HSP90AA1 Protein (AA 2-732) (His tag)

Produktnummer ABIN3093026

Produktdetails

Target: HSP90AA1 (Heat Shock Protein 90kDa alpha (Cytosolic), Class A Member 1 (HSP90AA1))

Protein-Typ: Recombinant

Proteineigenschaft: AA 2-732

Spezies: Human

Quelle: Insektenzellen

Aufreinigungstag / Konjugat: Dieses HSP90AA1 Protein ist gelabelt mit His tag.

Applikation: Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)

Sequenz: PEETQTQDQP MEEEEVETFA FQAEIAQLMS LIINTFYSNK EIFLRELISN SSDALDKIRY ESLTDPSKLD SGKELHINLI PNKQDRTLTI VDTGIGMTKA DLINNLGTIA KSGTKAFMEA LQAGADISMI GQFGVGFYSA YLVAEKVTVI TKHNDDEQYA WESSAGGSFT VRTDTGEPMG RGTKVILHLK EDQTEYLEER RIKEIVKKHS QFIGYPITLF VEKERDKEVS DDEAEEKEDK EEEKEKEEKE SEDKPEIEDV GSDEEEEKKD GDKKKKKKIK EKYIDQEELN KTKPIWTRNP DDITNEEYGE FYKSLTNDWE DHLAVKHFSV EGQLEFRALL FVPRRAPFDL FENRKKKNNI KLYVRRVFIM DNCEELIPEY LNFIRGVVDS EDLPLNISRE MLQQSKILKV IRKNLVKKCL ELFTELAEDK ENYKKFYEQF SKNIKLGIHE DSQNRKKLSE LLRYYTSASG DEMVSLKDYC TRMKENQKHI YYITGETKDQ VANSAFVERL RKHGLEVIYM IEPIDEYCVQ QLKEFEGKTL VSVTKEGLEL PEDEEEKKKQ EEKKTKFENL CKIMKDILEK KVEKVVVSNR LVTSPCCIVT STYGWTANME RIMKAQALRD NSTMGYMAAK KHLEINPDHS IIETLRQKAE ADKNDKSVKD LVILLYETAL LSSGFSLEDP QTHANRIYRM IKLGLGIDED DPTADDTSAA VTEEMPPLEG DDDTSRMEEV D
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Produktmerkmale:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human HSP90AA1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Protein is endotoxin free.

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Kommentare:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: HSP90AA1 (Heat Shock Protein 90kDa alpha (Cytosolic), Class A Member 1 (HSP90AA1))

Andere Bezeichnung: HSP90AA1 (HSP90AA1 Produkte)

Synonyme: EL52 Protein, HSP86 Protein, HSP89A Protein, HSP90A Protein, HSP90N Protein, HSPC1 Protein, HSPCA Protein, HSPCAL1 Protein, HSPCAL4 Protein, HSPN Protein, Hsp89 Protein, Hsp90 Protein, LAP2 Protein, Hsp86 Protein, Hspca Protein, htpG Protein, 86kDa Protein, 89kDa Protein, AL024080 Protein, AL024147 Protein, Hsp86-1 Protein, hsp4 Protein, HSP90 Protein, HSP90AA1 Protein, fb17b01 Protein, hsp90 Protein, hsp90a Protein, hsp90a.1 Protein, hsp90alpha Protein, wu:fb17b01 Protein, zgc:86652 Protein, Hsp90alpha Protein, heat shock protein 90 alpha family class A member 1 Protein, heat shock protein 90, alpha (cytosolic), class A member 1 Protein, Heat Shock Protein 90, cytosolic Protein, heat shock protein 90A Protein, molecular chaperone Protein, heat shock protein 90, alpha (cytosolic), class A member 1, tandem duplicate 1 Protein, heat shock protein HSP 90-alpha Protein, heat shock protein 90kDa alpha (cytosolic), class A member 1 Protein, HSP90AA1 Protein, Hsp90aa1 Protein, HSP90A Protein, hsp90A Protein, hsp90aa1.1 Protein, LOC108698781 Protein

Hintergrund: Molecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. Undergoes a functional cycle that is linked to its ATPase activity. This cycle probably induces conformational changes in the client proteins, thereby causing their activation. Interacts dynamically with various co-chaperones that modulate its substrate recognition, ATPase cycle and chaperone function. Binds bacterial lipopolysaccharide (LPS) et mediates LPS-induced inflammatory response, including TNF secretion by monocytes. {ECO:0000269|PubMed:11274138, ECO:0000269|PubMed:11276205, ECO:0000269|PubMed:15577939, ECO:0000269|PubMed:15937123}.

Molekulargewicht: 85.5 kDa Including tag.

UniProt: P07900

Pathways: M Phase, Regulation of Cell Size, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals