EDEM1 Protein (AA 26-657) (His tag)

Produktnummer ABIN3092317

Produktdetails

Target: EDEM1 (ER Degradation Enhancer, Mannosidase alpha-Like 1 (EDEM1))

Protein-Typ: Recombinant

Proteineigenschaft: AA 26-657

Spezies: Human

Quelle: Insektenzellen

Aufreinigungstag / Konjugat: Dieses EDEM1 Protein ist gelabelt mit His tag.

Applikation: ELISA, Western Blotting (WB), SDS-PAGE (SDS), Crystallization (Crys)

Sequenz: GLGPSMGFYQ RFPLSFGFQR LRSPDGPASP TSGPVGRPGG VSGPSWLQPP GTGAAQSPRK APRRPGPGMC GPANWGYVLG GRGRGPDEYE KRYSGAFPPQ LRAQMRDLAR GMFVFGYDNY MAHAFPQDEL NPIHCRGRGP DRGDPSNLNI NDVLGNYSLT LVDALDTLAI MGNSSEFQKA VKLVINTVSF DKDSTVQVFE ATIRVLGSLL SAHRIITDSK QPFGDMTIKD YDNELLYMAH DLAVRLLPAF ENTKTGIPYP RVNLKTGVPP DTNNETCTAG AGSLLVEFGI LSRLLGDSTF EWVARRAVKA LWNLRSNDTG LLGNVVNIQT GHWVGKQSGL GAGLDSFYEY LLKSYILFGE KEDLEMFNAA YQSIQNYLRR GREACNEGEG DPPLYVNVNM FSGQLMNTWI DSLQAFFPGL QVLIGDVEDA ICLHAFYYAI WKRYGALPER YNWQLQAPDV LFYPLRPELV ESTYLLYQAT KNPFYLHVGM DILQSLEKYT KVKCGYATLH HVIDKSTEDR MESFFLSETC KYLYLLFDED NPVHKSGTRY MFTTEGHIVS VDEHLRELPW KEFFSEEGGQ DQGGKSVHRP KPHELKVINS SSNCNRVPDE RRYSLPLKSI YMRQIDQMVG LI
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Produktmerkmale:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human EDEM1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Protein is endotoxin free.

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Kommentare:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: EDEM1 (ER Degradation Enhancer, Mannosidase alpha-Like 1 (EDEM1))

Andere Bezeichnung: EDEM1 (EDEM1 Produkte)

Synonyme: zgc:55816 Protein, edem Protein, EDEM1 Protein, EDEM Protein, A130059K23Rik Protein, mKIAA0212 Protein, RGD1563633 Protein, ER degradation enhancer, mannosidase alpha-like 1 Protein, ER degradation enhancing alpha-mannosidase like protein 1 Protein, ER degradation-enhancing alpha-mannosidase-like protein 1 Protein, ER degradation enhancing alpha-mannosidase like protein 1 L homeolog Protein, edem1 Protein, EDEM1 Protein, LOC100637820 Protein, edem1.L Protein, Edem1 Protein

Hintergrund: Extracts misfolded glycoproteins, but not glycoproteins undergoing productive folding, from the calnexin cycle. It is directly involved in endoplasmic reticulum-associated degradation (ERAD) and targets misfolded glycoproteins for degradation in an N-glycan-independent manner, probably by forming a complex with SEL1L. It has low mannosidase activity, catalyzing mannose trimming from Man8GlcNAc2 to Man7GlcNAc2. {ECO:0000269|PubMed:12610306, ECO:0000269|PubMed:19524542, ECO:0000269|PubMed:19934218, ECO:0000269|PubMed:25092655}.

Molekulargewicht: 71.8 kDa Including tag.

UniProt: Q92611

Pathways: ER-Nucleus Signaling