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EVC Protein (AA 49-992) (His tag)

Crystallography grade EVC Spezies: Human Wirt: Insektenzellen Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, Crys, SDS
Produktnummer ABIN3092466
  • Target Alle EVC Proteine anzeigen
    EVC (Ellis Van Creveld Syndrome (EVC))
    Protein-Typ
    Recombinant
    Proteineigenschaft
    AA 49-992
    Spezies
    • 4
    • 2
    Human
    Quelle
    • 3
    • 2
    • 1
    Insektenzellen
    Aufreinigungstag / Konjugat
    Dieses EVC Protein ist gelabelt mit His tag.
    Applikation
    ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)
    Sequenz
    RAGRQRTRHQ KDDTQNLLKN LESNAQTPSE TGSPSRRRKR EVQMSKDKEA VDECEPPSNS NITAFALKAK VIYPINQKFR PLADGSSNPS LHENLKQAVL PHQPVEASPS SSLGSLSQGE KDDCSSSSSV HSATSDDRFL SRTFLRVNAF PEVLACESVD VDLCIYSLHL KDLLHLDTAL RQEKHMMFIQ IFKMCLLDLL PKKKSDDELY QKILSKQEKD LEELEKGLQV KLSNTEMSGA GDSEYITLAD VEKKEREYSE QLIDNMEAFW KQMANIQHFL VDQFKCSSSK ARQLMMTLTE RMIAAEGLLC DSQELQALDA LERTMGRAHM AKVIEFLKLQ VQEETRCRLA AISHGLELLA GEGKLSGRQK EELLTQQHKA FWQEAERFSR EFVQRGKDLV TASLAHQVEG TAKLTLAQEE EQRSFLAEAQ PTADPEKFLE AFHEVLERQR LMQCDLEEEE NVRATEAVVA LCQELYFSTV DTFQKFVDAL FLQTLPGMTG LPPEECDYLR QEVQENAAWQ LGKSNRFRRQ QWKLFQELLE QDQQVWMEEC ALSSVLQTHL REDHEGTIRG VLGRLGGLTE ESTRCVLQGH DLLLRSALRR LALRGNALAT LTQMRLSGKK HLLQELREQR ALEQGSSQCL DEHQWQLLRA LEARVLEEAS RLEEEAQQTR LQLQQRLLAE AQEVGQLLQQ HMECAIGQAL LVHARNAATK SRAKDRDDFK RTLMEAAVES VYVTSAGVSR LVQAYYQQIG RIMEDHEERK LQHLKTLQGE RMENYKLRKK QELSNPSSGS RTAGGAHETS QAVHQRMLSQ QKRFLAQFPV HQQMRLHAQQ QQAGVMDLLE AQLETQLQEA EQNFISELAA LARVPLAESK LLPAKRGLLE KPLRTKRKKP LPQERGDLGV PNNEDLASGD QTSGSLSSKR LSQQESEAGD SGNSKKMLKR RSNL
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Produktmerkmale
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Human EVC Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Aufreinigung
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Reinheit
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterilität
    0.22 μm filtered
    Endotoxin-Niveau
    Protein is endotoxin free.
    Güteklasse
    Crystallography grade
    Top Product
    Discover our top product EVC Protein
  • Applikationshinweise
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Kommentare

    In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handhabung
    Avoid repeated freeze-thaw cycles.
    Lagerung
    -80 °C
    Informationen zur Lagerung
    Store at -80°C.
    Haltbarkeit
    Unlimited (if stored properly)
  • Target
    EVC (Ellis Van Creveld Syndrome (EVC))
    Andere Bezeichnung
    EVC (EVC Produkte)
    Synonyme
    EVC Protein, DWF-1 Protein, evcl Protein, dwf-1 Protein, EVC1 Protein, EVCL Protein, EvC ciliary complex subunit 1 Protein, EVC Protein, evc Protein, Evc Protein
    Hintergrund
    Acts as a positive mediator of Hedgehog signaling indispensable for normal endochondral growth and skeletal development. {ECO:0000250}.
    Molekulargewicht
    108.3 kDa Including tag.
    UniProt
    P57679
    Pathways
    Hedgehog Signalweg
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