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CCRN4L Protein (AA 1-431) (His tag)

Crystallography grade CCRN4L Spezies: Human Wirt: Insektenzellen Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Produktnummer ABIN3094238
  • Target Alle CCRN4L Proteine anzeigen
    CCRN4L (CCR4 Carbon Catabolite Repression 4-Like (CCRN4L))
    Protein-Typ
    Recombinant
    Proteineigenschaft
    AA 1-431
    Spezies
    • 3
    • 1
    • 1
    • 1
    Human
    Quelle
    • 2
    • 2
    • 1
    • 1
    Insektenzellen
    Aufreinigungstag / Konjugat
    Dieses CCRN4L Protein ist gelabelt mit His tag.
    Applikation
    Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)
    Sequenz
    MFHSPRRLCS ALLQRDAPGL RRLPAPGLRR PLSPPAAVPR PASPRLLAAA SAASGAARSC SRTVCSMGTG TSRLYSALAK TLNSSAASQH PEYLVSPDPE HLEPIDPKEL LEECRAVLHT RPPRFQRDFV DLRTDCPSTH PPIRVMQWNI LAQALGEGKD NFVQCPVEAL KWEERKCLIL EEILAYQPDI LCLQEVDHYF DTFQPLLSRL GYQGTFFPKP WSPCLDVEHN NGPDGCALFF LQNRFKLVNS ANIRLTAMTL KTNQVAIAQT LECKESGRQF CIAVTHLKAR TGWERFRSAQ GCDLLQNLQN ITQGAKIPLI VCGDFNAEPT EEVYKHFASS SLNLNSAYKL LSADGQSEPP YTTWKIRTSG ECRHTLDYIW YSKHALNVRS ALDLLTEEQI GPNRLPSFNY PSDHLSLVCD FSFTEESDGL S
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Produktmerkmale
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Human NOCT Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Aufreinigung
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Reinheit
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterilität
    0.22 μm filtered
    Endotoxin-Niveau
    Protein is endotoxin free.
    Güteklasse
    Crystallography grade
    Top Product
    Discover our top product CCRN4L Protein
  • Applikationshinweise
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Kommentare

    In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handhabung
    Avoid repeated freeze-thaw cycles.
    Lagerung
    -80 °C
    Informationen zur Lagerung
    Store at -80°C.
    Haltbarkeit
    Unlimited (if stored properly)
  • Target
    CCRN4L (CCR4 Carbon Catabolite Repression 4-Like (CCRN4L))
    Andere Bezeichnung
    NOCT (CCRN4L Produkte)
    Synonyme
    CCR4L Protein, NOC Protein, AU043840 Protein, Ccr4 Protein, nocturnin Protein, Ccrn4lb Protein, MNOC Protein, ccr4l Protein, noc Protein, MGC85016 Protein, ccrn4l Protein, CCRN4L Protein, nocturnin Protein, nocturnin S homeolog Protein, nocturnin L homeolog Protein, putative nocturnin Protein, NOCT Protein, Noct Protein, noct Protein, noct.S Protein, noct.L Protein, LOC5570471 Protein, CpipJ_CPIJ011434 Protein, Smp_105040 Protein
    Hintergrund
    Circadian deadenylase which plays an important role in post-transcriptional regulation of metabolic genes under circadian control. Degrades poly(A) tails of specific target mRNAs leading to their degradation and suppression of translation. Exerts a rhythmic post-transcriptional control of genes necessary for metabolic functions including nutrient absorption, glucose/insulin sensitivity, lipid metabolism, adipogenesis, inflammation and osteogenesis. Plays an important role in favoring adipogenesis over osteoblastogenesis and acts as a key regulator of the adipogenesis/osteogenesis balance. Promotes adipogenesis by activating PPARG transcriptional activity in a deadenylase-independent manner by facilitating its nuclear translocation. Regulates circadian expression of NOS2 in the liver and negatively regulates the circadian expression of IGF1 in the bone. Critical for proper development of early embryos (By similarity). {ECO:0000250}.
    Molekulargewicht
    49.1 kDa Including tag.
    UniProt
    Q9UK39
    Pathways
    Ribonucleoprotein Complex Subunit Organization
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