CLCN2 Protein (AA 2-898) (rho-1D4 tag)

Produktnummer ABIN3091843

Produktdetails

Target: CLCN2 (Chloride Channel 2 (CLCN2))

Protein-Typ: Recombinant

Proteineigenschaft: AA 2-898

Spezies: Human

Quelle: Insektenzellen

Aufreinigungstag / Konjugat: Dieses CLCN2 Protein ist gelabelt mit rho-1D4 tag.

Applikation: ELISA, Western Blotting (WB), SDS-PAGE (SDS), Crystallization (Crys)

Sequenz: AAAAAEEGME PRALQYEQTL MYGRYTQDLG AFAKEEAARI RLGGPEPWKG PPSSRAAPEL LEYGRSRCAR CRVCSVRCHK FLVSRVGEDW IFLVLLGLLM ALVSWVMDYA IAACLQAQQW MSRGLNTSIL LQYLAWVTYP VVLITFSAGF TQILAPQAVG SGIPEMKTIL RGVVLKEYLT LKTFIAKVIG LTCALGSGMP LGKEGPFVHI ASMCAALLSK FLSLFGGIYE NESRNTEMLA AACAVGVGCC FAAPIGGVLF SIEVTSTFFA VRNYWRGFFA ATFSAFIFRV LAVWNRDEET ITALFKTRFR LDFPFDLQEL PAFAVIGIAS GFGGALFVYL NRKIVQVMRK QKTINRFLMR KRLLFPALVT LLISTLTFPP GFGQFMAGQL SQKETLVTLF DNRTWVRQGL VEELEPPSTS QAWNPPRANV FLTLVIFILM KFWMSALATT IPVPCGAFMP VFVIGAAFGR LVGESMAAWF PDGIHTDSST YRIVPGGYAV VGAAALAGAV THTVSTAVIV FELTGQIAHI LPVMIAVILA NAVAQSLQPS LYDSIIRIKK LPYLPELGWG RHQQYRVRVE DIMVRDVPHV ALSCTFRDLR LALHRTKGRM LALVESPESM ILLGSIERSQ VVALLGAQLS PARRRQHMQE RRATQTSPLS DQEGPPTPEA SVCFQVNTED SAFPAARGET HKPLKPALKR GPSVTRNLGE SPTGSAESAG IALRSLFCGS PPPEAASEKL ESCEKRKLKR VRISLASDAD LEGEMSPEEI LEWEEQQLDE PVNFSDCKID PAPFQLVERT SLHKTHTIFS LLGVDHAYVT SIGRLIGIVT LKELRKAIEG SVTAQGVKVR PPLASFRDSA TSSSDTETTE VHALWGPHSR HGLPREGSPS DSDDKCQ
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Produktmerkmale:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human CLCN2 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Three step purification of membrane proteins expressed in baculovirus infected SF9 insect cells:

1. Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot.
2. The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot.
3. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Protein is endotoxin-free.

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Kommentare:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: CLCN2 (Chloride Channel 2 (CLCN2))

Andere Bezeichnung: CLCN2 (CLCN2 Produkte)

Synonyme: CLCN2 Protein, CLC2 Protein, LOC100150892 Protein, AL118368 Protein, ClC-2 Protein, Clc2 Protein, nmf240 Protein, CLC-2 Protein, CIC-2 Protein, ECA2 Protein, ECA3 Protein, EGI11 Protein, EGI3 Protein, EGMA Protein, EJM6 Protein, EJM8 Protein, clC-2 Protein, chloride voltage-gated channel 2 Protein, chloride channel, voltage-sensitive 2a Protein, chloride channel protein 2 Protein, chloride channel, voltage-sensitive 2 Protein, CLCN2 Protein, clcn2a Protein, EHI_142170 Protein, EHI_186860 Protein, Clcn2 Protein

Hintergrund: Voltage-gated chloride channel. Chloride channels have several functions including the regulation of cell volume, membrane potential stabilization, signal transduction and transepithelial transport. {ECO:0000269|PubMed:19153159, ECO:0000269|PubMed:19191339}.

Molekulargewicht: 99.6 kDa Including tag.

UniProt: P51788