Hsc70 Protein (AA 2-646) (His tag)

Produktnummer ABIN3093005

Produktdetails

Target: Hsc70 (HSPA8) (Heat Shock 70kDa Protein 8 (HSPA8))

Protein-Typ: Recombinant

Proteineigenschaft: AA 2-646

Spezies: Human

Quelle: Escherichia coli (E. coli)

Aufreinigungstag / Konjugat: Dieses Hsc70 Protein ist gelabelt mit His tag.

Applikation: Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)

Sequenz: SKGPAVGIDL GTTYSCVGVF QHGKVEIIAN DQGNRTTPSY VAFTDTERLI GDAAKNQVAM NPTNTVFDAK RLIGRRFDDA VVQSDMKHWP FMVVNDAGRP KVQVEYKGET KSFYPEEVSS MVLTKMKEIA EAYLGKTVTN AVVTVPAYFN DSQRQATKDA GTIAGLNVLR IINEPTAAAI AYGLDKKVGA ERNVLIFDLG GGTFDVSILT IEDGIFEVKS TAGDTHLGGE DFDNRMVNHF IAEFKRKHKK DISENKRAVR RLRTACERAK RTLSSSTQAS IEIDSLYEGI DFYTSITRAR FEELNADLFR GTLDPVEKAL RDAKLDKSQI HDIVLVGGST RIPKIQKLLQ DFFNGKELNK SINPDEAVAY GAAVQAAILS GDKSENVQDL LLLDVTPLSL GIETAGGVMT VLIKRNTTIP TKQTQTFTTY SDNQPGVLIQ VYEGERAMTK DNNLLGKFEL TGIPPAPRGV PQIEVTFDID ANGILNVSAV DKSTGKENKI TITNDKGRLS KEDIERMVQE AEKYKAEDEK QRDKVSSKNS LESYAFNMKA TVEDEKLQGK INDEDKQKIL DKCNEIINWL DKNQTAEKEE FEHQQKELEK VCNPIITKLY QSAGGMPGGM PGGFPGGGAP PSGGASSGPT IEEVD
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Produktmerkmale:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human HSPA8 Protein (raised in E. Coli) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in bacterial culture:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Endotoxin has not been removed. Please contact us if you require endotoxin removal.

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Kommentare:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: Hsc70 (HSPA8) (Heat Shock 70kDa Protein 8 (HSPA8))

Andere Bezeichnung: HSPA8 (HSPA8 Produkte)

Synonyme: hsc54 Protein, hsc70 Protein, hsc71 Protein, hsp71 Protein, hsp73 Protein, hspa10 Protein, lap1 Protein, nip71 Protein, HSC54 Protein, HSC70 Protein, HSC71 Protein, HSP71 Protein, HSP73 Protein, HSPA10 Protein, LAP1 Protein, NIP71 Protein, Hsc70 Protein, 2410008N15Rik Protein, Hsc71 Protein, Hsc73 Protein, Hsp73 Protein, Hspa10 Protein, wu:fb01g06 Protein, wu:fi48b06 Protein, heat shock protein family A (Hsp70) member 8 L homeolog Protein, heat shock protein family A (Hsp70) member 8 Protein, heat shock 70kDa protein 8 Protein, heat shock protein 8 Protein, hspa8.L Protein, HSPA8 Protein, Hspa8 Protein, hspa8 Protein

Hintergrund: Acts as a repressor of transcriptional activation. Inhibits the transcriptional coactivator activity of CITED1 on Smad-mediated transcription. Chaperone. Component of the PRP19-CDC5L complex that forms an integral part of the spliceosome and is required for activating pre-mRNA splicing. May have a scaffolding role in the spliceosome assembly as it contacts all other components of the core complex. Binds bacterial lipopolysaccharide (LPS) et mediates LPS-induced inflammatory response, including TNF secretion by monocytes. Participates in the ER-associated degradation (ERAD) quality control pathway in conjunction with J domain-containing co-chaperones and the E3 ligase CHIP. {ECO:0000269|PubMed:10722728, ECO:0000269|PubMed:11276205, ECO:0000269|PubMed:23990462}.

Molekulargewicht: 71.7 kDa Including tag.

UniProt: P11142