QVLADLGSLE SPANSETSDL TLYLVVAVAA VSCVFLAFVI LLLALRLRRW HKSRLLQASG GGLTGAPASH FVGVDGVQAF LQTYSHEVSL TTDSRKSHLI FPQPNYADML VSQESFEKSE PLLLSGDSVF SKDSHGLIEQ APPNTDWRFS QAQRPGTSGS QNGDDTGTWP NNQFDTEMLQ AMILASASEA ADGSSTLGGG AGTMGLSARY GPQFTLQHVP DYRQNVYIPG SNATLTNAAG KRDGKAPAGG NGNKKKSGKK EKK
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Produktmerkmale:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human PCDHGA12 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Three step purification of membrane proteins expressed in baculovirus infected SF9 insect cells:

1. Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot.
2. The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot.
3. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Protein is endotoxin-free.

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Kommentare:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: PCDHGA12 (Protocadherin gamma 12 (PCDHGA12))

Andere Bezeichnung: PCDHGA12 (PCDHGA12 Produkte)

Synonyme: CDH21 Protein, FIB3 Protein, PCDH-GAMMA-A12 Protein, Pcdh13 Protein, pc2c Protein, protocadherin gamma subfamily A, 12 Protein, PCDHGA12 Protein, Pcdhga12 Protein

Hintergrund: Potential calcium-dependent cell-adhesion protein. May be involved in the establishment and maintenance of specific neuronal connections in the brain.

Molekulargewicht: 98.9 kDa Including tag.

UniProt: O60330