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DCC Protein (AA 26-1097) (His tag)

Crystallography grade DCC Spezies: Human Wirt: Insektenzellen Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, ELISA, Crys, SDS
Produktnummer ABIN3092045
  • Target Alle DCC Proteine anzeigen
    DCC (Deleted in Colorectal Carcinoma (DCC))
    Protein-Typ
    Recombinant
    Proteineigenschaft
    AA 26-1097
    Spezies
    • 7
    • 4
    • 1
    Human
    Quelle
    • 5
    • 3
    • 2
    Insektenzellen
    Aufreinigungstag / Konjugat
    Dieses DCC Protein ist gelabelt mit His tag.
    Applikation
    Western Blotting (WB), ELISA, Crystallization (Crys), SDS-PAGE (SDS)
    Sequenz
    HLQVTGFQIK AFTALRFLSE PSDAVTMRGG NVLLDCSAES DRGVPVIKWK KDGIHLALGM DERKQQLSNG SLLIQNILHS RHHKPDEGLY QCEASLGDSG SIISRTAKVA VAGPLRFLSQ TESVTAFMGD TVLLKCEVIG EPMPTIHWQK NQQDLTPIPG DSRVVVLPSG ALQISRLQPG DIGIYRCSAR NPASSRTGNE AEVRILSDPG LHRQLYFLQR PSNVVAIEGK DAVLECCVSG YPPPSFTWLR GEEVIQLRSK KYSLLGGSNL LISNVTDDDS GMYTCVVTYK NENISASAEL TVLVPPWFLN HPSNLYAYES MDIEFECTVS GKPVPTVNWM KNGDVVIPSD YFQIVGGSNL RILGVVKSDE GFYQCVAENE AGNAQTSAQL IVPKPAIPSS SVLPSAPRDV VPVLVSSRFV RLSWRPPAEA KGNIQTFTVF FSREGDNRER ALNTTQPGSL QLTVGNLKPE AMYTFRVVAY NEWGPGESSQ PIKVATQPEL QVPGPVENLQ AVSTSPTSIL ITWEPPAYAN GPVQGYRLFC TEVSTGKEQN IEVDGLSYKL EGLKKFTEYS LRFLAYNRYG PGVSTDDITV VTLSDVPSAP PQNVSLEVVN SRSIKVSWLP PPSGTQNGFI TGYKIRHRKT TRRGEMETLE PNNLWYLFTG LEKGSQYSFQ VSAMTVNGTG PPSNWYTAET PENDLDESQV PDQPSSLHVR PQTNCIIMSW TPPLNPNIVV RGYIIGYGVG SPYAETVRVD SKQRYYSIER LESSSHYVIS LKAFNNAGEG VPLYESATTR SITDPTDPVD YYPLLDDFPT SVPDLSTPML PPVGVQAVAL THDAVRVSWA DNSVPKNQKT SEVRLYTVRW RTSFSASAKY KSEDTTSLSY TATGLKPNTM YEFSVMVTKN RRSSTWSMTA HATTYEAAPT SAPKDLTVIT REGKPRAVIV SWQPPLEANG KITAYILFYT LDKNIPIDDW IMETISGDRL THQIMDLNLD TMYYFRIQAR NSKGVGPLSD PILFRTLKVE HPDKMANDQG RHGDGGYWPV DTNLIDRSTL NEPPIGQMHP PHGSVTPQKN SN
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Produktmerkmale
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Human DCC Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Aufreinigung
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Reinheit
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterilität
    0.22 μm filtered
    Endotoxin-Niveau
    Protein is endotoxin free.
    Güteklasse
    Crystallography grade
    Top Product
    Discover our top product DCC Protein
  • Applikationshinweise
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Kommentare

    In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handhabung
    Avoid repeated freeze-thaw cycles.
    Lagerung
    -80 °C
    Informationen zur Lagerung
    Store at -80°C.
    Haltbarkeit
    Unlimited (if stored properly)
  • Target
    DCC (Deleted in Colorectal Carcinoma (DCC))
    Andere Bezeichnung
    DCC (DCC Produkte)
    Synonyme
    CRC18 Protein, CRCR1 Protein, IGDCC1 Protein, MRMV1 Protein, DCC Protein, zdcc Protein, C030036D22Rik Protein, Igdcc1 Protein, xdcc Protein, XDCCa Protein, dcca-A Protein, Dcc Protein, DCC netrin 1 receptor Protein, deleted in colorectal carcinoma Protein, netrin receptor DCC Protein, DCC Protein, dcc Protein, Dcc Protein, LOC100712666 Protein
    Hintergrund
    Receptor for netrin required for axon guidance. Mediates axon attraction of neuronal growth cones in the developing nervous system upon ligand binding. Its association with UNC5 proteins may trigger signaling for axon repulsion. It also acts as a dependence receptor required for apoptosis induction when not associated with netrin ligand. Implicated as a tumor suppressor gene. {ECO:0000269|PubMed:8187090, ECO:0000269|PubMed:8861902}.
    Molekulargewicht
    119.3 kDa Including tag.
    UniProt
    P43146
    Pathways
    Regulation of Cell Size
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