CTNNA1 Protein (AA 2-906) (His tag)

Produktnummer ABIN3091997

Produktdetails

Target: CTNNA1 (Catenin (Cadherin-Associated Protein), alpha 1, 102kDa (CTNNA1))

Protein-Typ: Recombinant

Proteineigenschaft: AA 2-906

Spezies: Human

Quelle: Insektenzellen

Aufreinigungstag / Konjugat: Dieses CTNNA1 Protein ist gelabelt mit His tag.

Applikation: Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)

Sequenz: TAVHAGNINF KWDPKSLEIR TLAVERLLEP LVTQVTTLVN TNSKGPSNKK RGRSKKAHVL AASVEQATEN FLEKGDKIAK ESQFLKEELV AAVEDVRKQG DLMKAAAGEF ADDPCSSVKR GNMVRAARAL LSAVTRLLIL ADMADVYKLL VQLKVVEDGI LKLRNAGNEQ DLGIQYKALK PEVDKLNIMA AKRQQELKDV GHRDQMAAAR GILQKNVPIL YTASQACLQH PDVAAYKANR DLIYKQLQQA VTGISNAAQA TASDDASQHQ GGGGGELAYA LNNFDKQIIV DPLSFSEERF RPSLEERLES IISGAALMAD SSCTRDDRRE RIVAECNAVR QALQDLLSEY MGNAGRKERS DALNSAIDKM TKKTRDLRRQ LRKAVMDHVS DSFLETNVPL LVLIEAAKNG NEKEVKEYAQ VFREHANKLI EVANLACSIS NNEEGVKLVR MSASQLEALC PQVINAALAL AAKPQSKLAQ ENMDLFKEQW EKQVRVLTDA VDDITSIDDF LAVSENHILE DVNKCVIALQ EKDVDGLDRT AGAIRGRAAR VIHVVTSEMD NYEPGVYTEK VLEATKLLSN TVMPRFTEQV EAAVEALSSD PAQPMDENEF IDASRLVYDG IRDIRKAVLM IRTPEELDDS DFETEDFDVR SRTSVQTEDD QLIAGQSARA IMAQLPQEQK AKIAEQVASF QEEKSKLDAE VSKWDDSGND IIVLAKQMCM IMMEMTDFTR GKGPLKNTSD VISAAKKIAE AGSRMDKLGR TIADHCPDSA CKQDLLAYLQ RIALYCHQLN ICSKVKAEVQ NLGGELVVSG VDSAMSLIQA AKNLMNAVVQ TVKASYVAST KYQKSQGMAS LNLPAVSWKM KAPEKKPLVK REKQDETQTK IKRASQKKHV NPVQALSEFK AMDSI
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Produktmerkmale:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human CTNNA1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Protein is endotoxin free.

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Kommentare:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: CTNNA1 (Catenin (Cadherin-Associated Protein), alpha 1, 102kDa (CTNNA1))

Andere Bezeichnung: CTNNA1 (CTNNA1 Produkte)

Synonyme: CAP102 Protein, 2010010M04Rik Protein, AA517462 Protein, AI988031 Protein, Catna1 Protein, CG17947 Protein, CT39986 Protein, Dalpha-Cat Protein, Dalpha-cat Protein, DalphaC Protein, Dalphacat Protein, Dmcatp Protein, Dmel\\CG17947 Protein, a-Cat Protein, a-cat Protein, alpha-CAT Protein, alpha-cat Protein, alpha-catenin Protein, alpha-ctn Protein, alphaCAT Protein, alphaCat Protein, alphacat Protein, Alpha-Cat Protein, dgri_GLEANR_3829 Protein, DgriGH19548 Protein, GH19548 Protein, ctnna Protein, cap102 Protein, catna1 Protein, ctnna1 Protein, catenin alpha 1 Protein, catenin (cadherin associated protein), alpha 1 Protein, alpha Catenin Protein, catenin alpha-1 Protein, catenin (cadherin-associated protein), alpha 1 Protein, catenin alpha 1 S homeolog Protein, CTNNA1 Protein, Ctnna1 Protein, alpha-Cat Protein, Dgri\alpha-Cat Protein, Tsp_10127 Protein, ctnna1 Protein, ctnna1.S Protein

Hintergrund: Associates with the cytoplasmic domain of a variety of cadherins. The association of catenins to cadherins produces a complex which is linked to the actin filament network, and which seems to be of primary importance for cadherins cell-adhesion properties. Can associate with both E- and N-cadherins. Originally believed to be a stable component of E-cadherin/catenin adhesion complexes and to mediate the linkage of cadherins to the actin cytoskeleton at adherens junctions. In contrast, cortical actin was found to be much more dynamic than E-cadherin/catenin complexes and CTNNA1 was shown not to bind to F-actin when assembled in the complex suggesting a different linkage between actin and adherens junctions components. The homodimeric form may regulate actin filament assembly and inhibit actin branching by competing with the Arp2/3 complex for binding to actin filaments. May play a crucial role in cell differentiation.

Molekulargewicht: 100.9 kDa Including tag.

UniProt: P35221

Pathways: Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Maintenance of Protein Location