DENND5A Protein (AA 1-1287) (Strep Tag)

Produktnummer ABIN3092072

Produktdetails

Target: DENND5A (DENN/MADD Domain Containing 5A (DENND5A))

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-1287

Spezies: Human

Quelle: Tobacco (Nicotiana tabacum)

Aufreinigungstag / Konjugat: Dieses DENND5A Protein ist gelabelt mit Strep Tag.

Applikation: ELISA, SDS-PAGE (SDS), Western Blotting (WB)

Sequenz: MSGGGGGGGS APSRFADYFV ICGLDTETGL EPDELSALCQ YIQASKARDG ASPFISSTTE GENFEQTPLR RTFKSKVLAR YPENVEWNPF DQDAVGMLCM PKGLAFKTQA DPREPQFHAF IITREDGSRT FGFALTFYEE VTSKQICSAM QTLYHMHNAE YDVLHAPPAD DRDQSSMEDG EDTPVTKLQR FNSYDISRDT LYVSKCICLI TPMSFMKACR SVLEQLHQAV TSPQPPPLPL ESYIYNVLYE VPLPPPGRSL KFSGVYGPII CQRPSTNELP LFDFPVKEVF ELLGVENVFQ LFTCALLEFQ ILLYSQHYQR LMTVAETITA LMFPFQWQHV YVPILPASLL HFLDAPVPYL MGLHSNGLDD RSKLELPQEA NLCFVDIDNH FIELPEDLPQ FPNKLEFVQE VSEILMAFGI PPEGNLHCSE SASKLKRLRA SELVSDKRNG NIAGSPLHSY ELLKENETIA RLQALVKRTG VSLEKLEVRE DPSSNKDLKV QCDEEELRIY QLNIQIREVF ANRFTQMFAD YEVFVIQPSQ DKESWFTNRE QMQNFDKASF LSDQPEPYLP FLSRFLETQM FASFIDNKIM CHDDDDKDPV LRVFDSRVDK IRLLNVRTPT LRTSMYQKCT TVDEAEKAIE LRLAKIDHTA IHPHLLDMKI GQGKYEPGFF PKLQSDVLST GPASNKWTKR NAPAQWRRKD RQKQHTEHLR LDNDQREKYI QEARTMGSTI RQPKLSNLSP SVIAQTNWKF VEGLLKECRN KTKRMLVEKM GREAVELGHG EVNITGVEEN TLIASLCDLL ERIWSHGLQV KQGKSALWSH LLHYQDNRQR KLTSGSLSTS GILLDSERRK SDASSLMPPL RISLIQDMRH IQNIGEIKTD VGKARAWVRL SMEKKLLSRH LKQLLSDHEL TKKLYKRYAF LRCDDEKEQF LYHLLSFNAV DYFCFTNVFT TILIPYHILI VPSKKLGGSM FTANPWICIS GELGETQIMQ IPRNVLEMTF ECQNLGKLTT VQIGHDNSGL YAKWLVEYVM VRNEITGHTY KFPCGRWLGK GMDDGSLERI LVGELLTSQP EVDERPCRTP PLQQSPSVIR RLVTISPNNK PKLNTGQIQE SIGEAVNGIV KHFHKPEKER GSLTLLLCGE CGLVSALEQA FQHGFKSPRL FKNVFIWDFL EKAQTYYETL EKNEVVPEEN WHTRARNFCR FVTAINNTPR NIGKDGKFQM LVCLGARDHL LHHWIALLAD CPITAHMYED VALIKDHTLV NSLIRVLQTL QEFNITLETS LVKGIDI
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Produktmerkmale: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin-Niveau: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Kommentare:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: DENND5A (DENN/MADD Domain Containing 5A (DENND5A))

Andere Bezeichnung: DENND5A (DENND5A Produkte)

Synonyme: RAB6IP1 Protein, 1500012B19Rik Protein, AJ245569 Protein, AW496491 Protein, ORF37 Protein, Rab6ip1 Protein, mKIAA1091 Protein, DENN domain containing 5A Protein, DENN/MADD domain containing 5A Protein, DENND5A Protein, Dennd5a Protein

Hintergrund: DENN domain-containing protein 5A (Rab6-interacting protein 1) (Rab6IP1),FUNCTION: Guanine nucleotide exchange factor (GEF) which may activate RAB6A and RAB39A and/or RAB39B. Promotes the exchange of GDP to GTP, converting inactive GDP-bound Rab proteins into their active GTP-bound form. Involved in the negative regulation of neurite outgrowth (By similarity). {ECO:0000250|UniProtKB:G3V7Q0, ECO:0000269|PubMed:20937701}.

Molekulargewicht: 147.1 kDa

UniProt: Q6IQ26