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Mre11 Protein (AA 2-708) (His tag)

Crystallography grade MRE11A Spezies: Human Wirt: Insektenzellen Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Produktnummer ABIN3093919
  • Target Alle Mre11 (MRE11A) Proteine anzeigen
    Mre11 (MRE11A) (MRE11 Meiotic Recombination 11 Homolog A (S. Cerevisiae) (MRE11A))
    Protein-Typ
    Recombinant
    Proteineigenschaft
    AA 2-708
    Spezies
    • 3
    • 1
    • 1
    Human
    Quelle
    • 1
    • 1
    • 1
    • 1
    • 1
    Insektenzellen
    Aufreinigungstag / Konjugat
    Dieses Mre11 Protein ist gelabelt mit His tag.
    Applikation
    Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)
    Sequenz
    STADALDDEN TFKILVATDI HLGFMEKDAV RGNDTFVTLD EILRLAQENE VDFILLGGDL FHENKPSRKT LHTCLELLRK YCMGDRPVQF EILSDQSVNF GFSKFPWVNY QDGNLNISIP VFSIHGNHDD PTGADALCAL DILSCAGFVN HFGRSMSVEK IDISPVLLQK GSTKIALYGL GSIPDERLYR MFVNKKVTML RPKEDENSWF NLFVIHQNRS KHGSTNFIPE QFLDDFIDLV IWGHEHECKI APTKNEQQLF YISQPGSSVV TSLSPGEAVK KHVGLLRIKG RKMNMHKIPL HTVRQFFMED IVLANHPDIF NPDNPKVTQA IQSFCLEKIE EMLENAERER LGNSHQPEKP LVRLRVDYSG GFEPFSVLRF SQKFVDRVAN PKDIIHFFRH REQKEKTGEE INFGKLITKP SEGTTLRVED LVKQYFQTAE KNVQLSLLTE RGMGEAVQEF VDKEEKDAIE ELVKYQLEKT QRFLKERHID ALEDKIDEEV RRFRETRQKN TNEEDDEVRE AMTRARALRS QSEESASAFS ADDLMSIDLA EQMANDSDDS ISAATNKGRG RGRGRRGGRG QNSASRGGSQ RGRADTGLET STRSRNSKTA VSASRNMSII DAFKSTRQQP SRNVTTKNYS EVIEVDESDV EEDIFPTTSK TDQRWSSTSS SKIMSQSQVS KGVDFESSED DDDDPFMNTS SLRRNRR
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Produktmerkmale
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Human MRE11A Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Aufreinigung
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Reinheit
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterilität
    0.22 μm filtered
    Endotoxin-Niveau
    Protein is endotoxin free.
    Güteklasse
    Crystallography grade
    Top Product
    Discover our top product MRE11A Protein
  • Applikationshinweise
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Kommentare

    In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handhabung
    Avoid repeated freeze-thaw cycles.
    Lagerung
    -80 °C
    Informationen zur Lagerung
    Store at -80°C.
    Haltbarkeit
    Unlimited (if stored properly)
  • Target
    Mre11 (MRE11A) (MRE11 Meiotic Recombination 11 Homolog A (S. Cerevisiae) (MRE11A))
    Andere Bezeichnung
    MRE11A (MRE11A Produkte)
    Synonyme
    wu:faa63d12 Protein, zgc:64018 Protein, Mre11 Protein, Mre11b Protein, ATLD Protein, HNGS1 Protein, MRE11 Protein, MRE11B Protein, MRE11 homolog A, double strand break repair nuclease Protein, MRE11 homolog, double strand break repair nuclease Protein, MRE11A homolog A, double strand break repair nuclease Protein, mre11a Protein, MRE11 Protein, Mre11a Protein
    Hintergrund
    Component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11A. RAD50 may be required to bind DNA ends and hold them in close proximity. This could facilitate searches for short or long regions of sequence homology in the recombining DNA templates, and may also stimulate the activity of DNA ligases and/or restrict the nuclease activity of MRE11A to prevent nucleolytic degradation past a given point (PubMed:9651580, PubMed:9590181, PubMed:9705271, PubMed:11741547). The complex may also be required for DNA damage signaling via activation of the ATM kinase (PubMed:15064416). In telomeres the MRN complex may modulate t-loop formation (PubMed:10888888). {ECO:0000269|PubMed:10888888, ECO:0000269|PubMed:11741547, ECO:0000269|PubMed:15064416, ECO:0000269|PubMed:9590181, ECO:0000269|PubMed:9705271}.
    Molekulargewicht
    81.4 kDa Including tag.
    UniProt
    P49959
    Pathways
    DNA Reparatur
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