EED Protein (AA 2-441) (His tag)

Produktnummer ABIN3092282

Produktdetails

Target: EED (Embryonic Ectoderm Development (EED))

Protein-Typ: Recombinant

Proteineigenschaft: AA 2-441

Spezies: Human

Quelle: Insektenzellen

Aufreinigungstag / Konjugat: Dieses EED Protein ist gelabelt mit His tag.

Applikation: Western Blotting (WB), ELISA, SDS-PAGE (SDS), Crystallization (Crys)

Sequenz: SEREVSTAPA GTDMPAAKKQ KLSSDENSNP DLSGDENDDA VSIESGTNTE RPDTPTNTPN APGRKSWGKG KWKSKKCKYS FKCVNSLKED HNQPLFGVQF NWHSKEGDPL VFATVGSNRV TLYECHSQGE IRLLQSYVDA DADENFYTCA WTYDSNTSHP LLAVAGSRGI IRIINPITMQ CIKHYVGHGN AINELKFHPR DPNLLLSVSK DHALRLWNIQ TDTLVAIFGG VEGHRDEVLS ADYDLLGEKI MSCGMDHSLK LWRINSKRMM NAIKESYDYN PNKTNRPFIS QKIHFPDFST RDIHRNYVDC VRWLGDLILS KSCENAIVCW KPGKMEDDID KIKPSESNVT ILGRFDYSQC DIWYMRFSMD FWQKMLALGN QVGKLYVWDL EVEDPHKAKC TTLTHHKCGA AIRQTSFSRD SSILIAVCDD ASIWRWDRLR
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Produktmerkmale:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human EED Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Protein is endotoxin free.

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Kommentare:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: EED (Embryonic Ectoderm Development (EED))

Andere Bezeichnung: EED (EED Produkte)

Synonyme: Xeed Protein, heed Protein, wait1 Protein, EED Protein, HEED Protein, WAIT1 Protein, zgc:112509 Protein, Xeed-B Protein, eed Protein, psXEED Protein, ENSMUSG00000039373 Protein, l(7)5Rn Protein, l7Rn5 Protein, lusk Protein, Xeed-A Protein, embryonic ectoderm development Protein, embryonic ectoderm development S homeolog Protein, microRNA 6755 Protein, embryonic ectoderm development L homeolog Protein, eed Protein, EED Protein, eed.S Protein, MIR6755 Protein, Eed Protein, eed.L Protein

Hintergrund: Polycomb group (PcG) protein. Component of the PRC2/EED-EZH2 complex, which methylates 'Lys-9' and 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. Also recognizes 'Lys-26' trimethylated histone H1 with the effect of inhibiting PRC2 complex methyltransferase activity on nucleosomal histone H3 'Lys-27', whereas H3 'Lys-27' recognition has the opposite effect, enabling the propagation of this repressive mark. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1 and CDKN2A. {ECO:0000269|PubMed:10581039, ECO:0000269|PubMed:14532106, ECO:0000269|PubMed:15225548, ECO:0000269|PubMed:15231737, ECO:0000269|PubMed:15385962, ECO:0000269|PubMed:16357870, ECO:0000269|PubMed:18285464, ECO:0000269|PubMed:20974918, ECO:0000269|PubMed:9584199}.

Molekulargewicht: 51.0 kDa Including tag.

UniProt: O75530