NCAPG2 Protein (AA 1-1143) (Strep Tag)

Produktnummer ABIN3091858

Produktdetails

Target: NCAPG2 (Non-SMC Condensin II Complex, Subunit G2 (NCAPG2))

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-1143

Spezies: Human

Quelle: Tobacco (Nicotiana tabacum)

Aufreinigungstag / Konjugat: Dieses NCAPG2 Protein ist gelabelt mit Strep Tag.

Applikation: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Sequenz: MEKRETFVQA VSKELVGEFL QFVQLDKEAS DPFSLNELLD ELSRKQKEEL WQRLKNLLTD VLLESPVDGW QVVEAQGEDN METEHGSKMR KSIEIIYAIT SVILASVSVI NESENYEALL ECVIILNGIL YALPESERKL QSSIQDLCVT WWEKGLPAKE DTGKTAFVML LRRSLETKTG ADVCRLWRIH QALYCFDYDL EESGEIKDML LECFININYI KKEEGRRFLS CLFNWNINFI KMIHGTIKNQ LQGLQKSLMV YIAEIYFRAW KKASGKILEA IENDCIQDFM FHGIHLPRRS PVHSKVREVL SYFHHQKKVR QGVEEMLYRL YKPILWRGLK ARNSEVRSNA ALLFVEAFPI RDPNLHAIEM DSEIQKQFEE LYSLLEDPYP MVRSTGILGV CKITSKYWEM MPPTILIDLL KKVTGELAFD TSSADVRCSV FKCLPMILDN KLSHPLLEQL LPALRYSLHD NSEKVRVAFV DMLLKIKAVR AAKFWKICPM EHILVRLETD SRPVSRRLVS LIFNSFLPVN QPEEVWCERC VTLVQMNHAA ARRFYQYAHE HTACTNIAKL IHVIRHCLNA CIQRAVREPP EDEEEEDGRE KENVTVLDKT LSVNDVACMA GLLEIIVILW KSIDRSMENN KEAKLYTINK FASVLPEYLK VFKDDRCKIP LFMLMSFMPA SAVPPFSCGV ISTLRSREEG AVDKSYCTLL DCLCSWGQVG HILELVDNWL PTEHAQAKSN TASKGRVQIH DTRPVKPELA LVYIEYLLTH PKNRECLLSA PRKKLNHLLK ALETSKADLE SLLQTPGGKP RGFSEAAAPR AFGLHCRLSI HLQHKFCSEG KVYLSMLEDT GFWLESKILS FIQDQEEDYL KLHRVIYQQI IQTYLTVCKD VVMVGLGDHQ FQMQLLQRSL GIMQTVKGFF YVSLLLDILK EITGSSLIQK TDSDEEVAML LDTVQKVFQK MLECIARSFR KQPEEGLRLL YSVQRPLHEF ITAVQSRHTD TPVHRGVLST LIAGPVVEIS HQLRKVSDVE ELTPPEHLSD LPPFSRCLIG IIIKSSNVVR SFLDELKACV ASNDIEGIVC LTAAVHIILV INAGKHKSSK VREVAATVHR KLKTFMEITL EEDSIERFLY ESSSRTLGEL LNS
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Produktmerkmale: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin-Niveau: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Kommentare:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: NCAPG2 (Non-SMC Condensin II Complex, Subunit G2 (NCAPG2))

Andere Bezeichnung: NCAPG2 (NCAPG2 Produkte)

Synonyme: NCAPG2 Protein, LUZP5 Protein, id:ibd2775 Protein, zgc:162881 Protein, FAM62B Protein, CAP-G2 Protein, CAPG2 Protein, MTB Protein, hCAP-G2 Protein, 5830426I05Rik Protein, Luzp5 Protein, Mtb Protein, mCAP-G2 Protein, RGD1308406 Protein, non-SMC condensin II complex, subunit G2 Protein, non-SMC condensin II complex subunit G2 Protein, extended synaptotagmin 2 Protein, condensin-2 complex subunit G2 Protein, non-SMC condensin II complex subunit G2 L homeolog Protein, NCAPG2 Protein, ncapg2 Protein, ESYT2 Protein, LOC100401712 Protein, Ncapg2 Protein, ncapg2.L Protein

Hintergrund: Condensin-2 complex subunit G2 (Chromosome-associated protein G2) (CAP-G2) (hCAP-G2) (Leucine zipper protein 5) (Non-SMC condensin II complex subunit G2),FUNCTION: Regulatory subunit of the condensin-2 complex, a complex which establishes mitotic chromosome architecture and is involved in physical rigidity of the chromatid axis. {ECO:0000269|PubMed:14532007, ECO:0000269|PubMed:30609410}.

Molekulargewicht: 131.0 kDa

UniProt: Q86XI2