DSPP Protein (AA 16-1301) (His tag)

Produktnummer ABIN3092262

Produktdetails

Target: DSPP (Dentin Sialophosphoprotein (DSPP))

Protein-Typ: Recombinant

Proteineigenschaft: AA 16-1301

Spezies: Human

Quelle: Insektenzellen

Aufreinigungstag / Konjugat: Dieses DSPP Protein ist gelabelt mit His tag.

Applikation: SDS-PAGE (SDS), Western Blotting (WB), ELISA, Crystallization (Crys)

Sequenz: IPVPQSKPLE RHVEKSMNLH LLARSNVSVQ DELNASGTIK ESGVLVHEGD RGRQENTQDG HKGEGNGSKW AEVGGKSFST YSTLANEEGN IEGWNGDTGK AETYGHDGIH GKEENITANG IQGQVSIIDN AGATNRSNTN GNTDKNTQNG DVGDAGHNED VAVVQEDGPQ VAGSNNSTDN EDEIIENSCR NEGNTSEITP QINSKRNGTK EAEVTPGTGE DAGLDNSDGS PSGNGADEDE DEGSGDDEDE EAGNGKDSSN NSKGQEGQDH GKEDDHDSSI GQNSDSKEYY DPEGKEDPHN EVDGDKTSKS EENSAGIPED NGSQRIEDTQ KLNHRESKRV ENRITKESET HAVGKSQDKG IEIKGPSSGN RNITKEVGKG NEGKEDKGQH GMILGKGNVK TQGEVVNIEG PGQKSEPGNK VGHSNTGSDS NSDGYDSYDF DDKSMQGDDP NSSDESNGND DANSESDNNS SSRGDASYNS DESKDNGNGS DSKGAEDDDS DSTSDTNNSD SNGNGNNGND DNDKSDSGKG KSDSSDSDSS DSSNSSDSSD SSDSDSSDSN SSSDSDSSDS DSSDSSDSDS SDSSNSSDSS DSSDSSDSSD SSDSSDSKSD SSKSESDSSD SDSKSDSSDS NSSDSSDNSD SSDSSNSSNS SDSSDSSDSS DSSSSSDSSN SSDSSDSSDS SNSSESSDSS DSSDSDSSDS SDSSNSNSSD SDSSNSSDSS DSSNSSDSSD SSDSSNSSDS SDSSDSSNSS DSSDSSDSSD SSDSSNSSDS NDSSNSSDSS DSSNSSDSSN SSDSSDSSDS SDSDSSNSSD SSNSSDSSDS SNSSDSSDSS DSSDGSDSDS SNRSDSSNSS DSSDSSDSSN SSDSSDSSDS NESSNSSDSS DSSNSSDSDS SDSSNSSDSS DSSNSSDSSE SSNSSDNSNS SDSSNSSDSS DSSDSSNSSD SSNSSDSSNS SDSSDSNSSD SSDSSNSSDS SDSSDSSDSS DSSDSSNSSD SSDSSDSSDS SNSSDSSNSS DSSNSSDSSD SSDSSDSSDS SDSSDSSDSS NSSDSSDSSD SSDSSDSSDS SDSSDSSESS DSSDSSNSSD SSDSSDSSDS SDSSDSSDSS DSSDSSNSSD SSDSSDSSDS SDSSNSSDSS DSSESSDSSD SSDSSDSSDS SDSSDSSDSS DSSNSSDSSD SSDSSDSSDS SDSSDSSDSS DSSDSSDSSD SSDSSDSSDS SDSSDSNESS DSSDSSDSSD SSNSSDSSDS SDSSDSTSDS NDESDSQSKS GNGNNNGSDS DSDSEGSDSN HSTSDD
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Produktmerkmale:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human DSPP Protein (raised in E. Coli) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in bacterial culture:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Protein is endotoxin free.

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Kommentare:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: DSPP (Dentin Sialophosphoprotein (DSPP))

Andere Bezeichnung: DSPP (DSPP Produkte)

Synonyme: DFNA39 Protein, DGI1 Protein, DMP3 Protein, DPP Protein, DSP Protein, DTDP2 Protein, Dsp Protein, RDSP2 Protein, Dmp2 Protein, Dmp3 Protein, Dpp Protein, dentin sialophosphoprotein Protein, putative mediator of RNA polymerase II transcription subunit 26 Protein, DSPP Protein, Dspp Protein, LOC551799 Protein, Patl_2314 Protein

Hintergrund: DSP may be an important factor in dentinogenesis. DPP may bind high amount of calcium and facilitate initial mineralization of dentin matrix collagen as well as regulate the size and shape of the crystals.

Molekulargewicht: 130.3 kDa Including tag.

UniProt: Q9NZW4