Calsyntenin 1 Protein (CLSTN1) (AA 29-859) (His tag)

Produktnummer ABIN3091943

Produktdetails

Target: Calsyntenin 1 (CLSTN1)

Protein-Typ: Recombinant

Proteineigenschaft: AA 29-859

Spezies: Human

Quelle: Insektenzellen

Aufreinigungstag / Konjugat: Dieses Calsyntenin 1 Protein ist gelabelt mit His tag.

Applikation: ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)

Sequenz: ARVNKHKPWL EPTYHGIVTE NDNTVLLDPP LIALDKDAPL RFAESFEVTV TKEGEICGFK IHGQNVPFDA VVVDKSTGEG VIRSKEKLDC ELQKDYSFTI QAYDCGKGPD GTNVKKSHKA TVHIQVNDVN EYAPVFKEKS YKATVIEGKQ YDSILRVEAV DADCSPQFSQ ICSYEIITPD VPFTVDKDGY IKNTEKLNYG KEHQYKLTVT AYDCGKKRAT EDVLVKISIK PTCTPGWQGW NNRIEYEPGT GALAVFPNIH LETCDEPVAS VQATVELETS HIGKGCDRDT YSEKSLHRLC GAAAGTAELL PSPSGSLNWT MGLPTDNGHD SDQVFEFNGT QAVRIPDGVV SVSPKEPFTI SVWMRHGPFG RKKETILCSS DKTDMNRHHY SLYVHGCRLI FLFRQDPSEE KKYRPAEFHW KLNQVCDEEW HHYVLNVEFP SVTLYVDGTS HEPFSVTEDY PLHPSKIETQ LVVGACWQEF SGVENDNETE PVTVASAGGD LHMTQFFRGN LAGLTLRSGK LADKKVIDCL YTCKEGLDLQ VLEDSGRGVQ IQAHPSQLVL TLEGEDLGEL DKAMQHISYL NSRQFPTPGI RRLKITSTIK CFNEATCISV PPVDGYVMVL QPEEPKISLS GVHHFARAAS EFESSEGVFL FPELRIISTI TREVEPEGDG AEDPTVQESL VSEEIVHDLD TCEVTVEGEE LNHEQESLEV DMARLQQKGI EVSSSELGMT FTGVDTMASY EEVLHLLRYR NWHARSLLDR KFKLICSELN GRYISNEFKV EVNVIHTANP MEHANHMAAQ PQFVHPEHRS FVDLSGHNLA NPHPFAVVPS T
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Produktmerkmale:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human CLSTN1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Protein is endotoxin free.

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Kommentare:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: Calsyntenin 1 (CLSTN1)

Andere Bezeichnung: CLSTN1 (CLSTN1 Produkte)

Synonyme: CALSYNTENIN-1 Protein, fm67g03 Protein, wu:fk52g02 Protein, wu:fm67g03 Protein, zgc:153744 Protein, zgc:56528 Protein, clstn1 Protein, 1810034E21Rik Protein, Cst-1 Protein, Cstn1 Protein, ALC-ALPHA Protein, CDHR12 Protein, CSTN1 Protein, PIK3CD Protein, XB31alpha Protein, alcalpha1 Protein, alcalpha2 Protein, calsyntenin 1 Protein, calsyntenin 3 Protein, beta-catenin-interacting protein 1 Protein, calsyntenin-1 Protein, CLSTN1 Protein, clstn1 Protein, clstn3 Protein, LOC100356725 Protein, LOC100435584 Protein, Clstn1 Protein, LOC479600 Protein

Hintergrund: Induces KLC1 association with vesicles and functions as a cargo in axonal anterograde transport. Complex formation with APBA2 and APP, stabilizes APP metabolism and enhances APBA2-mediated suppression of beta-APP40 secretion, due to the retardation of intracellular APP maturation. In complex with APBA2 and C99, a C-terminal APP fragment, abolishes C99 interaction with PSEN1 and thus APP C99 cleavage by gamma-secretase, most probably through stabilization of the direct interaction between APBA2 and APP. The intracellular fragment AlcICD suppresses APBB1-dependent transactivation stimulated by APP C-terminal intracellular fragment (AICD), most probably by competing with AICD for APBB1-binding. May modulate calcium-mediated postsynaptic signals (By similarity). {ECO:0000250, ECO:0000269|PubMed:12972431}.

Molekulargewicht: 93.9 kDa Including tag.

UniProt: O94985