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NEFM Protein (AA 2-916) (His tag)

Crystallography grade NEFM Spezies: Human Wirt: Insektenzellen Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Produktnummer ABIN3094129
  • Target Alle NEFM Proteine anzeigen
    NEFM (Neurofilament Triplet M Protein (NEFM))
    Protein-Typ
    Recombinant
    Proteineigenschaft
    AA 2-916
    Spezies
    • 4
    • 2
    • 1
    • 1
    Human
    Quelle
    • 3
    • 1
    • 1
    • 1
    • 1
    Insektenzellen
    Aufreinigungstag / Konjugat
    Dieses NEFM Protein ist gelabelt mit His tag.
    Applikation
    SDS-PAGE (SDS), Western Blotting (WB), ELISA, Crystallization (Crys)
    Sequenz
    SYTLDSLGNP SAYRRVTETR SSFSRVSGSP SSGFRSQSWS RGSPSTVSSS YKRSMLAPRL AYSSAMLSSA ESSLDFSQSS SLLNGGSGPG GDYKLSRSNE KEQLQGLNDR FAGYIEKVHY LEQQNKEIEA EIQALRQKQA SHAQLGDAYD QEIRELRATL EMVNHEKAQV QLDSDHLEED IHRLKERFEE EARLRDDTEA AIRALRKDIE EASLVKVELD KKVQSLQDEV AFLRSNHEEE VADLLAQIQA SHITVERKDY LKTDISTALK EIRSQLESHS DQNMHQAEEW FKCRYAKLTE AAEQNKEAIR SAKEEIAEYR RQLQSKSIEL ESVRGTKESL ERQLSDIEER HNHDLSSYQD TIQQLENELR GTKWEMARHL REYQDLLNVK MALDIEIAAY RKLLEGEETR FSTFAGSITG PLYTHRPPIT ISSKIQKPKV EAPKLKVQHK FVEEIIEETK VEDEKSEMEE ALTAITEELA VSMKEEKKEA AEEKEEEPEA EEEEVAAKKS PVKATAPEVK EEEGEKEEEE GQEEEEEEDE GAKSDQAEEG GSEKEGSSEK EEGEQEEGET EAEAEGEEAE AKEEKKVEEK SEEVATKEEL VADAKVEKPE KAKSPVPKSP VEEKGKSPVP KSPVEEKGKS PVPKSPVEEK GKSPVPKSPV EEKGKSPVSK SPVEEKAKSP VPKSPVEEAK SKAEVGKGEQ KEEEEKEVKE APKEEKVEKK EEKPKDVPEK KKAESPVKEE AVAEVVTITK SVKVHLEKET KEEGKPLQQE KEKEKAGGEG GSEEEGSDKG AKGSRKEDIA VNGEVEGKEE VEQETKEKGS GREEEKGVVT NGLDLSPADE KKGGDKSEEK VVVTKTVEKI TSEGGDGATK YITKSVTVTQ KVEEHEETFE EKLVSTKKVE KVTSHAIVKE VTQSD
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Produktmerkmale
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Human NEFM Protein (raised in E. Coli) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Aufreinigung
    Two step purification of proteins expressed in bacterial culture:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Reinheit
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterilität
    0.22 μm filtered
    Endotoxin-Niveau
    Protein is endotoxin free.
    Güteklasse
    Crystallography grade
  • Applikationshinweise
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Kommentare

    In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handhabung
    Avoid repeated freeze-thaw cycles.
    Lagerung
    -80 °C
    Informationen zur Lagerung
    Store at -80°C.
    Haltbarkeit
    Unlimited (if stored properly)
  • Target
    NEFM (Neurofilament Triplet M Protein (NEFM))
    Andere Bezeichnung
    NEFM (NEFM Produkte)
    Synonyme
    NEF3 Protein, NF-M Protein, NFM Protein, Nef3 Protein, Nfm Protein, NF160 Protein, NF165 Protein, nef-m Protein, nef3 Protein, nefm Protein, nf-m Protein, zgc:112359 Protein, nefm-a Protein, nefm-b Protein, neurofilament medium Protein, neurofilament triplet M protein Protein, neurofilament, medium polypeptide Protein, neurofilament medium polypeptide Protein, neurofilament medium L homeolog Protein, neurofilament, medium polypeptide a Protein, neurofilament medium S homeolog Protein, NEFM Protein, Nefm Protein, TVAG_582010 Protein, TVAG_379630 Protein, nefm.L Protein, nefma Protein, nefm.S Protein
    Hintergrund
    Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber.
    Molekulargewicht
    103.3 kDa Including tag.
    UniProt
    P07197
    Pathways
    Brown Fat Cell Differentiation
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