LIMCH1 Protein (AA 1-1083) (Strep Tag)

Produktnummer ABIN3093581

Produktdetails

Target: LIMCH1 (LIM and Calponin Homology Domains 1 (LIMCH1))

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-1083

Spezies: Human

Quelle: Tobacco (Nicotiana tabacum)

Aufreinigungstag / Konjugat: Dieses LIMCH1 Protein ist gelabelt mit Strep Tag.

Applikation: Western Blotting (WB), SDS-PAGE (SDS), ELISA

Sequenz: MACPALGLEA LQPLQPEPPP EPAFSEAQKW IEQVTGRSFG DKDFRTGLEN GILLCELLNA IKPGLVKKIN RLPTPIAGLD NIILFLRGCK ELGLKESQLF DPSDLQDTSN RVTVKSLDYS RKLKNVLVTI YWLGKAANSC TSYSGTTLNL KEFEGLLAQM RKDTDDIESP KRSIRDSGYI DCWDSERSDS LSPPRHGRDD SFDSLDSFGS RSRQTPSPDV VLRGSSDGRG SDSESDLPHR KLPDVKKDDM SARRTSHGEP KSAVPFNQYL PNKSNQTAYV PAPLRKKKAE REEYRKSWST ATSPLGGERP FRYGPRTPVS DDAESTSMFD MRCEEEAAVQ PHSRARQEQL QLINNQLREE DDKWQDDLAR WKSRRRSVSQ DLIKKEEERK KMEKLLAGED GTSERRKSIK TYREIVQEKE RRERELHEAY KNARSQEEAE GILQQYIERF TISEAVLERL EMPKILERSH STEPNLSSFL NDPNPMKYLR QQSLPPPKFT ATVETTIARA SVLDTSMSAG SGSPSKTVTP KAVPMLTPKP YSQPKNSQDV LKTFKVDGKV SVNGETVHRE EEKERECPTV APAHSLTKSQ MFEGVARVHG SPLELKQDNG SIEINIKKPN SVPQELAATT EKTEPNSQED KNDGGKSRKG NIELASSEPQ HFTTTVTRCS PTVAFVEFPS SPQLKNDVSE EKDQKKPENE MSGKVELVLS QKVVKPKSPE PEATLTFPFL DKMPEANQLH LPNLNSQVDS PSSEKSPVMT PQFKFWAWDP EEERRRQEKW QQEQERLLQE RYQKEQDKLK EEWEKAQKEV EEEERRYYEE ERKIIEDTVV PFTVSSSSAD QLSTSSSMTE GSGTMNKIDL GNCQDEKQDR RWKKSFQGDD SDLLLKTRES DRLEEKGSLT EGALAHSGNP VSKGVHEDHQ LDTEAGAPHC GTNPQLAQDP SQNQQTSNPT HSSEDVKPKT LPLDKSINHQ IESPSERRKK SPREHFQAGP FSPCSPTPPG QSPNRSISGK KLCSSCGLPL GKGAAMIIET LNLYFHIQCF RCGICKGQLG DAVSGTDVRI RNGLLNCNDC YMRSRSAGQP TTL
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Produktmerkmale: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin-Niveau: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Kommentare:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: LIMCH1 (LIM and Calponin Homology Domains 1 (LIMCH1))

Andere Bezeichnung: LIMCH1 (LIMCH1 Produkte)

Synonyme: 3732412D22Rik Protein, mKIAA1102 Protein, LIMCH1A Protein, LMO7B Protein, RGD1305269 Protein, fk03g12 Protein, limch1 Protein, wu:fc38d05 Protein, wu:fk03g12 Protein, zgc:175186 Protein, LIM and calponin homology domains 1 Protein, LIM and calponin homology domains 1 L homeolog Protein, LIM and calponin homology domains 1b Protein, Limch1 Protein, LIMCH1 Protein, limch1.L Protein, limch1b Protein

Hintergrund: LIM and calponin homology domains-containing protein 1,FUNCTION: Actin stress fibers-associated protein that activates non-muscle myosin IIa. Activates the non-muscle myosin IIa complex by promoting the phosphorylation of its regulatory subunit MRLC/MYL9. Through the activation of non-muscle myosin IIa, positively regulates actin stress fibers assembly and stabilizes focal adhesions. It therefore negatively regulates cell spreading and cell migration. {ECO:0000269|PubMed:28228547}.

Molekulargewicht: 121.9 kDa

UniProt: Q9UPQ0