Nucleolar Protein 6 Protein (NOL6) (AA 1-1146) (Strep Tag)

Produktnummer ABIN3094180

Produktdetails

Target: Nucleolar Protein 6 (NOL6)

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-1146

Spezies: Human

Quelle: Tobacco (Nicotiana tabacum)

Aufreinigungstag / Konjugat: Dieses Nucleolar Protein 6 Protein ist gelabelt mit Strep Tag.

Applikation: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Sequenz: MGPAPAGEQL RGATGEPEVM EPALEGTGKE GKKASSRKRT LAEPPAKGLL QPVKLSRAEL YKEPTNEELN RLRETEILFH SSLLRLQVEE LLKEVRLSEK KKDRIDAFLR EVNQRVVRVP SVPETELTDQ AWLPAGVRVP LHQVPYAVKG CFRFLPPAQV TVVGSYLLGT CIRPDINVDV ALTMPREILQ DKDGLNQRYF RKRALYLAHL AHHLAQDPLF GSVCFSYTNG CHLKPSLLLR PRGKDERLVT VRLHPCPPPD FFRPCRLLPT KNNVRSAWYR GQSPAGDGSP EPPTPRYNTW VLQDTVLESH LQLLSTILSS AQGLKDGVAL LKVWLRQREL DKGQGGFTGF LVSMLVVFLV STRKIHTTMS GYQVLRSVLQ FLATTDLTVN GISLCLSSDP SLPALADFHQ AFSVVFLDSS GHLNLCADVT ASTYHQVQHE ARLSMMLLDS RADDGFHLLL MTPKPMIRAF DHVLHLRPLS RLQAACHRLK LWPELQDNGG DYVSAALGPL TTLLEQGLGA RLNLLAHSRP PVPEWDISQD PPKHKDSGTL TLGLLLRPEG LTSVLELGPE ADQPEAAKFR QFWGSRSELR RFQDGAIREA VVWEAASMSQ KRLIPHQVVT HLLALHADIP ETCVHYVGGP LDALIQGLKE TSSTGEEALV AAVRCYDDLS RLLWGLEGLP LTVSAVQGAH PVLRYTEVFP PTPVRPAFSF YETLRERSSL LPRLDKPCPA YVEPMTVVCH LEGSGQWPQD AEAVQRVRAA FQLRLAELLT QQHGLQCRAT ATHTDVLKDG FVFRIRVAYQ REPQILKEVQ SPEGMISLRD TAASLRLERD TRQLPLLTSA LHGLQQQHPA FSGVARLAKR WVRAQLLGEG FADESLDLVA AALFLHPEPF TPPSSPQVGF LRFLFLVSTF DWKNNPLFVN LNNELTVEEQ VEIRSGFLAA RAQLPVMVIV TPQDRKNSVW TQDGPSAQIL QQLVVLAAEA LPMLEKQLMD PRGPGDIRTV FRPPLDIYDV LIRLSPRHIP RHRQAVDSPA ASFCRGLLSQ PGPSSLMPVL GYDPPQLYLT QLREAFGDLA LFFYDQHGGE VIGVLWKPTS FQPQPFKASS TKGRMVMSRG GELVMVPNVE AILEDFAVLG EGLVQTVEAR SERWTV
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Produktmerkmale: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin-Niveau: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Kommentare:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: Nucleolar Protein 6 (NOL6)

Andere Bezeichnung: NOL6 (NOL6 Produkte)

Synonyme: NRAP Protein, UTP22 Protein, bA311H10.1 Protein, nrap Protein, utp22 Protein, AA410091 Protein, Nrap Protein, nucleolar protein 6 Protein, nucleolar protein 6 L homeolog Protein, nucleolar protein family 6 (RNA-associated) Protein, NOL6 Protein, Nol6 Protein, nol6.L Protein

Hintergrund: Nucleolar protein 6 (Nucleolar RNA-associated protein) (Nrap),FUNCTION: Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome. {ECO:0000269|PubMed:11895476, ECO:0000269|PubMed:34516797}.

Molekulargewicht: 127.6 kDa

UniProt: Q9H6R4