OTUD4 Protein (AA 1-1114) (Strep Tag)

Produktnummer ABIN3094337

Produktdetails

Target: OTUD4 (OTU Domain Containing 4 (OTUD4))

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-1114

Spezies: Human

Quelle: Tobacco (Nicotiana tabacum)

Aufreinigungstag / Konjugat: Dieses OTUD4 Protein ist gelabelt mit Strep Tag.

Applikation: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Sequenz: MEAAVGVPDG GDQGGAGPRE DATPMDAYLR KLGLYRKLVA KDGSCLFRAV AEQVLHSQSR HVEVRMACIH YLRENREKFE AFIEGSFEEY LKRLENPQEW VGQVEISALS LMYRKDFIIY REPNVSPSQV TENNFPEKVL LCFSNGNHYD IVYPIKYKES SAMCQSLLYE LLYEKVFKTD VSKIVMELDT LEVADEDNSE ISDSEDDSCK SKTAAAAADV NGFKPLSGNE QLKNNGNSTS LPLSRKVLKS LNPAVYRNVE YEIWLKSKQA QQKRDYSIAA GLQYEVGDKC QVRLDHNGKF LNADVQGIHS ENGPVLVEEL GKKHTSKNLK APPPESWNTV SGKKMKKPST SGQNFHSDVD YRGPKNPSKP IKAPSALPPR LQHPSGVRQH AFSSHSSGSQ SQKFSSEHKN LSRTPSQIIR KPDRERVEDF DHTSRESNYF GLSPEERREK QAIEESRLLY EIQNRDEQAF PALSSSSVNQ SASQSSNPCV QRKSSHVGDR KGSRRRMDTE ERKDKDSIHG HSQLDKRPEP STLENITDDK YATVSSPSKS KKLECPSPAE QKPAEHVSLS NPAPLLVSPE VHLTPAVPSL PATVPAWPSE PTTFGPTGVP APIPVLSVTQ TLTTGPDSAV SQAHLTPSPV PVSIQAVNQP LMPLPQTLSL YQDPLYPGFP CNEKGDRAIV PPYSLCQTGE DLPKDKNILR FFFNLGVKAY SCPMWAPHSY LYPLHQAYLA ACRMYPKVPV PVYPHNPWFQ EAPAAQNESD CTCTDAHFPM QTEASVNGQM PQPEIGPPTF SSPLVIPPSQ VSESHGQLSY QADLESETPG QLLHADYEES LSGKNMFPQP SFGPNPFLGP VPIAPPFFPH VWYGYPFQGF IENPVMRQNI VLPSDEKGEL DLSLENLDLS KDCGSVSTVD EFPEARGEHV HSLPEASVSS KPDEGRTEQS SQTRKADTAL ASIPPVAEGK AHPPTQILNR ERETVPVELE PKRTIQSLKE KTEKVKDPKT AADVVSPGAN SVDSRVQRPK EESSEDENEV SNILRSGRSK QFYNQTYGSR KYKSDWGYSG RGGYQHVRSE ESWKGQPSRS RDEGYQYHRN VRGRPFRGDR RRSGMGDGHR GQHT
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Produktmerkmale: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin-Niveau: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Kommentare:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: OTUD4 (OTU Domain Containing 4 (OTUD4))

Andere Bezeichnung: OTUD4 (OTUD4 Produkte)

Synonyme: DUBA6 Protein, HIN1 Protein, HSHIN1 Protein, 4930431L18Rik Protein, AI449692 Protein, D8Ertd69e Protein, mKIAA1046 Protein, RGD1305606 Protein, OTU deubiquitinase 4 Protein, OTU domain containing 4 Protein, OTUD4 Protein, Otud4 Protein

Hintergrund: OTU domain-containing protein 4 (EC 3.4.19.12) (HIV-1-induced protein HIN-1),FUNCTION: Deubiquitinase which hydrolyzes the isopeptide bond between the ubiquitin C-terminus and the lysine epsilon-amino group of the target protein (PubMed:23827681, PubMed:25944111, PubMed:29395066). May negatively regulate inflammatory and pathogen recognition signaling in innate immune response. Upon phosphorylation at Ser-202 and Ser-204 residues, via IL-1 receptor and Toll-like receptor signaling pathway, specifically deubiquitinates 'Lys-63'-polyubiquitinated MYD88 adapter protein triggering down-regulation of NF-kappa-B-dependent transcription of inflammatory mediators (PubMed:29395066). Independently of the catalytic activity, acts as a scaffold for alternative deubiquitinases to assemble specific deubiquitinase-substrate complexes. Associates with USP7 and USP9X deubiquitinases to stabilize alkylation repair enzyme ALKBH3, thereby promoting the repair of alkylated DNA lesions (PubMed:25944111). {ECO:0000269|PubMed:23827681, ECO:0000269|PubMed:25944111, ECO:0000269|PubMed:29395066}.

Molekulargewicht: 124.0 kDa

UniProt: Q01804