LNPEP Protein (AA 132-1025) (His tag)

Produktnummer ABIN3093476

Produktdetails

Target: LNPEP (Leucyl/cystinyl Aminopeptidase (LNPEP))

Protein-Typ: Recombinant

Proteineigenschaft: AA 132-1025

Spezies: Human

Quelle: Insektenzellen

Aufreinigungstag / Konjugat: Dieses LNPEP Protein ist gelabelt mit His tag.

Applikation: Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)

Sequenz: MKTIIALSYI FCLVFA PRCTFTKEGC HKKNQSIGLI QPFATNGKLF PWAQIRLPTA VVPLRYELSL HPNLTSMTFR GSVTISVQAL QVTWNIILHS TGHNISRVTF MSAVSSQEKQ AEILEYAYHG QIAIVAPEAL LAGHNYTLKI EYSANISSSY YGFYGFSYTD ESNEKKYFAA TQFEPLAARS AFPCFDEPAF KATFIIKIIR DEQYTALSNM PKKSSVVLDD GLVQDEFSES VKMSTYLVAF IVGEMKNLSQ DVNGTLVSIY AVPEKIGQVH YALETTVKLL EFFQNYFEIQ YPLKKLDLVA IPDFEAGAME NWGLLTFREE TLLYDSNTSS MADRKLVTKI IAHELAHQWF GNLVTMKWWN DLWLNEGFAT FMEYFSLEKI FKELSSYEDF LDARFKTMKK DSLNSSHPIS SSVQSSEQIE EMFDSLSYFK GSSLLLMLKT YLSEDVFQHA VVLYLHNHSY ASIQSDDLWD SFNEVTNQTL DVKRMMKTWT LQKGFPLVTV QKKGKELFIQ QERFFLNMKP EIQPSDTSYL WHIPLSYVTE GRNYSKYQSV SLLDKKSGVI NLTEEVLWVK VNINMNGYYI VHYADDDWEA LIHQLKINPY VLSDKDRANL INNIFELAGL GKVPLKRAFD LINYLGNENH TAPITEALFQ TDLIYNLLEK LGYMDLASRL VTRVFKLLQN QIQQQTWTDE GTPSMRELRS ALLEFACTHN LGNCSTTAMK LFDDWMASNG TQSLPTDVMT TVFKVGAKTD KGWSFLLGKY ISIGSEAEKN KILEALASSE DVRKLYWLMK SSLNGDNFRT QKLSFIIRTV GRHFPGHLLA WDFVKENWNK LVQKFPLGSY TIQNIVAGST YLFSTKTHLS EVQAFFENQS EATFRLRCVQ EALEVIQLNI QWMEKNLKSL TWWLHHHHHH

Produktmerkmale:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human LNPEP Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: The protein is purified from the cleared cell lysate using His-tag capture materials. Eluate fractions are analyzed by SDS-PAGE. Protein containing fractions are subjected to a second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Protein is endotoxin free.

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Kommentare:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 30 mM Hepes, pH 7.4, 2,5 mM EDTA, 100 mM NaCl

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: LNPEP (Leucyl/cystinyl Aminopeptidase (LNPEP))

Andere Bezeichnung: LNPEP (LNPEP Produkte)

Synonyme: fb73h12 Protein, wu:fb73h12 Protein, zgc:66103 Protein, 2010309L07Rik Protein, 4732490P18Rik Protein, CAP Protein, IRAP Protein, PLAP Protein, gp160 Protein, vp165 Protein, P-LAP Protein, Vp165 Protein, LNPEP Protein, leucyl and cystinyl aminopeptidase Protein, leucyl/cystinyl aminopeptidase L homeolog Protein, leucyl/cystinyl aminopeptidase Protein, LNPEP Protein, lnpep.L Protein, lnpep Protein, Lnpep Protein

Hintergrund: Release of an N-terminal amino acid, cleaves before cysteine, leucine as well as other amino acids. Degrades peptide hormones such as oxytocin, vasopressin and angiotensin III, and plays a role in maintaining homeostasis during pregnancy. May be involved in the inactivation of neuronal peptides in the brain. Cleaves Met-enkephalin and dynorphin. Binds angiotensin IV and may be the angiotensin IV receptor in the brain. {ECO:0000269|PubMed:11389728, ECO:0000269|PubMed:11707427, ECO:0000269|PubMed:1731608}.

Molekulargewicht: 103.6 kDa Including tag.

UniProt: Q9UIQ6

Pathways: Regulation of long-term Neuronal Synaptic Plasticity