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ELP3/KAT9 Protein (AA 1-547) (His tag)

Crystallography grade ELP3 Spezies: Human Wirt: Insektenzellen Recombinant >95 % as determined by SDS PAGE and Western Blot. WB, ELISA, Crys, SDS, Func
Produktnummer ABIN3092446
  • Target Alle ELP3/KAT9 (ELP3) Proteine anzeigen
    ELP3/KAT9 (ELP3) (Elongator complex protein 3 (ELP3))
    Protein-Typ
    Recombinant
    Proteineigenschaft
    AA 1-547
    Spezies
    • 2
    • 1
    Human
    Quelle
    • 1
    • 1
    • 1
    • 1
    Insektenzellen
    Aufreinigungstag / Konjugat
    Dieses ELP3/KAT9 Protein ist gelabelt mit His tag.
    Applikation
    Western Blotting (WB), ELISA, Crystallization (Crys), SDS-PAGE (SDS), Functional Studies (Func)
    Sequenz
    MHHHHHHRQKRKGDLSPAELMMLTIGDVIKQLIEAHEQGKDIDLNKVKTKTAAKYGLSAQPRLVDIIAAVPPQYRKVLMPKLKAKPIRTASGIAVVAVMCKPHRCPHISFTGNICVYCPGGPDSDFEYSTQSYTGYEPTSMRAIRARYDPFLQTRHRIEQLKQLGHSVDKVEFIVMGGTFMALPEEYRDYFIRNLHDALSGHTSNNIYEAVKYSERSLTKCIGITIETRPDYCMKRHLSDMLTYGCTRLEIGVQSVYEDVARDTNRGHTVKAVCESFHLAKDSGFKVVAHMMPDLPNVGLERDIEQFTEFFENPAFRPDGLKLYPTLVIRGTGLYELWKSGRYKSYSPSDLVELVARILALVPPWTRVYRVQRDIPMPLVSSGVEHGNLRELALARMKDLGIQCRDVRTREVGIQEIHHKVRPYQVELVRRDYVANGGWETFLSYEDPDQDILIGLLRLRKCSEETFRFELGGGVSIVRELHVYGSVVPVSSRDPTKFQHQGFGMLLMEEAERIAREEHGSGKIAVISGVGTRNYYRKIGYRLQGPYMVKMLK
    Sequence including N-terminal His-tag.
    Produktmerkmale
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Human ELP3 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This made-to-order protein has already been successfully produced. Please let us know if you are interested in purchasing a smaller amount of this protein. We will check our stock and make you a customized quote in case we can provide this protein in a smaller amount.

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    Aufreinigung
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Reinheit
    >95 % as determined by SDS PAGE and Western Blot.
    Sterilität
    0.22 μm filtered
    Endotoxin-Niveau
    Protein is endotoxin free.
    Güteklasse
    Crystallography grade
  • Kommentare

    In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Konzentration
    0.45 mg/mL
    Buffer
    20 mM Hepes, pH 7.4; 300 mM KCl; 10 % Glycerin, 1 % Tween, Protease inhibitors
    Handhabung
    Avoid repeated freeze-thaw cycles.
    Lagerung
    -80 °C
    Informationen zur Lagerung
    Store at -80°C.
    Haltbarkeit
    Unlimited (if stored properly)
  • Target
    ELP3/KAT9 (ELP3) (Elongator complex protein 3 (ELP3))
    Andere Bezeichnung
    ELP3 (ELP3 Produkte)
    Synonyme
    KAT9 Protein, 2610507P14Rik Protein, si:ch211-63o20.8 Protein, zgc:113140 Protein, elongator acetyltransferase complex subunit 3 Protein, elongator acetyltransferase complex subunit 3 L homeolog Protein, ELP3 Protein, Elp3 Protein, elp3.L Protein, elp3 Protein
    Hintergrund
    Catalytic histone acetyltransferase subunit of the RNA polymerase II elongator complex, which is a component of the RNA polymerase II (Pol II) holoenzyme and is involved in transcriptional elongation. Elongator may play a role in chromatin remodeling and is involved in acetylation of histones H3 and probably H4. Involved in acetylation of alpha-tubulin (PubMed:19185337). May also have a methyltransferase activity. Involved in cell migration. Involved in neurogenesis. Regulates the migration and branching of projection neurons in the developing cerebral cortex, through a process depending on alpha-tubulin acetylation (By similarity). {ECO:0000250|UniProtKB:Q9CZX0, ECO:0000269|PubMed:11714725, ECO:0000269|PubMed:11818576, ECO:0000269|PubMed:15902492, ECO:0000269|PubMed:16713582, ECO:0000269|PubMed:19185337}.
    Molekulargewicht
    63.2 kDa Including tag.
    UniProt
    Q9H9T3
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