MYT1L Protein (AA 1-1186) (Strep Tag)

Produktnummer ABIN3094002

Produktdetails

Target: MYT1L (Myelin Transcription Factor 1-Like (MYT1L))

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-1186

Spezies: Human

Quelle: Tobacco (Nicotiana tabacum)

Aufreinigungstag / Konjugat: Dieses MYT1L Protein ist gelabelt mit Strep Tag.

Applikation: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Sequenz: MEVDTEEKRH RTRSKGVRVP VEPAIQELFS CPTPGCDGSG HVSGKYARHR SVYGCPLAKK RKTQDKQPQE PAPKRKPFAV KADSSSVDEC DDSDGTEDMD EKEEDEGEEY SEDNDEPGDE DEEDEEGDRE EEEEIEEEDE DDDEDGEDVE DEEEEEEEEE EEEEEEENED HQMNCHNTRI MQDTEKDDNN NDEYDNYDEL VAKSLLNLGK IAEDAAYRAR TESEMNSNTS NSLEDDSDKN ENLGRKSELS LDLDSDVVRE TVDSLKLLAQ GHGVVLSENM NDRNYADSMS QQDSRNMNYV MLGKPMNNGL MEKMVEESDE EVCLSSLECL RNQCFDLARK LSETNPQERN PQQNMNIRQH VRPEEDFPGR TPDRNYSDML NLMRLEEQLS PRSRVFASCA KEDGCHERDD DTTSVNSDRS EEVFDMTKGN LTLLEKAIAL ETERAKAMRE KMAMEAGRRD NMRSYEDQSP RQLPGEDRKP KSSDSHVKKP YYGKDPSRTE KKESKCPTPG CDGTGHVTGL YPHHRSLSGC PHKDRVPPEI LAMHESVLKC PTPGCTGRGH VNSNRNSHRS LSGCPIAAAE KLAKAQEKHQ SCDVSKSSQA SDRVLRPMCF VKQLEIPQYG YRNNVPTTTP RSNLAKELEK YSKTSFEYNS YDNHTYGKRA IAPKVQTRDI SPKGYDDAKR YCKDPSPSSS STSSYAPSSS SNLSCGGGSS ASSTCSKSSF DYTHDMEAAH MAATAILNLS TRCREMPQNL STKPQDLCAT RNPDMEVDEN GTLDLSMNKQ RPRDSCCPIL TPLEPMSPQQ QAVMNNRCFQ LGEGDCWDLP VDYTKMKPRR IDEDESKDIT PEDLDPFQEA LEERRYPGEV TIPSPKPKYP QCKESKKDLI TLSGCPLADK SIRSMLATSS QELKCPTPGC DGSGHITGNY ASHRSLSGCP RAKKSGIRIA QSKEDKEDQE PIRCPVPGCD GQGHITGKYA SHRSASGCPL AAKRQKDGYL NGSQFSWKSV KTEGMSCPTP GCDGSGHVSG SFLTHRSLSG CPRATSAMKK AKLSGEQMLT IKQRASNGIE NDEEIKQLDE EIKELNESNS QMEADMIKLR TQITTMESNL KTIEEENKVI EQQNESLLHE LANLSQSLIH SLANIQLPHM DPINEQNFDA YVTTLTEMYT NQDRYQSPEN KALLENIKQA VRGIQV
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Produktmerkmale: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin-Niveau: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Kommentare:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: MYT1L (Myelin Transcription Factor 1-Like (MYT1L))

Andere Bezeichnung: MYT1L (MYT1L Produkte)

Synonyme: 2900046C06Rik Protein, 2900093J19Rik Protein, C630034G21Rik Protein, Nztf1 Protein, Pmng1 Protein, Png-1 Protein, mKIAA1106 Protein, Nzf-1 Protein, Nzf1 Protein, NZF1 Protein, ZC2HC4B Protein, si:dkey-260n20.1 Protein, myelin transcription factor 1-like Protein, myelin transcription factor 1 like Protein, myelin transcription factor 1-like, a Protein, Myt1l Protein, MYT1L Protein, myt1la Protein

Hintergrund: Myelin transcription factor 1-like protein (MyT1-L) (MyT1L),FUNCTION: Transcription factor that plays a key role in neuronal differentiation by specifically repressing expression of non-neuronal genes during neuron differentiation. In contrast to other transcription repressors that inhibit specific lineages, mediates repression of multiple differentiation programs. Also represses expression of negative regulators of neurogenesis, such as members of the Notch signaling pathway, including HES1. The combination of three transcription factors, ASCL1, POU3F2/BRN2 and MYT1L, is sufficient to reprogram fibroblasts and other somatic cells into induced neuronal (iN) cells in vitro. Directly binds the 5'-AAGTT-3' core motif present on the promoter of target genes and represses transcription by recruiting a multiprotein complex containing SIN3B. The 5'-AAGTT-3' core motif is absent from the promoter of neural genes. {ECO:0000250|UniProtKB:P97500}.

Molekulargewicht: 133.0 kDa

UniProt: Q9UL68